Assisting monofloral honey classification by automated pollen identification based on convolutional neural networks

Categorising monoflorality of honey by human experts is labour intensive and time-consuming, resulting in the urgent need for automated techniques to overcome the constraints of traditional method which involves manually sorting of pollen grains. This study constructs a comprehensive dataset of poll...

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Main Authors: José Miguel Valiente, Juan José Martín-Osuna, Ana María Peral, Isabel Escriche
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:Ecological Informatics
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Online Access:http://www.sciencedirect.com/science/article/pii/S1574954125003498
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author José Miguel Valiente
Juan José Martín-Osuna
Ana María Peral
Isabel Escriche
author_facet José Miguel Valiente
Juan José Martín-Osuna
Ana María Peral
Isabel Escriche
author_sort José Miguel Valiente
collection DOAJ
description Categorising monoflorality of honey by human experts is labour intensive and time-consuming, resulting in the urgent need for automated techniques to overcome the constraints of traditional method which involves manually sorting of pollen grains. This study constructs a comprehensive dataset of pollen images, labelling them with an enhanced version of HoneyApp application. This Ground Truth termed POLLEN24_SP, comprises 32,285 pollen/particle images (captured by an expert using optical microscopy), covering the 24 most prevalent types of pollen grains found in Spanish honeys. Twelve different pre-existing Convolutional Neural Networks (CNN) were evaluated, achieving an accuracy rate of up to 98.03 % with EfficientNetV2M. The new version of in-house networks (PolleNetV2, PolleNetV2.mobile) was also introduced. The pre-existing CNN models' results were satisfactory in discerning the relative frequencies of different pollen types; nevertheless, our new proposals are promising due to their straightforward architecture as well as their scalability for integration into the HoneyApp ecosystem. Studying the classification errors made by the CNN models confirmed their strong generalization ability. To minimise errors in types such as Leguminoseae, segmenting this family is recommended. In addition, it is vital to continue expanding POLLEN24_SP dataset focusing on the underrepresented types to balance them and include more pollen types to better represent Spanish honeys. Finally, the results of this work will enable the creation of a public dataset to be diffused among the scientific community, following open science principles. The development of a computer application incorporating CNN models, makes these methodologies suitable for efficient classification of monofloral honeys.
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spelling doaj-art-d4a101267e7f44f3bb0d9f8c5f9180e72025-07-26T05:22:57ZengElsevierEcological Informatics1574-95412025-12-0190103340Assisting monofloral honey classification by automated pollen identification based on convolutional neural networksJosé Miguel Valiente0Juan José Martín-Osuna1Ana María Peral2Isabel Escriche3Institute of Control Systems and Industrial Computing (AI2), Universitat Politècnica de València, Camino de Vera. s/n. 46022, Valencia, Spain; Corresponding author.Institute of Control Systems and Industrial Computing (AI2), Universitat Politècnica de València, Camino de Vera. s/n. 46022, Valencia, SpainInstituto Universitario de Ingeniería de Alimentos (FoodUPV), Universitat Politècnica de València, Camino de Vera. s/n, 46022, Valencia, SpainInstituto Universitario de Ingeniería de Alimentos (FoodUPV), Universitat Politècnica de València, Camino de Vera. s/n, 46022, Valencia, Spain; Food Technology Department, Universitat Politècnica de València, Camino de Vera. s/n, 46022, Valencia, Spain; Corresponding author at: Instituto Universitario de Ingeniería de Alimentos (FoodUPV), Universitat Politècnica de València, Camino de Vera. s/n, 46022, Valencia, Spain.Categorising monoflorality of honey by human experts is labour intensive and time-consuming, resulting in the urgent need for automated techniques to overcome the constraints of traditional method which involves manually sorting of pollen grains. This study constructs a comprehensive dataset of pollen images, labelling them with an enhanced version of HoneyApp application. This Ground Truth termed POLLEN24_SP, comprises 32,285 pollen/particle images (captured by an expert using optical microscopy), covering the 24 most prevalent types of pollen grains found in Spanish honeys. Twelve different pre-existing Convolutional Neural Networks (CNN) were evaluated, achieving an accuracy rate of up to 98.03 % with EfficientNetV2M. The new version of in-house networks (PolleNetV2, PolleNetV2.mobile) was also introduced. The pre-existing CNN models' results were satisfactory in discerning the relative frequencies of different pollen types; nevertheless, our new proposals are promising due to their straightforward architecture as well as their scalability for integration into the HoneyApp ecosystem. Studying the classification errors made by the CNN models confirmed their strong generalization ability. To minimise errors in types such as Leguminoseae, segmenting this family is recommended. In addition, it is vital to continue expanding POLLEN24_SP dataset focusing on the underrepresented types to balance them and include more pollen types to better represent Spanish honeys. Finally, the results of this work will enable the creation of a public dataset to be diffused among the scientific community, following open science principles. The development of a computer application incorporating CNN models, makes these methodologies suitable for efficient classification of monofloral honeys.http://www.sciencedirect.com/science/article/pii/S1574954125003498Monofloral honey classificationPollen classificationPollen datasetLabelling and annotating applicationHoneyAppConvolutional neural networks
spellingShingle José Miguel Valiente
Juan José Martín-Osuna
Ana María Peral
Isabel Escriche
Assisting monofloral honey classification by automated pollen identification based on convolutional neural networks
Ecological Informatics
Monofloral honey classification
Pollen classification
Pollen dataset
Labelling and annotating application
HoneyApp
Convolutional neural networks
title Assisting monofloral honey classification by automated pollen identification based on convolutional neural networks
title_full Assisting monofloral honey classification by automated pollen identification based on convolutional neural networks
title_fullStr Assisting monofloral honey classification by automated pollen identification based on convolutional neural networks
title_full_unstemmed Assisting monofloral honey classification by automated pollen identification based on convolutional neural networks
title_short Assisting monofloral honey classification by automated pollen identification based on convolutional neural networks
title_sort assisting monofloral honey classification by automated pollen identification based on convolutional neural networks
topic Monofloral honey classification
Pollen classification
Pollen dataset
Labelling and annotating application
HoneyApp
Convolutional neural networks
url http://www.sciencedirect.com/science/article/pii/S1574954125003498
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