Genetic variability of the pear lace bug Stephanitis pyri F. (Heteroptera: Tingidae) in Krasnodar Territory, Russia
Aim. Study of the genetic variability of the pear lace bug Stephanitis pyri F. depending on geographical location and trophic specialization.Material and Methods. The object of the study was insect samples (n=60) from the natural population of the pear lace bug Stephanitis pyri F. (Tingidae: Heterop...
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Kamerton
2023-11-01
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| Series: | Юг России: экология, развитие |
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| Online Access: | https://ecodag.elpub.ru/ugro/article/view/2904 |
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| author | E. N. Besedinа V. I. Kil |
| author_facet | E. N. Besedinа V. I. Kil |
| author_sort | E. N. Besedinа |
| collection | DOAJ |
| description | Aim. Study of the genetic variability of the pear lace bug Stephanitis pyri F. depending on geographical location and trophic specialization.Material and Methods. The object of the study was insect samples (n=60) from the natural population of the pear lace bug Stephanitis pyri F. (Tingidae: Heteroptera) in the Krasnodar Territory: in Krasnodar, Kropotkin and Novorossiysk. The collection of S. pyri bugs was carried out on model trees of domestic apple (Malus domestica Borkh) and sour cherry (Prunus cerasus L.). Molecular genetic analysis was carried out in several stages: sample preparation, DNA extraction, and diagnostics based on RAPD (random amplified polymorphic DNA)‐PCR (DNA amplification).Results. With the help of molecular genetic markers, the genetic variability of the pear lace bug in the Krasnodar Territory was studied. The indicators of genetic differentiation between geographic (Gst=0.209) and trophic (Gst=0.049–0.302) intrapopulation groups of S. pyri were calculated. The levels of variability were determined within geographic (79.1 %) and trophic groups (70.0–95.1 %), as well as between them – 20.9 % and 4.9–30 %, respectively. The influence of the food factor on the genetic diversity and differentiation of insect populations is shown through the example of the Kropotkin trophic group of bugs that prefer the sour cherry P. cerasus as the main food plant.Conclusion. A small effect of isolation by distance was noted, which was characterized by a weak dependence of geographical and genetic distances between the population groups of the pear lace bug. The influence of the nutritional factor on the genetic variability of phytophages populations has been established, as is confirmed by other researchers. |
| format | Article |
| id | doaj-art-d6b1869fb8814e25b4517f2b39c4bf6d |
| institution | Matheson Library |
| issn | 1992-1098 2413-0958 |
| language | Russian |
| publishDate | 2023-11-01 |
| publisher | Kamerton |
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| series | Юг России: экология, развитие |
| spelling | doaj-art-d6b1869fb8814e25b4517f2b39c4bf6d2025-08-04T10:38:46ZrusKamertonЮг России: экология, развитие1992-10982413-09582023-11-01183283810.18470/1992-1098-2023-3-28-381319Genetic variability of the pear lace bug Stephanitis pyri F. (Heteroptera: Tingidae) in Krasnodar Territory, RussiaE. N. Besedinа0V. I. Kil1Federal Research Centre of Biological Plant ProtectionKuban State UniversityAim. Study of the genetic variability of the pear lace bug Stephanitis pyri F. depending on geographical location and trophic specialization.Material and Methods. The object of the study was insect samples (n=60) from the natural population of the pear lace bug Stephanitis pyri F. (Tingidae: Heteroptera) in the Krasnodar Territory: in Krasnodar, Kropotkin and Novorossiysk. The collection of S. pyri bugs was carried out on model trees of domestic apple (Malus domestica Borkh) and sour cherry (Prunus cerasus L.). Molecular genetic analysis was carried out in several stages: sample preparation, DNA extraction, and diagnostics based on RAPD (random amplified polymorphic DNA)‐PCR (DNA amplification).Results. With the help of molecular genetic markers, the genetic variability of the pear lace bug in the Krasnodar Territory was studied. The indicators of genetic differentiation between geographic (Gst=0.209) and trophic (Gst=0.049–0.302) intrapopulation groups of S. pyri were calculated. The levels of variability were determined within geographic (79.1 %) and trophic groups (70.0–95.1 %), as well as between them – 20.9 % and 4.9–30 %, respectively. The influence of the food factor on the genetic diversity and differentiation of insect populations is shown through the example of the Kropotkin trophic group of bugs that prefer the sour cherry P. cerasus as the main food plant.Conclusion. A small effect of isolation by distance was noted, which was characterized by a weak dependence of geographical and genetic distances between the population groups of the pear lace bug. The influence of the nutritional factor on the genetic variability of phytophages populations has been established, as is confirmed by other researchers.https://ecodag.elpub.ru/ugro/article/view/2904insectspear lace bug stephanitis pyri f.trophic specializationpcrgenetic diversitygenetic differentiation |
| spellingShingle | E. N. Besedinа V. I. Kil Genetic variability of the pear lace bug Stephanitis pyri F. (Heteroptera: Tingidae) in Krasnodar Territory, Russia Юг России: экология, развитие insects pear lace bug stephanitis pyri f. trophic specialization pcr genetic diversity genetic differentiation |
| title | Genetic variability of the pear lace bug Stephanitis pyri F. (Heteroptera: Tingidae) in Krasnodar Territory, Russia |
| title_full | Genetic variability of the pear lace bug Stephanitis pyri F. (Heteroptera: Tingidae) in Krasnodar Territory, Russia |
| title_fullStr | Genetic variability of the pear lace bug Stephanitis pyri F. (Heteroptera: Tingidae) in Krasnodar Territory, Russia |
| title_full_unstemmed | Genetic variability of the pear lace bug Stephanitis pyri F. (Heteroptera: Tingidae) in Krasnodar Territory, Russia |
| title_short | Genetic variability of the pear lace bug Stephanitis pyri F. (Heteroptera: Tingidae) in Krasnodar Territory, Russia |
| title_sort | genetic variability of the pear lace bug stephanitis pyri f heteroptera tingidae in krasnodar territory russia |
| topic | insects pear lace bug stephanitis pyri f. trophic specialization pcr genetic diversity genetic differentiation |
| url | https://ecodag.elpub.ru/ugro/article/view/2904 |
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