<i>Lactobacillus fermentum</i> ZC529 Protects Intestinal Epithelial Barrier Integrity by Activating the Keap1-Nrf2 Signaling Pathway and Inhibiting the NF-κB Signaling Pathway

<i>Lactobacillus fermentum</i> ZC529 Protects Intestinal Epithelial Barrier Integrity by Activating the Keap1-Nrf2 Signaling Pathway and Inhibiting the NF-κB Signaling Pathway

The probiotic bacteria <i>Lactobacillus fermentum</i> ZC529 (<i>L.f</i> ZC529) has been identified from the colon of the Diannan small-ear (DSE) pig, but its intestinal protective function still lacks investigation. Here, we established a dextran sodium sulfate (DSS)-induced...

Full description

Saved in:
Bibliographic Details
Main Authors: Zian Yuan, Lang Huang, Zhenguo Hu, Junhao Deng, Yehui Duan, Qian Jiang, Bi’e Tan, Xiaokang Ma, Chen Zhang, Xiongzhuo Tang
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Antioxidants
Subjects:
<i>Lactobacillus fermentum</i>
antioxidant
anti-inflammation
Nrf2
NF-κB
Online Access:https://www.mdpi.com/2076-3921/14/6/732
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The probiotic bacteria <i>Lactobacillus fermentum</i> ZC529 (<i>L.f</i> ZC529) has been identified from the colon of the Diannan small-ear (DSE) pig, but its intestinal protective function still lacks investigation. Here, we established a dextran sodium sulfate (DSS)-induced intestinal oxidative stress model in both <i>Drosophila</i> and porcine small intestinal epithelial (IPEC-J2) cell lines to explore the anti-oxidative and anti-inflammatory effects of <i>L.f</i> ZC529. The data showed that the intestinal colonization of <i>L.f</i> ZC529 counteracted DSS-induced intestinal oxidative stress and excessive reactive oxygen species (ROS) generation by activation of the CncC pathway, a homology of the nuclear factor erythroid 2-related factor 2 (Nrf2) in mammalian systems. Moreover, <i>L.f</i> ZC529 supplementation prevented flies from DSS-induced intestinal barrier damage, inflammation, abnormal excretory function, and shortened lifespan. Finally, <i>L.f</i> ZC529 also attenuated DSS-induced intestinal injury in the IPEC-J2 cell line by activating the Keap1-Nrf2 signaling and inhibiting the NF-κB signaling pathways. Together, this study unraveled the profound intestinal protective function of <i>L.f</i> ZC529 and provides its potential application as a new antioxidant in improving animal intestinal health as well as in developing a new probiotic in the food industry.
ISSN:2076-3921

Similar Items