Preparation, Structural Characterization and in Vitro Antioxidant Activity of Selenized Polygonatum sibiricum Polysaccharides
Selenized Polygonatum sibiricum polysaccharides (PSP-Se) were prepared using a redox system containing glacial acetic acid and sodium selenite and characterized inductively coupled plasma atomic emission spectrometry (ICP-AES), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared...
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China Food Publishing Company
2025-06-01
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-11-001.pdf |
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| author | ZHAO Xinyue, WANG Qingyu, WANG Baixuan, ZHAO Qingxu, LI Dapeng, YANG Yue |
| author_facet | ZHAO Xinyue, WANG Qingyu, WANG Baixuan, ZHAO Qingxu, LI Dapeng, YANG Yue |
| author_sort | ZHAO Xinyue, WANG Qingyu, WANG Baixuan, ZHAO Qingxu, LI Dapeng, YANG Yue |
| collection | DOAJ |
| description | Selenized Polygonatum sibiricum polysaccharides (PSP-Se) were prepared using a redox system containing glacial acetic acid and sodium selenite and characterized inductively coupled plasma atomic emission spectrometry (ICP-AES), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), particle size and zeta potential analysis. The antioxidant activity of Polygonatum sibiricum polysaccharides (PSP) and PSP-Se were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2’-diazide-bis (3-ethyl-benzothiazole-6-sulfonic acid) (ABTS) cation radical assay and hydroxyl radical assays. The results showed that the optimal conditions for the preparation of PSP-Se were determined by response surface methodology (RSM) as follows: 1.5 g of sodium selenite and 3.0 mL of glacial acetic acid per g of PSP, 80 ℃ and 12 h. The as-prepared PSP-Se contained (6.00 ± 0.20) mg/g of Se, exhibited a spherical morphology with a particle size of 1 654 nm, and showed improved thermal stability and a 95.12% increase in the absolute value of the zeta potential compared with PSP. After selenium modification, the basic skeleton of PSP was preserved. Monosaccharide composition analysis showed that PSP-Se but not PSP contained N-acetyl-glucosamine and N-acetyl-galactosamine, with differences in the contents of shared monosaccharide components being observed between them. FTIR spectroscopy showed that two new absorption peaks appeared at 1 131 and 900 cm-1 in PSP-Se, indicating the formation of selenite ester bonds and successful selenization. In vitro antioxidant assays demonstrated that PSP-Se had significantly higher scavenging capacity against DPPH, ABTS cation and hydroxyl radical compared with PSP (P < 0.05), in a dose-dependent manner. |
| format | Article |
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| institution | Matheson Library |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | China Food Publishing Company |
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| series | Shipin Kexue |
| spelling | doaj-art-d5c3d9364a07453e9aa8d7ac6dda6ad62025-07-21T02:25:05ZengChina Food Publishing CompanyShipin Kexue1002-66302025-06-01461111010.7506/spkx1002-6630-20241116-128Preparation, Structural Characterization and in Vitro Antioxidant Activity of Selenized Polygonatum sibiricum PolysaccharidesZHAO Xinyue, WANG Qingyu, WANG Baixuan, ZHAO Qingxu, LI Dapeng, YANG Yue0(Key Laboratory of Food Nutrition and Human Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China)Selenized Polygonatum sibiricum polysaccharides (PSP-Se) were prepared using a redox system containing glacial acetic acid and sodium selenite and characterized inductively coupled plasma atomic emission spectrometry (ICP-AES), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), particle size and zeta potential analysis. The antioxidant activity of Polygonatum sibiricum polysaccharides (PSP) and PSP-Se were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2’-diazide-bis (3-ethyl-benzothiazole-6-sulfonic acid) (ABTS) cation radical assay and hydroxyl radical assays. The results showed that the optimal conditions for the preparation of PSP-Se were determined by response surface methodology (RSM) as follows: 1.5 g of sodium selenite and 3.0 mL of glacial acetic acid per g of PSP, 80 ℃ and 12 h. The as-prepared PSP-Se contained (6.00 ± 0.20) mg/g of Se, exhibited a spherical morphology with a particle size of 1 654 nm, and showed improved thermal stability and a 95.12% increase in the absolute value of the zeta potential compared with PSP. After selenium modification, the basic skeleton of PSP was preserved. Monosaccharide composition analysis showed that PSP-Se but not PSP contained N-acetyl-glucosamine and N-acetyl-galactosamine, with differences in the contents of shared monosaccharide components being observed between them. FTIR spectroscopy showed that two new absorption peaks appeared at 1 131 and 900 cm-1 in PSP-Se, indicating the formation of selenite ester bonds and successful selenization. In vitro antioxidant assays demonstrated that PSP-Se had significantly higher scavenging capacity against DPPH, ABTS cation and hydroxyl radical compared with PSP (P < 0.05), in a dose-dependent manner.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-11-001.pdfpolygonatum sibiricum polysaccharide; selenization modification; structural characterization; antioxidant activity |
| spellingShingle | ZHAO Xinyue, WANG Qingyu, WANG Baixuan, ZHAO Qingxu, LI Dapeng, YANG Yue Preparation, Structural Characterization and in Vitro Antioxidant Activity of Selenized Polygonatum sibiricum Polysaccharides Shipin Kexue polygonatum sibiricum polysaccharide; selenization modification; structural characterization; antioxidant activity |
| title | Preparation, Structural Characterization and in Vitro Antioxidant Activity of Selenized Polygonatum sibiricum Polysaccharides |
| title_full | Preparation, Structural Characterization and in Vitro Antioxidant Activity of Selenized Polygonatum sibiricum Polysaccharides |
| title_fullStr | Preparation, Structural Characterization and in Vitro Antioxidant Activity of Selenized Polygonatum sibiricum Polysaccharides |
| title_full_unstemmed | Preparation, Structural Characterization and in Vitro Antioxidant Activity of Selenized Polygonatum sibiricum Polysaccharides |
| title_short | Preparation, Structural Characterization and in Vitro Antioxidant Activity of Selenized Polygonatum sibiricum Polysaccharides |
| title_sort | preparation structural characterization and in vitro antioxidant activity of selenized polygonatum sibiricum polysaccharides |
| topic | polygonatum sibiricum polysaccharide; selenization modification; structural characterization; antioxidant activity |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-11-001.pdf |
| work_keys_str_mv | AT zhaoxinyuewangqingyuwangbaixuanzhaoqingxulidapengyangyue preparationstructuralcharacterizationandinvitroantioxidantactivityofselenizedpolygonatumsibiricumpolysaccharides |