Microbiota dynamic communities in sweet orange infected by “huanglongbing” in Iran
“Huanglongbing”-(HLB) or citrus-greening is one of the most serious citrus diseases worldwide. This study aimed to investigate the bacterial-communities associated with HLB-symptomatic sweet orange trees (Citrus sinensis) from different geographical regions in southern Iran. The 16S rRNA gene amplic...
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Firenze University Press
2025-05-01
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| Series: | Phytopathologia Mediterranea |
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| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/15662 |
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| author | Shiva SAFARPOUR KAPOURCHALI Mojdeh MALEKI Ali ALIZADEH ALIABADI Saeideh RAJAEE Mohammad MEHDI FAGHIHI Mehdi NASR ESFAHANI |
| author_facet | Shiva SAFARPOUR KAPOURCHALI Mojdeh MALEKI Ali ALIZADEH ALIABADI Saeideh RAJAEE Mohammad MEHDI FAGHIHI Mehdi NASR ESFAHANI |
| author_sort | Shiva SAFARPOUR KAPOURCHALI |
| collection | DOAJ |
| description | “Huanglongbing”-(HLB) or citrus-greening is one of the most serious citrus diseases worldwide. This study aimed to investigate the bacterial-communities associated with HLB-symptomatic sweet orange trees (Citrus sinensis) from different geographical regions in southern Iran. The 16S rRNA gene amplicon metagenomics sequencing of DNA extracted from the midrib and petiole tissues of symptomatic plants confirmed that the ‘Candidatus Liberibacter asiaticus’, was spread along the citrus-plantation regions in southern Iran, including Kerman, Sistan and Baluchistan, Fars, Hormozgan, and Khuzestan Provinces. The frequency of Operational Taxonomic Units (OTUs) related to ‘Ca. L. asiaticus’ was remarkable in the HLB symptomatic tree in the Fars region. No OTUs of ‘Ca. Liberibacter’ or ‘Ca. Phytoplasma’ were detected in the asymptomatic samples in the Kerman region. However, in asymptomatic materials representatives of the class Bacilli, including Lactobacillus spp. and Bacillus spp., showed 12- and 4-fold presence compared to the symptomatic samples of Kerman groves. Furthermore, the presence of OTUs belonging to ‘Ca. L. europaeus’ and ‘Ca. Phytoplasma aurantifolia’ was detected in sweet oranges. The simultaneous occurrence of ‘Ca. L. asiaticus’, ‘Ca. L. europaeus’, and ‘Ca. P. aurantifolia’ in HLB symptomatic orange trees in the Fars groves provided worthwhile insights for further research, although their epidemiological role in co-infections remains unknown. Microbial dataset in relation to variables associated with the plant health, defense, and disease helps to understand how these variables shape the citrus microbial community and identify individual that play a role in HLB suppression or promotion. |
| format | Article |
| id | doaj-art-d57aa6d5773743f980917b1e43f6f765 |
| institution | Matheson Library |
| issn | 0031-9465 1593-2095 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Firenze University Press |
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| series | Phytopathologia Mediterranea |
| spelling | doaj-art-d57aa6d5773743f980917b1e43f6f7652025-07-22T10:40:10ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952025-05-0164112914310.36253/phyto-1566214499Microbiota dynamic communities in sweet orange infected by “huanglongbing” in IranShiva SAFARPOUR KAPOURCHALI0https://orcid.org/0009-0006-6434-5608Mojdeh MALEKI1https://orcid.org/0000-0001-5355-8049Ali ALIZADEH ALIABADI2https://orcid.org/0000-0003-0636-7674Saeideh RAJAEE3Mohammad MEHDI FAGHIHI4Mehdi NASR ESFAHANI5Department of Plant Protection, Islamic Azad University, Varamin, Pishva Branch, Varamin, IranDepartment of Plant Pathology, Islamic Azad University, Varamin, Pishva Branch, Varamin, IranIranian Research Institute of Plant Protection, AREEO, Tehran, IranThe National Institute of Genetic Engineering and Biotechnology, NIGEB, Tehran, IranPlant Protection Research Department, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Zarghan, IranPlant Protection Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, AREEO, Isfahan, Iran“Huanglongbing”-(HLB) or citrus-greening is one of the most serious citrus diseases worldwide. This study aimed to investigate the bacterial-communities associated with HLB-symptomatic sweet orange trees (Citrus sinensis) from different geographical regions in southern Iran. The 16S rRNA gene amplicon metagenomics sequencing of DNA extracted from the midrib and petiole tissues of symptomatic plants confirmed that the ‘Candidatus Liberibacter asiaticus’, was spread along the citrus-plantation regions in southern Iran, including Kerman, Sistan and Baluchistan, Fars, Hormozgan, and Khuzestan Provinces. The frequency of Operational Taxonomic Units (OTUs) related to ‘Ca. L. asiaticus’ was remarkable in the HLB symptomatic tree in the Fars region. No OTUs of ‘Ca. Liberibacter’ or ‘Ca. Phytoplasma’ were detected in the asymptomatic samples in the Kerman region. However, in asymptomatic materials representatives of the class Bacilli, including Lactobacillus spp. and Bacillus spp., showed 12- and 4-fold presence compared to the symptomatic samples of Kerman groves. Furthermore, the presence of OTUs belonging to ‘Ca. L. europaeus’ and ‘Ca. Phytoplasma aurantifolia’ was detected in sweet oranges. The simultaneous occurrence of ‘Ca. L. asiaticus’, ‘Ca. L. europaeus’, and ‘Ca. P. aurantifolia’ in HLB symptomatic orange trees in the Fars groves provided worthwhile insights for further research, although their epidemiological role in co-infections remains unknown. Microbial dataset in relation to variables associated with the plant health, defense, and disease helps to understand how these variables shape the citrus microbial community and identify individual that play a role in HLB suppression or promotion.https://oajournals.fupress.net/index.php/pm/article/view/15662 ‘candidatus liberibacter’ speciesplant microbiotavalencia sweet orange |
| spellingShingle | Shiva SAFARPOUR KAPOURCHALI Mojdeh MALEKI Ali ALIZADEH ALIABADI Saeideh RAJAEE Mohammad MEHDI FAGHIHI Mehdi NASR ESFAHANI Microbiota dynamic communities in sweet orange infected by “huanglongbing” in Iran Phytopathologia Mediterranea ‘candidatus liberibacter’ species plant microbiota valencia sweet orange |
| title | Microbiota dynamic communities in sweet orange infected by “huanglongbing” in Iran |
| title_full | Microbiota dynamic communities in sweet orange infected by “huanglongbing” in Iran |
| title_fullStr | Microbiota dynamic communities in sweet orange infected by “huanglongbing” in Iran |
| title_full_unstemmed | Microbiota dynamic communities in sweet orange infected by “huanglongbing” in Iran |
| title_short | Microbiota dynamic communities in sweet orange infected by “huanglongbing” in Iran |
| title_sort | microbiota dynamic communities in sweet orange infected by huanglongbing in iran |
| topic | ‘candidatus liberibacter’ species plant microbiota valencia sweet orange |
| url | https://oajournals.fupress.net/index.php/pm/article/view/15662 |
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