Linkage map construction and QTL mapping for cold tolerance in Oryza rufipogon Griff. at early seedling stage
Cold stress is one of the major restraints for rice production. Cold tolerance is controlled by complex genetic factor. In this study, a backcross inbred lines (BILs) population derived from an inter-specific cross (Oryza sativa L.×O. rufipogon Griff.) was used for genetic linkage map construction a...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2016-12-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S209531191661465X |
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| author | Xiang-dong LUO Jun ZHAO Liang-fang DAI Fan-tao ZHANG Yi ZHOU Yong WAN Jian-kun XIE |
| author_facet | Xiang-dong LUO Jun ZHAO Liang-fang DAI Fan-tao ZHANG Yi ZHOU Yong WAN Jian-kun XIE |
| author_sort | Xiang-dong LUO |
| collection | DOAJ |
| description | Cold stress is one of the major restraints for rice production. Cold tolerance is controlled by complex genetic factor. In this study, a backcross inbred lines (BILs) population derived from an inter-specific cross (Oryza sativa L.×O. rufipogon Griff.) was used for genetic linkage map construction and quantitative trait locus (QTL) mapping. A linkage map consisting of 153 markers was constructed, spanning 1 596.8 cM with an average distance of 11.32 cM between the adjacent markers. Phenotypic evaluation of the parents and BILs under (6±1)°C cold stress revealed that the ability of cold tolerance in BILs at early seedling obeyed a skewed normal and continuous distribution. Fifteen QTLs on chromosomes 6, 7, 8, 11, and 12 were identified using survival percent (SP) and non death percent (NDP) as indicators of cold tolerance, which could explain 5.99 to 40.07% of the phenotypic variance, of which the LOD values ranged from 3.04 to 11.32. Four QTLs on chromosomes 3, 5 and 7 were detected using leaf conductivity (LC) and root conductivity (RC) as indicators of cold tolerance, ranging from 19.54 to 33.53% for the phenotypic variance explained and 2.54 to 6.12 for the LOD values. These results suggested that there might be multi major QTLs in O. rufipogon and some useful genes for cold tolerance have been transferred into cultivated rice, which would be helpful for cloning and utilizing the cold tolerance-responsive genes from wild rice. |
| format | Article |
| id | doaj-art-db7a5bfce5b641849932cddd4c9f9f3a |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-db7a5bfce5b641849932cddd4c9f9f3a2025-08-02T21:01:16ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192016-12-01151227032711Linkage map construction and QTL mapping for cold tolerance in Oryza rufipogon Griff. at early seedling stageXiang-dong LUO0Jun ZHAO1Liang-fang DAI2Fan-tao ZHANG3Yi ZHOU4Yong WAN5Jian-kun XIE6College of Life Science, Jiangxi Normal University, Nanchang 330022, P.R.China; Correspondence LUO Xiang-dong, Tel: +86-791-88120390College of Life Science, Jiangxi Normal University, Nanchang 330022, P.R.ChinaCollege of Life Science, Jiangxi Normal University, Nanchang 330022, P.R.ChinaCollege of Life Science, Jiangxi Normal University, Nanchang 330022, P.R.ChinaCollege of Life Science, Jiangxi Normal University, Nanchang 330022, P.R.ChinaRice Research Institute, Jiangxi Academy of Agricultural Science, Nanchang 330200, P.R.ChinaCollege of Life Science, Jiangxi Normal University, Nanchang 330022, P.R.China; XIE Jian-kun, Tel: +86-791-88120396Cold stress is one of the major restraints for rice production. Cold tolerance is controlled by complex genetic factor. In this study, a backcross inbred lines (BILs) population derived from an inter-specific cross (Oryza sativa L.×O. rufipogon Griff.) was used for genetic linkage map construction and quantitative trait locus (QTL) mapping. A linkage map consisting of 153 markers was constructed, spanning 1 596.8 cM with an average distance of 11.32 cM between the adjacent markers. Phenotypic evaluation of the parents and BILs under (6±1)°C cold stress revealed that the ability of cold tolerance in BILs at early seedling obeyed a skewed normal and continuous distribution. Fifteen QTLs on chromosomes 6, 7, 8, 11, and 12 were identified using survival percent (SP) and non death percent (NDP) as indicators of cold tolerance, which could explain 5.99 to 40.07% of the phenotypic variance, of which the LOD values ranged from 3.04 to 11.32. Four QTLs on chromosomes 3, 5 and 7 were detected using leaf conductivity (LC) and root conductivity (RC) as indicators of cold tolerance, ranging from 19.54 to 33.53% for the phenotypic variance explained and 2.54 to 6.12 for the LOD values. These results suggested that there might be multi major QTLs in O. rufipogon and some useful genes for cold tolerance have been transferred into cultivated rice, which would be helpful for cloning and utilizing the cold tolerance-responsive genes from wild rice.http://www.sciencedirect.com/science/article/pii/S209531191661465Xcommon wild ricecold tolerancequantitative trait loci (QTL)introgression |
| spellingShingle | Xiang-dong LUO Jun ZHAO Liang-fang DAI Fan-tao ZHANG Yi ZHOU Yong WAN Jian-kun XIE Linkage map construction and QTL mapping for cold tolerance in Oryza rufipogon Griff. at early seedling stage Journal of Integrative Agriculture common wild rice cold tolerance quantitative trait loci (QTL) introgression |
| title | Linkage map construction and QTL mapping for cold tolerance in Oryza rufipogon Griff. at early seedling stage |
| title_full | Linkage map construction and QTL mapping for cold tolerance in Oryza rufipogon Griff. at early seedling stage |
| title_fullStr | Linkage map construction and QTL mapping for cold tolerance in Oryza rufipogon Griff. at early seedling stage |
| title_full_unstemmed | Linkage map construction and QTL mapping for cold tolerance in Oryza rufipogon Griff. at early seedling stage |
| title_short | Linkage map construction and QTL mapping for cold tolerance in Oryza rufipogon Griff. at early seedling stage |
| title_sort | linkage map construction and qtl mapping for cold tolerance in oryza rufipogon griff at early seedling stage |
| topic | common wild rice cold tolerance quantitative trait loci (QTL) introgression |
| url | http://www.sciencedirect.com/science/article/pii/S209531191661465X |
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