Effect of pasteurisation and high-pressure processing on selected bioactive components in human milk - An experimental study
High-pressure processing (HPP) represents a promising alternative to conventional Holder pasteurisation (HoP) used by human milk banks worldwide. The objective of this study was to identify whether the HPP would achieve the same or better retention of the content of selected analytes than the HoP. S...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2025-06-01
|
| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-202503-0008_effect-of-pasteurisation-and-high-pressure-processing-on-selected-bioactive-components-in-human-milk-8211-an.php |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839652285458677760 |
|---|---|
| author | Miroslava Jandová Michaela Fišerová Pavla Paterová Pavel Měřička Jan Malý Marián Kacerovský Eliška Kovaříková Jan Strohalm Kateřina Demnerová Jana Kadavá (email: jana.kadava@vs |
| author_facet | Miroslava Jandová Michaela Fišerová Pavla Paterová Pavel Měřička Jan Malý Marián Kacerovský Eliška Kovaříková Jan Strohalm Kateřina Demnerová Jana Kadavá (email: jana.kadava@vs |
| author_sort | Miroslava Jandová |
| collection | DOAJ |
| description | High-pressure processing (HPP) represents a promising alternative to conventional Holder pasteurisation (HoP) used by human milk banks worldwide. The objective of this study was to identify whether the HPP would achieve the same or better retention of the content of selected analytes than the HoP. Samples collected from 15 breast milk donors were processed in four ways: i) no treatment; ii) HoP; iii) HPP in cycles (350 MPa, 4 cycles); iv) continuous HPP (350 MPa, 20 min). The content of secretory immunoglobulin A (sIgA), lactoferrin and lysozyme was determined using commercially available ELISA kits, and the lipase activity was assessed using an A-lipase activity assay kit. Data were compared statistically using paired t-tests. HoP significantly reduced the content of lysozyme and lactoferrin as well as lipase activity (P < 0.001). Cycled HPP significantly decreased lipase activity (P = 0.002), while continuous HPP led to a significant decrease in lysozyme content (P = 0.001) and lipase activity (P = 0.014). Cycled HPP showed high retention of pretreatment levels of lysozyme - median 99 (88; 99%), lactoferrin - 84 (66; 105%), and sIgA content - 83 (28; 117%). Among the studied treatment regimens, the best preservation of initial levels of bioactive components was achieved using HPP at 350 MPa in cycles. |
| format | Article |
| id | doaj-art-d3e9d72ba65d45e6b4b41d4cea9b40b0 |
| institution | Matheson Library |
| issn | 1212-1800 1805-9317 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj-art-d3e9d72ba65d45e6b4b41d4cea9b40b02025-06-25T17:16:42ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172025-06-0143317017810.17221/45/2025-CJFScjf-202503-0008Effect of pasteurisation and high-pressure processing on selected bioactive components in human milk - An experimental studyMiroslava Jandová0https://orcid.org/0000-0001-5432-5458Michaela Fišerová1Pavla Paterová2https://orcid.org/0000-0003-0192-7345Pavel Měřička3https://orcid.org/0000-0002-8408-3645Jan Malý4Marián Kacerovský5https://orcid.org/0000-0001-9858-7900Eliška Kovaříková6https://orcid.org/0009-0005-2355-1686Jan Strohalm7Kateřina Demnerová8https://orcid.org/0000-0003-2050-4073Jana Kadavá (email: jana.kadava@vs9Tissue Bank, University Hospital Hradec Králové, Hradec Králové, Czech RepublicTissue Bank, University Hospital Hradec Králové, Hradec Králové, Czech RepublicDepartment of Clinical Microbiology, University Hospital and Faculty of Medicine in Hradec Králové, Charles University, Hradec Králové, Czech RepublicTissue Bank, University Hospital Hradec Králové, Hradec Králové, Czech RepublicDepartment of Pediatrics, University Hospital Hradec Králové, Hradec Králové, Czech RepublicBiomedical Research Center, University Hospital Hradec Králové, Hradec Králové, Czech RepublicFood Research Institute Prague, Prague, Czech RepublicFood Research Institute Prague, Prague, Czech RepublicDepartment of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Czech RepublicTissue Bank, University Hospital Hradec Králové, Hradec Králové, Czech RepublicHigh-pressure processing (HPP) represents a promising alternative to conventional Holder pasteurisation (HoP) used by human milk banks worldwide. The objective of this study was to identify whether the HPP would achieve the same or better retention of the content of selected analytes than the HoP. Samples collected from 15 breast milk donors were processed in four ways: i) no treatment; ii) HoP; iii) HPP in cycles (350 MPa, 4 cycles); iv) continuous HPP (350 MPa, 20 min). The content of secretory immunoglobulin A (sIgA), lactoferrin and lysozyme was determined using commercially available ELISA kits, and the lipase activity was assessed using an A-lipase activity assay kit. Data were compared statistically using paired t-tests. HoP significantly reduced the content of lysozyme and lactoferrin as well as lipase activity (P < 0.001). Cycled HPP significantly decreased lipase activity (P = 0.002), while continuous HPP led to a significant decrease in lysozyme content (P = 0.001) and lipase activity (P = 0.014). Cycled HPP showed high retention of pretreatment levels of lysozyme - median 99 (88; 99%), lactoferrin - 84 (66; 105%), and sIgA content - 83 (28; 117%). Among the studied treatment regimens, the best preservation of initial levels of bioactive components was achieved using HPP at 350 MPa in cycles.https://cjfs.agriculturejournals.cz/artkey/cjf-202503-0008_effect-of-pasteurisation-and-high-pressure-processing-on-selected-bioactive-components-in-human-milk-8211-an.phpbioactive componentsbreast milkhigh-pressure processingholder pasteurisationmilk bank |
| spellingShingle | Miroslava Jandová Michaela Fišerová Pavla Paterová Pavel Měřička Jan Malý Marián Kacerovský Eliška Kovaříková Jan Strohalm Kateřina Demnerová Jana Kadavá (email: jana.kadava@vs Effect of pasteurisation and high-pressure processing on selected bioactive components in human milk - An experimental study Czech Journal of Food Sciences bioactive components breast milk high-pressure processing holder pasteurisation milk bank |
| title | Effect of pasteurisation and high-pressure processing on selected bioactive components in human milk - An experimental study |
| title_full | Effect of pasteurisation and high-pressure processing on selected bioactive components in human milk - An experimental study |
| title_fullStr | Effect of pasteurisation and high-pressure processing on selected bioactive components in human milk - An experimental study |
| title_full_unstemmed | Effect of pasteurisation and high-pressure processing on selected bioactive components in human milk - An experimental study |
| title_short | Effect of pasteurisation and high-pressure processing on selected bioactive components in human milk - An experimental study |
| title_sort | effect of pasteurisation and high pressure processing on selected bioactive components in human milk an experimental study |
| topic | bioactive components breast milk high-pressure processing holder pasteurisation milk bank |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-202503-0008_effect-of-pasteurisation-and-high-pressure-processing-on-selected-bioactive-components-in-human-milk-8211-an.php |
| work_keys_str_mv | AT miroslavajandova effectofpasteurisationandhighpressureprocessingonselectedbioactivecomponentsinhumanmilkanexperimentalstudy AT michaelafiserova effectofpasteurisationandhighpressureprocessingonselectedbioactivecomponentsinhumanmilkanexperimentalstudy AT pavlapaterova effectofpasteurisationandhighpressureprocessingonselectedbioactivecomponentsinhumanmilkanexperimentalstudy AT pavelmericka effectofpasteurisationandhighpressureprocessingonselectedbioactivecomponentsinhumanmilkanexperimentalstudy AT janmaly effectofpasteurisationandhighpressureprocessingonselectedbioactivecomponentsinhumanmilkanexperimentalstudy AT mariankacerovsky effectofpasteurisationandhighpressureprocessingonselectedbioactivecomponentsinhumanmilkanexperimentalstudy AT eliskakovarikova effectofpasteurisationandhighpressureprocessingonselectedbioactivecomponentsinhumanmilkanexperimentalstudy AT janstrohalm effectofpasteurisationandhighpressureprocessingonselectedbioactivecomponentsinhumanmilkanexperimentalstudy AT katerinademnerova effectofpasteurisationandhighpressureprocessingonselectedbioactivecomponentsinhumanmilkanexperimentalstudy AT janakadavaemailjanakadavavs effectofpasteurisationandhighpressureprocessingonselectedbioactivecomponentsinhumanmilkanexperimentalstudy |