Development of the PCR Assay with Universal Primers for the Detection of Different <i>tcpA</i> Gene Variants
Developed is the PCR assay for the detection of the structural genes of toxin co-regulated adhesion piluses - tcpA of different types. Determined are the universal primers, the usage of which provides for the detection of the stated above genes in V. cholerae of various serogroups. With the help of...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2012-02-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/783 |
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| author | A. V. Fadeeva G. A. Eroshenko G. N. Odinokov N. A. Sharapova T. V. Valova V. V. Kutyrev |
| author_facet | A. V. Fadeeva G. A. Eroshenko G. N. Odinokov N. A. Sharapova T. V. Valova V. V. Kutyrev |
| author_sort | A. V. Fadeeva |
| collection | DOAJ |
| description | Developed is the PCR assay for the detection of the structural genes of toxin co-regulated adhesion piluses - tcpA of different types. Determined are the universal primers, the usage of which provides for the detection of the stated above genes in V. cholerae of various serogroups. With the help of this PCR assay identified is a new variant of tcpA gene in toxigenic cholera vibrio of non-O1/non-O139 serogroup. |
| format | Article |
| id | doaj-art-d777d06c639c408a80be5ecaecde1e02 |
| institution | Matheson Library |
| issn | 0370-1069 2658-719X |
| language | Russian |
| publishDate | 2012-02-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj-art-d777d06c639c408a80be5ecaecde1e022025-07-07T08:02:35ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2012-02-0101(111)10210310.21055/0370-1069-2012-1(111)-102-103767Development of the PCR Assay with Universal Primers for the Detection of Different <i>tcpA</i> Gene VariantsA. V. Fadeeva0G. A. Eroshenko1G. N. Odinokov2N. A. Sharapova3T. V. Valova4V. V. Kutyrev5Russian Research Anti-Plague Institute MicrobeRussian Research Anti-Plague Institute MicrobeRussian Research Anti-Plague Institute MicrobeRussian Research Anti-Plague Institute MicrobeRussian Research Anti-Plague Institute MicrobeRussian Research Anti-Plague Institute MicrobeDeveloped is the PCR assay for the detection of the structural genes of toxin co-regulated adhesion piluses - tcpA of different types. Determined are the universal primers, the usage of which provides for the detection of the stated above genes in V. cholerae of various serogroups. With the help of this PCR assay identified is a new variant of tcpA gene in toxigenic cholera vibrio of non-O1/non-O139 serogroup.https://journal.microbe.ru/jour/article/view/783cholera agentpathogenicity genepcr detection |
| spellingShingle | A. V. Fadeeva G. A. Eroshenko G. N. Odinokov N. A. Sharapova T. V. Valova V. V. Kutyrev Development of the PCR Assay with Universal Primers for the Detection of Different <i>tcpA</i> Gene Variants Проблемы особо опасных инфекций cholera agent pathogenicity gene pcr detection |
| title | Development of the PCR Assay with Universal Primers for the Detection of Different <i>tcpA</i> Gene Variants |
| title_full | Development of the PCR Assay with Universal Primers for the Detection of Different <i>tcpA</i> Gene Variants |
| title_fullStr | Development of the PCR Assay with Universal Primers for the Detection of Different <i>tcpA</i> Gene Variants |
| title_full_unstemmed | Development of the PCR Assay with Universal Primers for the Detection of Different <i>tcpA</i> Gene Variants |
| title_short | Development of the PCR Assay with Universal Primers for the Detection of Different <i>tcpA</i> Gene Variants |
| title_sort | development of the pcr assay with universal primers for the detection of different i tcpa i gene variants |
| topic | cholera agent pathogenicity gene pcr detection |
| url | https://journal.microbe.ru/jour/article/view/783 |
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