Biosynthesis of anti-leishmanial natural products in callus cultures of Artemisia scoparia
Clinically, available synthetic chemotherapeutics in the treatment for leishmaniasis are associated with serious complications, such as toxicity and emergence of resistance. Natural products from plants can provide better remedies against the Leishmania parasite and can possibly minimize the associa...
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1593856 |
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| author | Reema Yousaf Mubarak Ali Khan Nazif Ullah Imdad Khan Obaid Hayat Muhammad Aamir Shehzad Irfan Khan Faqeer Taj Nizam Ud Din Asghar Khan Ijaz Naeem Huma Ali |
| author_facet | Reema Yousaf Mubarak Ali Khan Nazif Ullah Imdad Khan Obaid Hayat Muhammad Aamir Shehzad Irfan Khan Faqeer Taj Nizam Ud Din Asghar Khan Ijaz Naeem Huma Ali |
| author_sort | Reema Yousaf |
| collection | DOAJ |
| description | Clinically, available synthetic chemotherapeutics in the treatment for leishmaniasis are associated with serious complications, such as toxicity and emergence of resistance. Natural products from plants can provide better remedies against the Leishmania parasite and can possibly minimize the associated side effects. In this study, various extracts of the callus cultures of Artimisia scoparia established in response to different plant growth regulators (PGRs) were evaluated for their anti-leishmanial effects against Leishmania tropica promastigotes, followed by an investigation of the possible mechanism of action through reactive apoptosis assay using fluorescent microscopy. Amongst the different callus extracts, higher anti-leishmanial activity (IC50:19.13 µg/mL) was observed in the callus raised in-vitro in the presence of 6-Benzylaminopurine (BA) plus 2,4-Dichlorophenoxyacetic Acid (2,4-D) at the concentration of 1.5 mg/L, each. Further, the results of apoptosis assay showed a large number of early-stage apoptotic (EA) and late-stage apoptotic (LA) cells in the Leishmania under the effect of callus extract grown in-vitro at BA plus 2,4-D. For the determination of the potent natural products in the callus extracts responsible for the anti-leishmanial activity, extracts were subjected to Gas chromatography-mass spectrometry (GC-MS) for the metabolite analysis. Nonetheless, higher levels of the metabolites, such as nerolidol (22%), pelletierine (18%), aspidin (15%) and ascaridole (11%) were detected in the callus grown in vitro at BA plus 2,4-D (1.5 mg/L, each). This protocol determines a novel method of production of anti-leishmanial natural products through callus cultures of A. scoparia, a medicinal plant. |
| format | Article |
| id | doaj-art-db7fd454bece4e5c9c47fdd4a35553a8 |
| institution | Matheson Library |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
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| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-db7fd454bece4e5c9c47fdd4a35553a82025-07-21T21:22:18ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014711122113110.1080/21691401.2019.1593856Biosynthesis of anti-leishmanial natural products in callus cultures of Artemisia scopariaReema Yousaf0Mubarak Ali Khan1Nazif Ullah2Imdad Khan3Obaid Hayat4Muhammad Aamir Shehzad5Irfan Khan6Faqeer Taj7Nizam Ud Din8Asghar Khan9Ijaz Naeem10Huma Ali11Department of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Biotechnology, University of Swabi, Swabi, PakistanDepartment of Biotechnology, Bacha Khan University, Charsadda, PakistanClinically, available synthetic chemotherapeutics in the treatment for leishmaniasis are associated with serious complications, such as toxicity and emergence of resistance. Natural products from plants can provide better remedies against the Leishmania parasite and can possibly minimize the associated side effects. In this study, various extracts of the callus cultures of Artimisia scoparia established in response to different plant growth regulators (PGRs) were evaluated for their anti-leishmanial effects against Leishmania tropica promastigotes, followed by an investigation of the possible mechanism of action through reactive apoptosis assay using fluorescent microscopy. Amongst the different callus extracts, higher anti-leishmanial activity (IC50:19.13 µg/mL) was observed in the callus raised in-vitro in the presence of 6-Benzylaminopurine (BA) plus 2,4-Dichlorophenoxyacetic Acid (2,4-D) at the concentration of 1.5 mg/L, each. Further, the results of apoptosis assay showed a large number of early-stage apoptotic (EA) and late-stage apoptotic (LA) cells in the Leishmania under the effect of callus extract grown in-vitro at BA plus 2,4-D. For the determination of the potent natural products in the callus extracts responsible for the anti-leishmanial activity, extracts were subjected to Gas chromatography-mass spectrometry (GC-MS) for the metabolite analysis. Nonetheless, higher levels of the metabolites, such as nerolidol (22%), pelletierine (18%), aspidin (15%) and ascaridole (11%) were detected in the callus grown in vitro at BA plus 2,4-D (1.5 mg/L, each). This protocol determines a novel method of production of anti-leishmanial natural products through callus cultures of A. scoparia, a medicinal plant.https://www.tandfonline.com/doi/10.1080/21691401.2019.1593856Anti-leishmanialapoptosisplant growth regulatorsArtimisiacallus culturesecondary metabolites |
| spellingShingle | Reema Yousaf Mubarak Ali Khan Nazif Ullah Imdad Khan Obaid Hayat Muhammad Aamir Shehzad Irfan Khan Faqeer Taj Nizam Ud Din Asghar Khan Ijaz Naeem Huma Ali Biosynthesis of anti-leishmanial natural products in callus cultures of Artemisia scoparia Artificial Cells, Nanomedicine, and Biotechnology Anti-leishmanial apoptosis plant growth regulators Artimisia callus culture secondary metabolites |
| title | Biosynthesis of anti-leishmanial natural products in callus cultures of Artemisia scoparia |
| title_full | Biosynthesis of anti-leishmanial natural products in callus cultures of Artemisia scoparia |
| title_fullStr | Biosynthesis of anti-leishmanial natural products in callus cultures of Artemisia scoparia |
| title_full_unstemmed | Biosynthesis of anti-leishmanial natural products in callus cultures of Artemisia scoparia |
| title_short | Biosynthesis of anti-leishmanial natural products in callus cultures of Artemisia scoparia |
| title_sort | biosynthesis of anti leishmanial natural products in callus cultures of artemisia scoparia |
| topic | Anti-leishmanial apoptosis plant growth regulators Artimisia callus culture secondary metabolites |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1593856 |
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