Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b
The proteasome beta-4 subunit is required for the assembly of 20S proteasome complex, forming a pivotal component for the ubiquitin–proteasome system. Emerging evidence indicates that proteasome beta-4 subunit may be involved in underlying progression and mechanisms of malignancies. However, the rol...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-06-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317705767 |
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| _version_ | 1839596708923703296 |
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| author | Xiaodong Zhang Di Lin Yueqin Lin Hongqing Chen Minghua Zou Shan Zhong Xuefeng Yi Siqi Han |
| author_facet | Xiaodong Zhang Di Lin Yueqin Lin Hongqing Chen Minghua Zou Shan Zhong Xuefeng Yi Siqi Han |
| author_sort | Xiaodong Zhang |
| collection | DOAJ |
| description | The proteasome beta-4 subunit is required for the assembly of 20S proteasome complex, forming a pivotal component for the ubiquitin–proteasome system. Emerging evidence indicates that proteasome beta-4 subunit may be involved in underlying progression and mechanisms of malignancies. However, the role of proteasome beta-4 subunit in melanoma is currently unknown. Here, we reported that proteasome beta-4 subunit was markedly upregulated in human melanoma tissues and cells, compared with normal skin samples. High proteasome beta-4 subunit levels were significantly associated with poor overall survival in patients with melanoma. Proteasome beta-4 subunit knockdown strongly decreased melanoma cell growth in vitro and in vivo. We further identified miR-148b as a negative regulator of proteasome beta-4 subunit. Enforced expression of miR-148b resulted in vitro growth inhibition of melanoma cells, whereas this inhibition was further abolished by enforced expression of proteasome beta-4 subunit. Our findings, for the first time, indicated that the miR-148b/proteasome beta-4 subunit axis contributed to the development of melanoma, revealing novel therapeutic targets for the treatment of melanoma. |
| format | Article |
| id | doaj-art-d6ad2a2fa9c9477bbcd2338a8ef8f79f |
| institution | Matheson Library |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-d6ad2a2fa9c9477bbcd2338a8ef8f79f2025-08-02T20:33:35ZengSAGE PublishingTumor Biology1423-03802017-06-013910.1177/1010428317705767Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148bXiaodong Zhang0Di Lin1Yueqin Lin2Hongqing Chen3Minghua Zou4Shan Zhong5Xuefeng Yi6Siqi Han7Department of Dermatology, Guangdong Provincial Si’an Hospital, Dongguan, ChinaDepartment of Dermatology, Guangdong Provincial Si’an Hospital, Dongguan, ChinaDepartment of Dermatology, Guangdong Provincial Si’an Hospital, Dongguan, ChinaDepartment of Dermatology, Guangdong Provincial Si’an Hospital, Dongguan, ChinaDepartment of Dermatology, Guangdong Provincial Si’an Hospital, Dongguan, ChinaDepartment of Dermatology, Guangdong Provincial Si’an Hospital, Dongguan, ChinaDepartment of Dermatology, Guangdong Provincial Si’an Hospital, Dongguan, ChinaDepartment of Medical Oncology, Jinling Hospital, Nanjing, ChinaThe proteasome beta-4 subunit is required for the assembly of 20S proteasome complex, forming a pivotal component for the ubiquitin–proteasome system. Emerging evidence indicates that proteasome beta-4 subunit may be involved in underlying progression and mechanisms of malignancies. However, the role of proteasome beta-4 subunit in melanoma is currently unknown. Here, we reported that proteasome beta-4 subunit was markedly upregulated in human melanoma tissues and cells, compared with normal skin samples. High proteasome beta-4 subunit levels were significantly associated with poor overall survival in patients with melanoma. Proteasome beta-4 subunit knockdown strongly decreased melanoma cell growth in vitro and in vivo. We further identified miR-148b as a negative regulator of proteasome beta-4 subunit. Enforced expression of miR-148b resulted in vitro growth inhibition of melanoma cells, whereas this inhibition was further abolished by enforced expression of proteasome beta-4 subunit. Our findings, for the first time, indicated that the miR-148b/proteasome beta-4 subunit axis contributed to the development of melanoma, revealing novel therapeutic targets for the treatment of melanoma.https://doi.org/10.1177/1010428317705767 |
| spellingShingle | Xiaodong Zhang Di Lin Yueqin Lin Hongqing Chen Minghua Zou Shan Zhong Xuefeng Yi Siqi Han Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b Tumor Biology |
| title | Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b |
| title_full | Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b |
| title_fullStr | Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b |
| title_full_unstemmed | Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b |
| title_short | Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b |
| title_sort | proteasome beta 4 subunit contributes to the development of melanoma and is regulated by mir 148b |
| url | https://doi.org/10.1177/1010428317705767 |
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