IDENTIFICATION OF ISLET CAPACITY IN DONOR’S PANCREAS USING IMMUNOMORPHOLOGICAL ANALYSIS
The aim of the work was detailed morphological investigations of donor pancreas (DP) for the study of possibilities of maximal selection of islet tissue suitable for transplantation to a patient of diabetes mellitus type 1.Materials and methods. Eight DPs were received as a result of multiorgan dona...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2016-04-01
|
| Series: | Вестник трансплантологии и искусственных органов |
| Subjects: | |
| Online Access: | https://journal.transpl.ru/vtio/article/view/616 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839573943414947840 |
|---|---|
| author | N. N. Skaletskiy L. A. Kirsanova G. N. Bubentsova N. V. Baranova G. N. Skaletskaya V. I. Sevastianov |
| author_facet | N. N. Skaletskiy L. A. Kirsanova G. N. Bubentsova N. V. Baranova G. N. Skaletskaya V. I. Sevastianov |
| author_sort | N. N. Skaletskiy |
| collection | DOAJ |
| description | The aim of the work was detailed morphological investigations of donor pancreas (DP) for the study of possibilities of maximal selection of islet tissue suitable for transplantation to a patient of diabetes mellitus type 1.Materials and methods. Eight DPs were received as a result of multiorgan donation. Morphological investigations were performed by means of histological and special immunohistochemical methods.Results. The Majority of islets were revealed in the tail part of the DP. Besides typical Langerhans islets with predominance of mosaically located beta cells, the accumulations of islet cells forming so-called interlobular (perilobular) islets were revealed in the layers of interlobular connecting tissue. In addition, in the cells of ductal epithelium nestin which is a marker of progenitor cells was revealed.Conclusion. To obtain the maximal potential of islet tissue from DP it is necessary to use interlobular located islets as well as to use progenitor cells of pancreas, which have the ability to transdifferentiate into islet cells. |
| format | Article |
| id | doaj-art-d88ee9c67d714e8e967dc26355ac6b59 |
| institution | Matheson Library |
| issn | 1995-1191 |
| language | Russian |
| publishDate | 2016-04-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Вестник трансплантологии и искусственных органов |
| spelling | doaj-art-d88ee9c67d714e8e967dc26355ac6b592025-08-04T14:02:07ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovВестник трансплантологии и искусственных органов1995-11912016-04-01181323710.15825/1995-1191-2016-1-32-37523IDENTIFICATION OF ISLET CAPACITY IN DONOR’S PANCREAS USING IMMUNOMORPHOLOGICAL ANALYSISN. N. Skaletskiy0L. A. Kirsanova1G. N. Bubentsova2N. V. Baranova3G. N. Skaletskaya4V. I. Sevastianov5V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian FederationV.I. Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian FederationV.I. Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian FederationV.I. Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian FederationV.I. Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian FederationV.I. Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian FederationThe aim of the work was detailed morphological investigations of donor pancreas (DP) for the study of possibilities of maximal selection of islet tissue suitable for transplantation to a patient of diabetes mellitus type 1.Materials and methods. Eight DPs were received as a result of multiorgan donation. Morphological investigations were performed by means of histological and special immunohistochemical methods.Results. The Majority of islets were revealed in the tail part of the DP. Besides typical Langerhans islets with predominance of mosaically located beta cells, the accumulations of islet cells forming so-called interlobular (perilobular) islets were revealed in the layers of interlobular connecting tissue. In addition, in the cells of ductal epithelium nestin which is a marker of progenitor cells was revealed.Conclusion. To obtain the maximal potential of islet tissue from DP it is necessary to use interlobular located islets as well as to use progenitor cells of pancreas, which have the ability to transdifferentiate into islet cells.https://journal.transpl.ru/vtio/article/view/616donor pancreasimmunomorphological investigationsinterlobular isletsprogenitor cells |
| spellingShingle | N. N. Skaletskiy L. A. Kirsanova G. N. Bubentsova N. V. Baranova G. N. Skaletskaya V. I. Sevastianov IDENTIFICATION OF ISLET CAPACITY IN DONOR’S PANCREAS USING IMMUNOMORPHOLOGICAL ANALYSIS Вестник трансплантологии и искусственных органов donor pancreas immunomorphological investigations interlobular islets progenitor cells |
| title | IDENTIFICATION OF ISLET CAPACITY IN DONOR’S PANCREAS USING IMMUNOMORPHOLOGICAL ANALYSIS |
| title_full | IDENTIFICATION OF ISLET CAPACITY IN DONOR’S PANCREAS USING IMMUNOMORPHOLOGICAL ANALYSIS |
| title_fullStr | IDENTIFICATION OF ISLET CAPACITY IN DONOR’S PANCREAS USING IMMUNOMORPHOLOGICAL ANALYSIS |
| title_full_unstemmed | IDENTIFICATION OF ISLET CAPACITY IN DONOR’S PANCREAS USING IMMUNOMORPHOLOGICAL ANALYSIS |
| title_short | IDENTIFICATION OF ISLET CAPACITY IN DONOR’S PANCREAS USING IMMUNOMORPHOLOGICAL ANALYSIS |
| title_sort | identification of islet capacity in donor s pancreas using immunomorphological analysis |
| topic | donor pancreas immunomorphological investigations interlobular islets progenitor cells |
| url | https://journal.transpl.ru/vtio/article/view/616 |
| work_keys_str_mv | AT nnskaletskiy identificationofisletcapacityindonorspancreasusingimmunomorphologicalanalysis AT lakirsanova identificationofisletcapacityindonorspancreasusingimmunomorphologicalanalysis AT gnbubentsova identificationofisletcapacityindonorspancreasusingimmunomorphologicalanalysis AT nvbaranova identificationofisletcapacityindonorspancreasusingimmunomorphologicalanalysis AT gnskaletskaya identificationofisletcapacityindonorspancreasusingimmunomorphologicalanalysis AT visevastianov identificationofisletcapacityindonorspancreasusingimmunomorphologicalanalysis |