Off-target interactions in the CRISPR-Cas9 Machinery: mechanisms and outcomes

Off-target interactions in the CRISPR-Cas9 Machinery: mechanisms and outcomes

The in vivo editing of genetic information necessitates tools of unprecedented accuracy. CRISPR-Cas-based systems have emerged as leading technologies for precisely targeting the genome. The Cas9 endonuclease derived from Streptococcus pyogenes is the most commonly used instrument for targeted DNA c...

Full description

Saved in:
Bibliographic Details
Main Authors: Lyubov Yu. Kanazhevskaya, Polina V. Zhdanova, Alexander A. Chernonosov, Vladimir V. Koval
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Biochemistry and Biophysics Reports
Subjects:
CRISPR
Cas9
Genome editing
sgRNA
Mismatch tolerance
Off-target activity
Online Access:http://www.sciencedirect.com/science/article/pii/S2405580825002213
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items

Similar Items