Histogram-based analysis for confocal microscope images with immunofluorescent staining: A graphical-user-interface tool
Immunofluorescent staining is widely utilized in biomedical research. However, reliable and reproducible quantification remains challenging and often depend on the experience of the raters heavily. As a result, variations caused by different raters or the same rater at different times confound quant...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016125001529 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839650352355344384 |
|---|---|
| author | Xiuli Yang Yuguo Li Zhiliang Wei |
| author_facet | Xiuli Yang Yuguo Li Zhiliang Wei |
| author_sort | Xiuli Yang |
| collection | DOAJ |
| description | Immunofluorescent staining is widely utilized in biomedical research. However, reliable and reproducible quantification remains challenging and often depend on the experience of the raters heavily. As a result, variations caused by different raters or the same rater at different times confound quantitative interpretations. In this study, we propose a histogram-based analytical method to explore the sources of signals at various intensities and, subsequently, identify the signals of interest using the histograms as a reference. Our method aims to alleviate inter-rater and intra-rater inconsistencies and improve the quantification of microscope images with immunofluorescent staining. • Identification of signal sources for image pixels at different intensities • Reduction of quantification variations using the proposed histogram-based analysis |
| format | Article |
| id | doaj-art-db8549f4360f4b67a37d23e52f0de856 |
| institution | Matheson Library |
| issn | 2215-0161 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj-art-db8549f4360f4b67a37d23e52f0de8562025-06-27T05:51:23ZengElsevierMethodsX2215-01612025-06-0114103306Histogram-based analysis for confocal microscope images with immunofluorescent staining: A graphical-user-interface toolXiuli Yang0Yuguo Li1Zhiliang Wei2Corresponding authors.; Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, United StatesDepartment of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, United StatesCorresponding authors.; Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, United StatesImmunofluorescent staining is widely utilized in biomedical research. However, reliable and reproducible quantification remains challenging and often depend on the experience of the raters heavily. As a result, variations caused by different raters or the same rater at different times confound quantitative interpretations. In this study, we propose a histogram-based analytical method to explore the sources of signals at various intensities and, subsequently, identify the signals of interest using the histograms as a reference. Our method aims to alleviate inter-rater and intra-rater inconsistencies and improve the quantification of microscope images with immunofluorescent staining. • Identification of signal sources for image pixels at different intensities • Reduction of quantification variations using the proposed histogram-based analysishttp://www.sciencedirect.com/science/article/pii/S2215016125001529Histogram-fitting-based quantification of immunofluorescent images |
| spellingShingle | Xiuli Yang Yuguo Li Zhiliang Wei Histogram-based analysis for confocal microscope images with immunofluorescent staining: A graphical-user-interface tool MethodsX Histogram-fitting-based quantification of immunofluorescent images |
| title | Histogram-based analysis for confocal microscope images with immunofluorescent staining: A graphical-user-interface tool |
| title_full | Histogram-based analysis for confocal microscope images with immunofluorescent staining: A graphical-user-interface tool |
| title_fullStr | Histogram-based analysis for confocal microscope images with immunofluorescent staining: A graphical-user-interface tool |
| title_full_unstemmed | Histogram-based analysis for confocal microscope images with immunofluorescent staining: A graphical-user-interface tool |
| title_short | Histogram-based analysis for confocal microscope images with immunofluorescent staining: A graphical-user-interface tool |
| title_sort | histogram based analysis for confocal microscope images with immunofluorescent staining a graphical user interface tool |
| topic | Histogram-fitting-based quantification of immunofluorescent images |
| url | http://www.sciencedirect.com/science/article/pii/S2215016125001529 |
| work_keys_str_mv | AT xiuliyang histogrambasedanalysisforconfocalmicroscopeimageswithimmunofluorescentstainingagraphicaluserinterfacetool AT yuguoli histogrambasedanalysisforconfocalmicroscopeimageswithimmunofluorescentstainingagraphicaluserinterfacetool AT zhiliangwei histogrambasedanalysisforconfocalmicroscopeimageswithimmunofluorescentstainingagraphicaluserinterfacetool |