IMMUNOGENETIC PECULIARITIES OF IL-1Β SYNTHESIS IN THE PATIENTS WITH PROTRACTED AND CHRONIC (RECURRENT) BACTERIAL INFLAMMATION OF UPPER RESPIRATORY WAYS (RHINOSINUSITIS)
We have studied distribution of IL-1β gene allelic variants (-511C>Т polymorphism in promoter region and IL-1β +3953C>Т polymorphism in exon 5) in sixty-two patients with recurrent and protracted rhinosinusitis, as well as their intercorrelations between allele frequencies, IL-1β level...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/237 |
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| author | L. F. Aznabaeva E. R. Sharipova N. A. Arefyeva A. G. Zainullina |
| author_facet | L. F. Aznabaeva E. R. Sharipova N. A. Arefyeva A. G. Zainullina |
| author_sort | L. F. Aznabaeva |
| collection | DOAJ |
| description | We have studied distribution of IL-1β gene allelic variants (-511C>Т polymorphism in promoter region and IL-1β +3953C>Т polymorphism in exon 5) in sixty-two patients with recurrent and protracted rhinosinusitis, as well as their intercorrelations between allele frequencies, IL-1β levels and predisposal for reccurence of chronic rhinosinusitis (CR). DNA samples were genotyped using PCR-RFLP analysis. IL-1β production by PHA-stimulated lymphocytes were determined in the cell supernates by means of quantitative enzyme immunoassay. In majority of patients (> 70%) with CR, decreased production of IL-1β was found, irrespectively of disease recurrence. Frequency of a «low-producer» homozygous *С/*С genotype +3953C>Т of IL-1β gene was two-fold higher in the patients with CR than in healthy controls (OR=3.4). In patients with protracted, recurrence-free patients, this parameter showed only a tendency to increase. Decreased production of IL-1β was associated with homozygous *С/*С genotype +3953C>Т of IL-1β gene (OR = 3,4), whereas high levels correlated with heterozygous С/*T genotype (OR = 7,6).Thus, low IL-1β production in carriers of homozygous *С/*С of +3953 polymorphic locus of IL-1β gene represents a diagnostic marker reflecting a risk of recurrent disease. Hence, a pathogenesis-based therapy of purulent rhinosinusitis with protracted and recurrent clinical course is possible by means of recombinant IL-1β (e.g., Betaleukin, St.Petersburg Institute for Highly Pure Biological Preparations), being a highly effective approach which favors decreased recurrence of disorder. |
| format | Article |
| id | doaj-art-db61d57876804bfcbd35bcc05b22908d |
| institution | Matheson Library |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-db61d57876804bfcbd35bcc05b22908d2025-08-04T14:30:29ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0194-553554010.15789/1563-0625-2007-4-5-535-540234IMMUNOGENETIC PECULIARITIES OF IL-1Β SYNTHESIS IN THE PATIENTS WITH PROTRACTED AND CHRONIC (RECURRENT) BACTERIAL INFLAMMATION OF UPPER RESPIRATORY WAYS (RHINOSINUSITIS)L. F. Aznabaeva0E. R. Sharipova1N. A. Arefyeva2A. G. Zainullina3Башкирский государственный медицинский университет Республиканская клиническая больница им. Г.Г. Куватова, г. УфаБашкирский государственный медицинский университетБашкирский государственный медицинский университетИнститут биохимии и генетики УфНЦ РАНWe have studied distribution of IL-1β gene allelic variants (-511C>Т polymorphism in promoter region and IL-1β +3953C>Т polymorphism in exon 5) in sixty-two patients with recurrent and protracted rhinosinusitis, as well as their intercorrelations between allele frequencies, IL-1β levels and predisposal for reccurence of chronic rhinosinusitis (CR). DNA samples were genotyped using PCR-RFLP analysis. IL-1β production by PHA-stimulated lymphocytes were determined in the cell supernates by means of quantitative enzyme immunoassay. In majority of patients (> 70%) with CR, decreased production of IL-1β was found, irrespectively of disease recurrence. Frequency of a «low-producer» homozygous *С/*С genotype +3953C>Т of IL-1β gene was two-fold higher in the patients with CR than in healthy controls (OR=3.4). In patients with protracted, recurrence-free patients, this parameter showed only a tendency to increase. Decreased production of IL-1β was associated with homozygous *С/*С genotype +3953C>Т of IL-1β gene (OR = 3,4), whereas high levels correlated with heterozygous С/*T genotype (OR = 7,6).Thus, low IL-1β production in carriers of homozygous *С/*С of +3953 polymorphic locus of IL-1β gene represents a diagnostic marker reflecting a risk of recurrent disease. Hence, a pathogenesis-based therapy of purulent rhinosinusitis with protracted and recurrent clinical course is possible by means of recombinant IL-1β (e.g., Betaleukin, St.Petersburg Institute for Highly Pure Biological Preparations), being a highly effective approach which favors decreased recurrence of disorder.https://www.mimmun.ru/mimmun/article/view/237recurrent and protracted rhinosinusitisil-1βgenotype |
| spellingShingle | L. F. Aznabaeva E. R. Sharipova N. A. Arefyeva A. G. Zainullina IMMUNOGENETIC PECULIARITIES OF IL-1Β SYNTHESIS IN THE PATIENTS WITH PROTRACTED AND CHRONIC (RECURRENT) BACTERIAL INFLAMMATION OF UPPER RESPIRATORY WAYS (RHINOSINUSITIS) Медицинская иммунология recurrent and protracted rhinosinusitis il-1β genotype |
| title | IMMUNOGENETIC PECULIARITIES OF IL-1Β SYNTHESIS IN THE PATIENTS WITH PROTRACTED AND CHRONIC (RECURRENT) BACTERIAL INFLAMMATION OF UPPER RESPIRATORY WAYS (RHINOSINUSITIS) |
| title_full | IMMUNOGENETIC PECULIARITIES OF IL-1Β SYNTHESIS IN THE PATIENTS WITH PROTRACTED AND CHRONIC (RECURRENT) BACTERIAL INFLAMMATION OF UPPER RESPIRATORY WAYS (RHINOSINUSITIS) |
| title_fullStr | IMMUNOGENETIC PECULIARITIES OF IL-1Β SYNTHESIS IN THE PATIENTS WITH PROTRACTED AND CHRONIC (RECURRENT) BACTERIAL INFLAMMATION OF UPPER RESPIRATORY WAYS (RHINOSINUSITIS) |
| title_full_unstemmed | IMMUNOGENETIC PECULIARITIES OF IL-1Β SYNTHESIS IN THE PATIENTS WITH PROTRACTED AND CHRONIC (RECURRENT) BACTERIAL INFLAMMATION OF UPPER RESPIRATORY WAYS (RHINOSINUSITIS) |
| title_short | IMMUNOGENETIC PECULIARITIES OF IL-1Β SYNTHESIS IN THE PATIENTS WITH PROTRACTED AND CHRONIC (RECURRENT) BACTERIAL INFLAMMATION OF UPPER RESPIRATORY WAYS (RHINOSINUSITIS) |
| title_sort | immunogenetic peculiarities of il 1β synthesis in the patients with protracted and chronic recurrent bacterial inflammation of upper respiratory ways rhinosinusitis |
| topic | recurrent and protracted rhinosinusitis il-1β genotype |
| url | https://www.mimmun.ru/mimmun/article/view/237 |
| work_keys_str_mv | AT lfaznabaeva immunogeneticpeculiaritiesofil1bsynthesisinthepatientswithprotractedandchronicrecurrentbacterialinflammationofupperrespiratorywaysrhinosinusitis AT ersharipova immunogeneticpeculiaritiesofil1bsynthesisinthepatientswithprotractedandchronicrecurrentbacterialinflammationofupperrespiratorywaysrhinosinusitis AT naarefyeva immunogeneticpeculiaritiesofil1bsynthesisinthepatientswithprotractedandchronicrecurrentbacterialinflammationofupperrespiratorywaysrhinosinusitis AT agzainullina immunogeneticpeculiaritiesofil1bsynthesisinthepatientswithprotractedandchronicrecurrentbacterialinflammationofupperrespiratorywaysrhinosinusitis |