Analysis of changes in intestinal microbiota in female Wistar rats at the stage of breast cancer induction by N-methyl-N-nitrosourea
The intestinal microbiota, having enormous metabolic potential, makes a significant contribution to the physiological and pathological processes of humans and animals and is currently considered as an important factor in the pathogenesis of cancer. The aim of this study is to determine changes in th...
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Russian Academy of Sciences, Siberian Branch Publishing House
2024-08-01
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| Series: | Сибирский научный медицинский журнал |
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| Online Access: | https://sibmed.elpub.ru/jour/article/view/1621 |
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| author | V. N. Cherkas A. V. Kabakov A. F. Poveshchenko O. V. Kazakov A. A. Lelyak O. S. Kozlova |
| author_facet | V. N. Cherkas A. V. Kabakov A. F. Poveshchenko O. V. Kazakov A. A. Lelyak O. S. Kozlova |
| author_sort | V. N. Cherkas |
| collection | DOAJ |
| description | The intestinal microbiota, having enormous metabolic potential, makes a significant contribution to the physiological and pathological processes of humans and animals and is currently considered as an important factor in the pathogenesis of cancer. The aim of this study is to determine changes in the quantitative and qualitative composition of the intestinal microbiota in Wistar rats during chemical induction of breast cancer (BC). Material and methods. The work was performed on female Wistar rats (n = 40) aged 3 months, weighing 200–210 g, using cultural methods for studying fecal microbiota in intact rats (1 group) on the 1st, 14th, 35th days and in rats with induction of breast cancer and and in rats, whereby N-methyl-N-nitrosourea was administered to induce breast cancer (2 group) on the 1st (before injection of N-methyl-N-nitrosourea), 14th, 35th days after injection of N-methyl-N-nitrosourea. Results and discussion. In all experimental animals, representatives characteristic of the intestinal normobiota of warm-blooded animals predominated, namely: Bifidobacterium spp., Lactobacillus spp., Escherichia coli with pronounced enzymatic properties, Enterococcus spp., Clostridium spp. In addition, Staphylococcus spp., yeast-like fungi of the genus Candida and mold. Escherichia coli with reduced enzymatic activity was also detected. It was established that the isolated bacteria belonged to 3 types, 4 classes, 5 orders, 6 families, 6 genera of the bacterial domain. Also, 2 genera of fungi belonging to the order Saccharomycetales were isolated. The most significant changes in the composition of the intestinal microbiota were noted in rats with chemically induced breast cancer on the 35th day tumor induction: the appearance of pathogenic microflora in the intestine was revealed. |
| format | Article |
| id | doaj-art-db9a74a30ffb41b7a0fcc5cd44e339c5 |
| institution | Matheson Library |
| issn | 2410-2512 2410-2520 |
| language | Russian |
| publishDate | 2024-08-01 |
| publisher | Russian Academy of Sciences, Siberian Branch Publishing House |
| record_format | Article |
| series | Сибирский научный медицинский журнал |
| spelling | doaj-art-db9a74a30ffb41b7a0fcc5cd44e339c52025-07-15T11:26:38ZrusRussian Academy of Sciences, Siberian Branch Publishing HouseСибирский научный медицинский журнал2410-25122410-25202024-08-0144411912510.18699/SSMJ20240413663Analysis of changes in intestinal microbiota in female Wistar rats at the stage of breast cancer induction by N-methyl-N-nitrosoureaV. N. Cherkas0A. V. Kabakov1A. F. Poveshchenko2O. V. Kazakov3A. A. Lelyak4O. S. Kozlova5Research Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics of SB RASResearch Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics of SB RASResearch Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics of SB RASResearch Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics of SB RASNPF “Research Center”Veterinary Clinic “NovaVet”The intestinal microbiota, having enormous metabolic potential, makes a significant contribution to the physiological and pathological processes of humans and animals and is currently considered as an important factor in the pathogenesis of cancer. The aim of this study is to determine changes in the quantitative and qualitative composition of the intestinal microbiota in Wistar rats during chemical induction of breast cancer (BC). Material and methods. The work was performed on female Wistar rats (n = 40) aged 3 months, weighing 200–210 g, using cultural methods for studying fecal microbiota in intact rats (1 group) on the 1st, 14th, 35th days and in rats with induction of breast cancer and and in rats, whereby N-methyl-N-nitrosourea was administered to induce breast cancer (2 group) on the 1st (before injection of N-methyl-N-nitrosourea), 14th, 35th days after injection of N-methyl-N-nitrosourea. Results and discussion. In all experimental animals, representatives characteristic of the intestinal normobiota of warm-blooded animals predominated, namely: Bifidobacterium spp., Lactobacillus spp., Escherichia coli with pronounced enzymatic properties, Enterococcus spp., Clostridium spp. In addition, Staphylococcus spp., yeast-like fungi of the genus Candida and mold. Escherichia coli with reduced enzymatic activity was also detected. It was established that the isolated bacteria belonged to 3 types, 4 classes, 5 orders, 6 families, 6 genera of the bacterial domain. Also, 2 genera of fungi belonging to the order Saccharomycetales were isolated. The most significant changes in the composition of the intestinal microbiota were noted in rats with chemically induced breast cancer on the 35th day tumor induction: the appearance of pathogenic microflora in the intestine was revealed.https://sibmed.elpub.ru/jour/article/view/1621female wistar ratsintestinal microbiotainductionn-methyl-n-nitrosoureadysbiosisbreast cancercarcinogenesis |
| spellingShingle | V. N. Cherkas A. V. Kabakov A. F. Poveshchenko O. V. Kazakov A. A. Lelyak O. S. Kozlova Analysis of changes in intestinal microbiota in female Wistar rats at the stage of breast cancer induction by N-methyl-N-nitrosourea Сибирский научный медицинский журнал female wistar rats intestinal microbiota induction n-methyl-n-nitrosourea dysbiosis breast cancer carcinogenesis |
| title | Analysis of changes in intestinal microbiota in female Wistar rats at the stage of breast cancer induction by N-methyl-N-nitrosourea |
| title_full | Analysis of changes in intestinal microbiota in female Wistar rats at the stage of breast cancer induction by N-methyl-N-nitrosourea |
| title_fullStr | Analysis of changes in intestinal microbiota in female Wistar rats at the stage of breast cancer induction by N-methyl-N-nitrosourea |
| title_full_unstemmed | Analysis of changes in intestinal microbiota in female Wistar rats at the stage of breast cancer induction by N-methyl-N-nitrosourea |
| title_short | Analysis of changes in intestinal microbiota in female Wistar rats at the stage of breast cancer induction by N-methyl-N-nitrosourea |
| title_sort | analysis of changes in intestinal microbiota in female wistar rats at the stage of breast cancer induction by n methyl n nitrosourea |
| topic | female wistar rats intestinal microbiota induction n-methyl-n-nitrosourea dysbiosis breast cancer carcinogenesis |
| url | https://sibmed.elpub.ru/jour/article/view/1621 |
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