The Symbiotic Bacterial Profile of Laboratory-Reared and Field-Caught <i>Aedes albopictus</i> Mosquitoes from Greece
The Asian tiger mosquito <i>Aedes albopictus</i> is a highly invasive species capable of transmitting human pathogens. For population management, the sterile insect technique (SIT) is considered an effective and sustainable alternative to conventional methods, such as insecticides and re...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/7/1486 |
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| Summary: | The Asian tiger mosquito <i>Aedes albopictus</i> is a highly invasive species capable of transmitting human pathogens. For population management, the sterile insect technique (SIT) is considered an effective and sustainable alternative to conventional methods, such as insecticides and reducing or eliminating breeding sites. The use of symbiotic bacteria to improve the application of SIT or design combined SIT/incompatible insect technique (IIT) approaches is currently considered. In this context, exploring the microbiota of local mosquito populations is crucial for identifying interesting components. This study employed 16S rRNA sequencing and microbiological methods to characterize the diversity of laboratory and wild <i>Ae. albopictus</i> in Greece. Differences were recorded between wild and lab-reared mosquitoes, with laboratory samples exhibiting higher diversity. Laboratory treatment, sex, and developmental stage also resulted in variations between communities. Populations reared in the same facility developed mostly similar bacterial profiles. Two geographically distant wild populations displayed similar bacterial profiles, characterized by seasonal changes in the relative abundance of <i>Pantoea</i> and <i>Zymobacter</i>. <i>Wolbachia</i> was dominant in most groups (63.7% relative abundance), especially in field-caught mosquitoes. It was identified with two strains, <i>w</i>AlbA (21.5%) and <i>w</i>AlbB (42.2%). Other frequent taxa included <i>Elizabethkingia</i>, <i>Asaia</i>, and <i>Serratia</i>. Blood feeding favored an increase in <i>Serratia</i> abundance. Various <i>Enterobacter</i>, <i>Klebsiella</i>, <i>Aeromonas</i>, and <i>Acinetobacter</i> strains were isolated from larval and adult mosquito extracts and could be further characterized as diet supplements. These findings suggest that the microbiota of local populations is highly variable due to multiple factors. However, they retain core elements shared across populations that may exhibit valuable nutritional or functional roles and could be exploited to improve SIT processes. |
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| ISSN: | 2076-2607 |