Promotive role of MBNL3 and PXN genes expressions with lncRNA PXN-AS1-L on gastric cancer
Background: Gastric cancer ranks fourth in both incidence and mortality worldwide. Several genes influence its genesis and progress. Materials and methods: The expression of MBNL3, PXN genes, and lncRNA PXN-AS1-L was evaluated in gastric tissue samples from 75 gastric cancer patients and 75 controls...
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Elsevier
2025-09-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580825002043 |
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| author | Mai A.H. Abouelenin Amany A. Saleh Naglaa S. Elabd Osama Elbahr Reham Ahmed Abdelaziz Mohammed G. Elhelbawy Zeinab A. Kasemy Ahmed Abdelkhalek Hussein Mona A. Abbas |
| author_facet | Mai A.H. Abouelenin Amany A. Saleh Naglaa S. Elabd Osama Elbahr Reham Ahmed Abdelaziz Mohammed G. Elhelbawy Zeinab A. Kasemy Ahmed Abdelkhalek Hussein Mona A. Abbas |
| author_sort | Mai A.H. Abouelenin |
| collection | DOAJ |
| description | Background: Gastric cancer ranks fourth in both incidence and mortality worldwide. Several genes influence its genesis and progress. Materials and methods: The expression of MBNL3, PXN genes, and lncRNA PXN-AS1-L was evaluated in gastric tissue samples from 75 gastric cancer patients and 75 controls by RT-PCR. Results: MBNL3, PXN, and lncRNA PXN-AS1-L expressions were considerably raised among cancerous gastric tissue than in nearby normal tissue and normal tissue from controls (p < 0.001). LncRNA PXN-AS1-L and MBNL3 had the highest sensitivity (92.00 % and 90.67 %, respectively), with PXN sensitivity of 81.33 % for differentiation of the cancer tissue from adjacent normal tissue, while PXN and lncRNA PXN-AS1-L had the highest sensitivity (98.67 % and 92.00 %, respectively), with 89.33 % for MBNL3 as discriminators between gastric cancer tissue and normal tissue from controls. Between metastatic and non-metastatic patients, MBNL3 and PXN were the highest differentiating markers (sensitivity = 87.50 % and 83.33 %, respectively). MBNL3, PXN, and lncRNA PXN-AS1-L were reliable predictors of gastric cancer (p = 0.004, p = 0.009, and p = 0.001, respectively), but only MBNL3 and lncRNA PXN-AS1-L were significant predictors for mortality (p = 0.026 and p = 0.043, respectively). The elevated expressions were linked to lower overall and progression-free survival rates. Conclusion: MBNL3, PXN, and lncRNA PXN-AS1-L expression levels can diagnose and predict gastric cancer and discriminate between metastatic and non-metastatic patients. The expression level of MBNL3 and lncRNA PXN-AS1-L can predict gastric cancer mortality. Higher expression of MBNL3 and PXN was linked to aggressive gastric cancer and low overall and progression-free survival rates. |
| format | Article |
| id | doaj-art-d3cba99d90694ab28f3e5edf8c5ec35d |
| institution | Matheson Library |
| issn | 2405-5808 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
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| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-d3cba99d90694ab28f3e5edf8c5ec35d2025-06-28T05:30:27ZengElsevierBiochemistry and Biophysics Reports2405-58082025-09-0143102117Promotive role of MBNL3 and PXN genes expressions with lncRNA PXN-AS1-L on gastric cancerMai A.H. Abouelenin0Amany A. Saleh1Naglaa S. Elabd2Osama Elbahr3Reham Ahmed Abdelaziz4Mohammed G. Elhelbawy5Zeinab A. Kasemy6Ahmed Abdelkhalek Hussein7Mona A. Abbas8Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Shebin El Kom, 32511, Egypt; Corresponding author.Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Shebin El Kom, 32511, Egypt; Medical Surgical Nursing Department, College of Nursing, Taibah University, KSA, Madina, Saudi ArabiaTropical Medicine Department, Faculty of Medicine, Menoufia University, Shebin El Kom, 32511, EgyptHepatology and Gastroenterology Department, National Liver Institute, Menoufia University, Shebin El Kom, 32511, EgyptClinical Oncology and Nuclear Medicine Department, Faculty of Medicine, Menoufia University, Shebin El Kom, 32511, EgyptClinical Pathology Department, Faculty of Medicine, Menoufia University, Shebin El Kom, 32511, EgyptPublic Health and Community Medicine Department, Faculty of Medicine, Menoufia University, Shebin El Kom, 32511, EgyptOncology Department, Radiation Oncology Section, King Faisal Specialist Hospital and Research Center (KFSHRC), KSA, P.O. Box 40047, Jeddah, 21499, Saudi ArabiaMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Shebin El Kom, 32511, EgyptBackground: Gastric cancer ranks fourth in both incidence and mortality worldwide. Several genes influence its genesis and progress. Materials and methods: The expression of MBNL3, PXN genes, and lncRNA PXN-AS1-L was evaluated in gastric tissue samples from 75 gastric cancer patients and 75 controls by RT-PCR. Results: MBNL3, PXN, and lncRNA PXN-AS1-L expressions were considerably raised among cancerous gastric tissue than in nearby normal tissue and normal tissue from controls (p < 0.001). LncRNA PXN-AS1-L and MBNL3 had the highest sensitivity (92.00 % and 90.67 %, respectively), with PXN sensitivity of 81.33 % for differentiation of the cancer tissue from adjacent normal tissue, while PXN and lncRNA PXN-AS1-L had the highest sensitivity (98.67 % and 92.00 %, respectively), with 89.33 % for MBNL3 as discriminators between gastric cancer tissue and normal tissue from controls. Between metastatic and non-metastatic patients, MBNL3 and PXN were the highest differentiating markers (sensitivity = 87.50 % and 83.33 %, respectively). MBNL3, PXN, and lncRNA PXN-AS1-L were reliable predictors of gastric cancer (p = 0.004, p = 0.009, and p = 0.001, respectively), but only MBNL3 and lncRNA PXN-AS1-L were significant predictors for mortality (p = 0.026 and p = 0.043, respectively). The elevated expressions were linked to lower overall and progression-free survival rates. Conclusion: MBNL3, PXN, and lncRNA PXN-AS1-L expression levels can diagnose and predict gastric cancer and discriminate between metastatic and non-metastatic patients. The expression level of MBNL3 and lncRNA PXN-AS1-L can predict gastric cancer mortality. Higher expression of MBNL3 and PXN was linked to aggressive gastric cancer and low overall and progression-free survival rates.http://www.sciencedirect.com/science/article/pii/S2405580825002043Alternative splicingExpressionGastric cancerlncRNA |
| spellingShingle | Mai A.H. Abouelenin Amany A. Saleh Naglaa S. Elabd Osama Elbahr Reham Ahmed Abdelaziz Mohammed G. Elhelbawy Zeinab A. Kasemy Ahmed Abdelkhalek Hussein Mona A. Abbas Promotive role of MBNL3 and PXN genes expressions with lncRNA PXN-AS1-L on gastric cancer Biochemistry and Biophysics Reports Alternative splicing Expression Gastric cancer lncRNA |
| title | Promotive role of MBNL3 and PXN genes expressions with lncRNA PXN-AS1-L on gastric cancer |
| title_full | Promotive role of MBNL3 and PXN genes expressions with lncRNA PXN-AS1-L on gastric cancer |
| title_fullStr | Promotive role of MBNL3 and PXN genes expressions with lncRNA PXN-AS1-L on gastric cancer |
| title_full_unstemmed | Promotive role of MBNL3 and PXN genes expressions with lncRNA PXN-AS1-L on gastric cancer |
| title_short | Promotive role of MBNL3 and PXN genes expressions with lncRNA PXN-AS1-L on gastric cancer |
| title_sort | promotive role of mbnl3 and pxn genes expressions with lncrna pxn as1 l on gastric cancer |
| topic | Alternative splicing Expression Gastric cancer lncRNA |
| url | http://www.sciencedirect.com/science/article/pii/S2405580825002043 |
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