A sensitive SYBR Green RT-qPCR method for grapevine virus E and its application for virus detection in different grapevine sample types

To develop a rapid and high-sensitivity method for detection of grapevine virus E (GVE), a SYBR Green based real-time fluorescence quantitative RT-PCR method (RT-qPCR) was established. This method could be used to detect GVE specifically, and the sensitivity was about 100 times greater than conventi...

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Main Authors: Fang REN, Zun-ping ZHANG, Xu-dong FAN, Guo-jun HU, Meng-yan ZHANG, Ya-feng DONG
פורמט: Article
שפה:אנגלית
יצא לאור: KeAi Communications Co., Ltd. 2020-07-01
סדרה:Journal of Integrative Agriculture
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גישה מקוונת:http://www.sciencedirect.com/science/article/pii/S209531191962784X
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