INFLUENCE OF APPROACHES TO ISOLATION OF ANIMAL BIOACTIVE SUBSTANCES ON ANTIMICROBIAL ACTION
This article highlights the problem of the use of chemical preservatives in the food industry. The prospects of implementation natural substances with an antimicrobial effect for prolonging shelf life and improving the quality of food products are also discussed. Methods for isolating of antimicrobi...
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| Format: | Article |
| Language: | English |
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The V.M. Gorbatov All-Russian Meat Research Institute
2018-09-01
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| Series: | Теория и практика переработки мяса |
| Subjects: | |
| Online Access: | https://www.meatjournal.ru/jour/article/view/95 |
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| author | Ekaterina A. Lukinova Elena A. Kotenkova Ekaterina K. Polischuk |
| author_facet | Ekaterina A. Lukinova Elena A. Kotenkova Ekaterina K. Polischuk |
| author_sort | Ekaterina A. Lukinova |
| collection | DOAJ |
| description | This article highlights the problem of the use of chemical preservatives in the food industry. The prospects of implementation natural substances with an antimicrobial effect for prolonging shelf life and improving the quality of food products are also discussed. Methods for isolating of antimicrobial substances from epithelial and mucous tissues of animal origin are proposed, taking into account the minimization of losses of their biological activity in accordance with the structure of protein-peptide molecules. Based on the results of the determination of antimicrobial activity against Listeria monocytogenes, Staphylococcus aureus and Pseudomonas aeruginosa using flow cytometry, stains Eva Green and PI, the most promising raw materials were determined and the feasibility of using weakly acid extraction followed by trypsinolysis for releasing antimicrobial substances from preprotein molecules and ultrafiltration for purification from high-molecular compounds were investigated. |
| format | Article |
| id | doaj-art-dbafe534ee024b86b54e264c9710f5ad |
| institution | Matheson Library |
| issn | 2414-438X 2414-441X |
| language | English |
| publishDate | 2018-09-01 |
| publisher | The V.M. Gorbatov All-Russian Meat Research Institute |
| record_format | Article |
| series | Теория и практика переработки мяса |
| spelling | doaj-art-dbafe534ee024b86b54e264c9710f5ad2025-08-04T13:11:44ZengThe V.M. Gorbatov All-Russian Meat Research InstituteТеория и практика переработки мяса2414-438X2414-441X2018-09-0133273510.21323/2414-438X-2018-3-3-27-3574INFLUENCE OF APPROACHES TO ISOLATION OF ANIMAL BIOACTIVE SUBSTANCES ON ANTIMICROBIAL ACTIONEkaterina A. Lukinova0Elena A. Kotenkova1Ekaterina K. Polischuk2V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, MoscowV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, MoscowV.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, MoscowThis article highlights the problem of the use of chemical preservatives in the food industry. The prospects of implementation natural substances with an antimicrobial effect for prolonging shelf life and improving the quality of food products are also discussed. Methods for isolating of antimicrobial substances from epithelial and mucous tissues of animal origin are proposed, taking into account the minimization of losses of their biological activity in accordance with the structure of protein-peptide molecules. Based on the results of the determination of antimicrobial activity against Listeria monocytogenes, Staphylococcus aureus and Pseudomonas aeruginosa using flow cytometry, stains Eva Green and PI, the most promising raw materials were determined and the feasibility of using weakly acid extraction followed by trypsinolysis for releasing antimicrobial substances from preprotein molecules and ultrafiltration for purification from high-molecular compounds were investigated.https://www.meatjournal.ru/jour/article/view/95antimicrobial substancesgram-positive bacteriagram-negative bacteriaflow cytometry |
| spellingShingle | Ekaterina A. Lukinova Elena A. Kotenkova Ekaterina K. Polischuk INFLUENCE OF APPROACHES TO ISOLATION OF ANIMAL BIOACTIVE SUBSTANCES ON ANTIMICROBIAL ACTION Теория и практика переработки мяса antimicrobial substances gram-positive bacteria gram-negative bacteria flow cytometry |
| title | INFLUENCE OF APPROACHES TO ISOLATION OF ANIMAL BIOACTIVE SUBSTANCES ON ANTIMICROBIAL ACTION |
| title_full | INFLUENCE OF APPROACHES TO ISOLATION OF ANIMAL BIOACTIVE SUBSTANCES ON ANTIMICROBIAL ACTION |
| title_fullStr | INFLUENCE OF APPROACHES TO ISOLATION OF ANIMAL BIOACTIVE SUBSTANCES ON ANTIMICROBIAL ACTION |
| title_full_unstemmed | INFLUENCE OF APPROACHES TO ISOLATION OF ANIMAL BIOACTIVE SUBSTANCES ON ANTIMICROBIAL ACTION |
| title_short | INFLUENCE OF APPROACHES TO ISOLATION OF ANIMAL BIOACTIVE SUBSTANCES ON ANTIMICROBIAL ACTION |
| title_sort | influence of approaches to isolation of animal bioactive substances on antimicrobial action |
| topic | antimicrobial substances gram-positive bacteria gram-negative bacteria flow cytometry |
| url | https://www.meatjournal.ru/jour/article/view/95 |
| work_keys_str_mv | AT ekaterinaalukinova influenceofapproachestoisolationofanimalbioactivesubstancesonantimicrobialaction AT elenaakotenkova influenceofapproachestoisolationofanimalbioactivesubstancesonantimicrobialaction AT ekaterinakpolischuk influenceofapproachestoisolationofanimalbioactivesubstancesonantimicrobialaction |