Gene expression response under thermal stress in two Hawaiian corals is dominated by ploidy and genotype
Abstract Transcriptome data are frequently used to investigate coral bleaching; however, the factors controlling gene expression in natural populations of these species are poorly understood. We studied two corals, Montipora capitata and Pocillopora acuta, that inhabit the sheltered Kāne'ohe Ba...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-07-01
|
| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.70037 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839618475547426816 |
|---|---|
| author | Erin E. Chille Timothy G. Stephens Deeksha Misri Emma L. Strand Hollie M. Putnam Debashish Bhattacharya |
| author_facet | Erin E. Chille Timothy G. Stephens Deeksha Misri Emma L. Strand Hollie M. Putnam Debashish Bhattacharya |
| author_sort | Erin E. Chille |
| collection | DOAJ |
| description | Abstract Transcriptome data are frequently used to investigate coral bleaching; however, the factors controlling gene expression in natural populations of these species are poorly understood. We studied two corals, Montipora capitata and Pocillopora acuta, that inhabit the sheltered Kāne'ohe Bay, Hawai'i. M. capitata colonies in the bay are outbreeding diploids, whereas P. acuta is a mixture of clonal diploids and triploids. Populations were sampled from six reefs and subjected to either control (no stress), thermal stress, pH stress, or combined pH and thermal stress treatments. RNA‐seq data were generated to test two competing hypotheses: (1) gene expression is largely independent of genotype, reflecting a shared treatment‐driven response (TDE) or, (2) genotype dominates gene expression, regardless of treatment (GDE). Our results strongly support the GDE model, even under severe stress. We suggest that post‐transcriptional processes (e.g., control of translation, protein turnover) modify the signal from the transcriptome, and may underlie the observed differences in coral bleaching sensitivity via the downstream proteome and metabolome. |
| format | Article |
| id | doaj-art-d8b649c2d53e4bf392dcec7c611b48c9 |
| institution | Matheson Library |
| issn | 2045-7758 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj-art-d8b649c2d53e4bf392dcec7c611b48c92025-07-24T00:21:19ZengWileyEcology and Evolution2045-77582024-07-01147n/an/a10.1002/ece3.70037Gene expression response under thermal stress in two Hawaiian corals is dominated by ploidy and genotypeErin E. Chille0Timothy G. Stephens1Deeksha Misri2Emma L. Strand3Hollie M. Putnam4Debashish Bhattacharya5Department of Biochemistry and Microbiology Rutgers University New Brunswick New Jersey USADepartment of Biochemistry and Microbiology Rutgers University New Brunswick New Jersey USADepartment of Genetics Rutgers University New Brunswick New Jersey USADepartment of Biological Sciences University of Rhode Island Kingston Rhode Island USADepartment of Biological Sciences University of Rhode Island Kingston Rhode Island USADepartment of Biochemistry and Microbiology Rutgers University New Brunswick New Jersey USAAbstract Transcriptome data are frequently used to investigate coral bleaching; however, the factors controlling gene expression in natural populations of these species are poorly understood. We studied two corals, Montipora capitata and Pocillopora acuta, that inhabit the sheltered Kāne'ohe Bay, Hawai'i. M. capitata colonies in the bay are outbreeding diploids, whereas P. acuta is a mixture of clonal diploids and triploids. Populations were sampled from six reefs and subjected to either control (no stress), thermal stress, pH stress, or combined pH and thermal stress treatments. RNA‐seq data were generated to test two competing hypotheses: (1) gene expression is largely independent of genotype, reflecting a shared treatment‐driven response (TDE) or, (2) genotype dominates gene expression, regardless of treatment (GDE). Our results strongly support the GDE model, even under severe stress. We suggest that post‐transcriptional processes (e.g., control of translation, protein turnover) modify the signal from the transcriptome, and may underlie the observed differences in coral bleaching sensitivity via the downstream proteome and metabolome.https://doi.org/10.1002/ece3.70037asexual reproductionclonal lineagescoralgene expressionprincipal component analysistriploidy |
| spellingShingle | Erin E. Chille Timothy G. Stephens Deeksha Misri Emma L. Strand Hollie M. Putnam Debashish Bhattacharya Gene expression response under thermal stress in two Hawaiian corals is dominated by ploidy and genotype Ecology and Evolution asexual reproduction clonal lineages coral gene expression principal component analysis triploidy |
| title | Gene expression response under thermal stress in two Hawaiian corals is dominated by ploidy and genotype |
| title_full | Gene expression response under thermal stress in two Hawaiian corals is dominated by ploidy and genotype |
| title_fullStr | Gene expression response under thermal stress in two Hawaiian corals is dominated by ploidy and genotype |
| title_full_unstemmed | Gene expression response under thermal stress in two Hawaiian corals is dominated by ploidy and genotype |
| title_short | Gene expression response under thermal stress in two Hawaiian corals is dominated by ploidy and genotype |
| title_sort | gene expression response under thermal stress in two hawaiian corals is dominated by ploidy and genotype |
| topic | asexual reproduction clonal lineages coral gene expression principal component analysis triploidy |
| url | https://doi.org/10.1002/ece3.70037 |
| work_keys_str_mv | AT erinechille geneexpressionresponseunderthermalstressintwohawaiiancoralsisdominatedbyploidyandgenotype AT timothygstephens geneexpressionresponseunderthermalstressintwohawaiiancoralsisdominatedbyploidyandgenotype AT deekshamisri geneexpressionresponseunderthermalstressintwohawaiiancoralsisdominatedbyploidyandgenotype AT emmalstrand geneexpressionresponseunderthermalstressintwohawaiiancoralsisdominatedbyploidyandgenotype AT holliemputnam geneexpressionresponseunderthermalstressintwohawaiiancoralsisdominatedbyploidyandgenotype AT debashishbhattacharya geneexpressionresponseunderthermalstressintwohawaiiancoralsisdominatedbyploidyandgenotype |