Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos.
Prior GWAS have identified loci associated with red blood cell (RBC) traits in populations of European, African, and Asian ancestry. These studies have not included individuals with an Amerindian ancestral background, such as Hispanics/Latinos, nor evaluated the full spectrum of genomic variation be...
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Public Library of Science (PLoS)
2017-04-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1006760 |
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| author | Chani J Hodonsky Deepti Jain Ursula M Schick Jean V Morrison Lisa Brown Caitlin P McHugh Claudia Schurmann Diane D Chen Yong Mei Liu Paul L Auer Cecilia A Laurie Kent D Taylor Brian L Browning Yun Li George Papanicolaou Jerome I Rotter Ryo Kurita Yukio Nakamura Sharon R Browning Ruth J F Loos Kari E North Cathy C Laurie Timothy A Thornton Nathan Pankratz Daniel E Bauer Tamar Sofer Alex P Reiner |
| author_facet | Chani J Hodonsky Deepti Jain Ursula M Schick Jean V Morrison Lisa Brown Caitlin P McHugh Claudia Schurmann Diane D Chen Yong Mei Liu Paul L Auer Cecilia A Laurie Kent D Taylor Brian L Browning Yun Li George Papanicolaou Jerome I Rotter Ryo Kurita Yukio Nakamura Sharon R Browning Ruth J F Loos Kari E North Cathy C Laurie Timothy A Thornton Nathan Pankratz Daniel E Bauer Tamar Sofer Alex P Reiner |
| author_sort | Chani J Hodonsky |
| collection | DOAJ |
| description | Prior GWAS have identified loci associated with red blood cell (RBC) traits in populations of European, African, and Asian ancestry. These studies have not included individuals with an Amerindian ancestral background, such as Hispanics/Latinos, nor evaluated the full spectrum of genomic variation beyond single nucleotide variants. Using a custom genotyping array enriched for Amerindian ancestral content and 1000 Genomes imputation, we performed GWAS in 12,502 participants of Hispanic Community Health Study and Study of Latinos (HCHS/SOL) for hematocrit, hemoglobin, RBC count, RBC distribution width (RDW), and RBC indices. Approximately 60% of previously reported RBC trait loci generalized to HCHS/SOL Hispanics/Latinos, including African ancestral alpha- and beta-globin gene variants. In addition to the known 3.8kb alpha-globin copy number variant, we identified an Amerindian ancestral association in an alpha-globin regulatory region on chromosome 16p13.3 for mean corpuscular volume and mean corpuscular hemoglobin. We also discovered and replicated three genome-wide significant variants in previously unreported loci for RDW (SLC12A2 rs17764730, PSMB5 rs941718), and hematocrit (PROX1 rs3754140). Among the proxy variants at the SLC12A2 locus we identified rs3812049, located in a bi-directional promoter between SLC12A2 (which encodes a red cell membrane ion-transport protein) and an upstream anti-sense long-noncoding RNA, LINC01184, as the likely causal variant. We further demonstrate that disruption of the regulatory element harboring rs3812049 affects transcription of SLC12A2 and LINC01184 in human erythroid progenitor cells. Together, these results reinforce the importance of genetic study of diverse ancestral populations, in particular Hispanics/Latinos. |
| format | Article |
| id | doaj-art-d79b27a0384a489caf7bdbe0fd8fe84d |
| institution | Matheson Library |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2017-04-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS Genetics |
| spelling | doaj-art-d79b27a0384a489caf7bdbe0fd8fe84d2025-06-26T05:30:57ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042017-04-01134e100676010.1371/journal.pgen.1006760Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos.Chani J HodonskyDeepti JainUrsula M SchickJean V MorrisonLisa BrownCaitlin P McHughClaudia SchurmannDiane D ChenYong Mei LiuPaul L AuerCecilia A LaurieKent D TaylorBrian L BrowningYun LiGeorge PapanicolaouJerome I RotterRyo KuritaYukio NakamuraSharon R BrowningRuth J F LoosKari E NorthCathy C LaurieTimothy A ThorntonNathan PankratzDaniel E BauerTamar SoferAlex P ReinerPrior GWAS have identified loci associated with red blood cell (RBC) traits in populations of European, African, and Asian ancestry. These studies have not included individuals with an Amerindian ancestral background, such as Hispanics/Latinos, nor evaluated the full spectrum of genomic variation beyond single nucleotide variants. Using a custom genotyping array enriched for Amerindian ancestral content and 1000 Genomes imputation, we performed GWAS in 12,502 participants of Hispanic Community Health Study and Study of Latinos (HCHS/SOL) for hematocrit, hemoglobin, RBC count, RBC distribution width (RDW), and RBC indices. Approximately 60% of previously reported RBC trait loci generalized to HCHS/SOL Hispanics/Latinos, including African ancestral alpha- and beta-globin gene variants. In addition to the known 3.8kb alpha-globin copy number variant, we identified an Amerindian ancestral association in an alpha-globin regulatory region on chromosome 16p13.3 for mean corpuscular volume and mean corpuscular hemoglobin. We also discovered and replicated three genome-wide significant variants in previously unreported loci for RDW (SLC12A2 rs17764730, PSMB5 rs941718), and hematocrit (PROX1 rs3754140). Among the proxy variants at the SLC12A2 locus we identified rs3812049, located in a bi-directional promoter between SLC12A2 (which encodes a red cell membrane ion-transport protein) and an upstream anti-sense long-noncoding RNA, LINC01184, as the likely causal variant. We further demonstrate that disruption of the regulatory element harboring rs3812049 affects transcription of SLC12A2 and LINC01184 in human erythroid progenitor cells. Together, these results reinforce the importance of genetic study of diverse ancestral populations, in particular Hispanics/Latinos.https://doi.org/10.1371/journal.pgen.1006760 |
| spellingShingle | Chani J Hodonsky Deepti Jain Ursula M Schick Jean V Morrison Lisa Brown Caitlin P McHugh Claudia Schurmann Diane D Chen Yong Mei Liu Paul L Auer Cecilia A Laurie Kent D Taylor Brian L Browning Yun Li George Papanicolaou Jerome I Rotter Ryo Kurita Yukio Nakamura Sharon R Browning Ruth J F Loos Kari E North Cathy C Laurie Timothy A Thornton Nathan Pankratz Daniel E Bauer Tamar Sofer Alex P Reiner Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos. PLoS Genetics |
| title | Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos. |
| title_full | Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos. |
| title_fullStr | Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos. |
| title_full_unstemmed | Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos. |
| title_short | Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos. |
| title_sort | genome wide association study of red blood cell traits in hispanics latinos the hispanic community health study study of latinos |
| url | https://doi.org/10.1371/journal.pgen.1006760 |
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