Studies on the induction of cyanide-resistant respiration by H<sub>2</sub>O<sub>2</sub> in aging potato tuber slices
The influences of exogenous H<sub>2</sub>O<sub>2</sub> on the cyanide resistant respiration in aging potato (Solanum tuberosum L.) tuber slices were investigated. Results showed that H<sub>2</sub>O<sub>2</sub> (5.0 mmol/L) had a little influence on the...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2002-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2002.05.0525 |
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| Summary: | The influences of exogenous H<sub>2</sub>O<sub>2</sub> on the cyanide resistant respiration in aging potato (Solanum tuberosum L.) tuber slices were investigated. Results showed that H<sub>2</sub>O<sub>2</sub> (5.0 mmol/L) had a little influence on the total respiration (V<sub>t</sub>) of the slices, but showed a significant inducing effect on the cyanide resistant respiration (V<sub>alt</sub>) and its contribution to total respiration (V<sub>alt</sub>/V<sub>t</sub>) in the slices during 21 h of aging process. The significant inducing effects of H<sub>2</sub>O<sub>2</sub> on the cyanide-resistant respiration were also found in mitochondria purified from the aging potato slices. Western blotting with monoclonal antibody against alternative oxidase showed that H<sub>2</sub>O<sub>2</sub> treatment increased the expression of alternative oxidase in aging potato tuber slices, which implies that the induction of cyanide resistant respiration by H<sub>2</sub>O<sub>2</sub> was related to alternative oxidase expression. |
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| ISSN: | 1008-9209 2097-5155 |