Optimization of Specifications for Scaled-Up Fixed Rabies Virus Cultivation (“Moscow 3253” Strain) in Vero Cell Culture

Optimization of Specifications for Scaled-Up Fixed Rabies Virus Cultivation (“Moscow 3253” Strain) in Vero Cell Culture

/cell, using maintenance media 199 with admixture of 0.1 % human serum albumin or 2 % bovine serum. Optimum media volume in the roll-bottle for fixed rabies virus strain, “Moscow 3253”, cultivation is 200-400 ml. It depends upon the proliferating surface area. Specifications stated above provide for...

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Bibliographic Details
Main Authors: S. V. Generalov, E. G. Abramova, Zh. V. Matveeva, I. M. Zhulidov, L. V. Savitskaya, O. A. Lobovikova
Format: Article
Language:Russian
Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2014-06-01
Series:Проблемы особо опасных инфекций
Subjects:
бешенство
культура клеток
роллерное выращивание
антирабический иммуноглобулин
rabies
cell culture
roll-bottle cultivation
anti-rabies immunoglobulin
Online Access:https://journal.microbe.ru/jour/article/view/149
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Summary:/cell, using maintenance media 199 with admixture of 0.1 % human serum albumin or 2 % bovine serum. Optimum media volume in the roll-bottle for fixed rabies virus strain, “Moscow 3253”, cultivation is 200-400 ml. It depends upon the proliferating surface area. Specifications stated above provide for the obtainment of culture liquid with rabies virus titer - 1:256 - 1:512, if assayed in ELISA. This cultural virus is recommended as a basis for immunization material obtainment with a view to produce anti-rabies immunoglobulin from equine blood serum.
ISSN:0370-1069
2658-719X

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