Improved husbandry measures to enhance reproducibility of wound healing studies in the Leprdb/db mouse
Abstract The Leprdb/db mouse is a common and well‐studied model of type II diabetes mellitus that is often employed in biomedical research. Despite being one of the most commonly used models for the investigation of diabetic wound healing, there are a few specific guidelines for its husbandry, and w...
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| Format: | Article |
| Language: | English |
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Wiley
2025-06-01
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| Series: | Animal Models and Experimental Medicine |
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| Online Access: | https://doi.org/10.1002/ame2.70010 |
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| author | May Barakat Brian P. David Junhe Shi Angela Xu Terry W. Moore Lin Chen Luisa A. DiPietro |
| author_facet | May Barakat Brian P. David Junhe Shi Angela Xu Terry W. Moore Lin Chen Luisa A. DiPietro |
| author_sort | May Barakat |
| collection | DOAJ |
| description | Abstract The Leprdb/db mouse is a common and well‐studied model of type II diabetes mellitus that is often employed in biomedical research. Despite being one of the most commonly used models for the investigation of diabetic wound healing, there are a few specific guidelines for its husbandry, and wound complications such as infection and expansion are common. This study presents a modified animal husbandry approach for the Leprdb/db mouse to reduce the incidence of complications during wound healing experiments. Compared to standard rodent housing protocols, the use of this modified protocol leads to decreased rates of complications among experimental animals across several experiments. The protocol includes increased cage size, decreased housing density, and more frequent cage replacements. The use of improved husbandry for the Leprdb/db mouse decreases the total number of animals required, minimizes harm during experimentation, and improves the consistency and reproducibility of wound healing studies. |
| format | Article |
| id | doaj-art-d3847c9d0a8e4a1da8d6fa91c2562e31 |
| institution | Matheson Library |
| issn | 2576-2095 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Animal Models and Experimental Medicine |
| spelling | doaj-art-d3847c9d0a8e4a1da8d6fa91c2562e312025-06-28T04:48:14ZengWileyAnimal Models and Experimental Medicine2576-20952025-06-01861130113710.1002/ame2.70010Improved husbandry measures to enhance reproducibility of wound healing studies in the Leprdb/db mouseMay Barakat0Brian P. David1Junhe Shi2Angela Xu3Terry W. Moore4Lin Chen5Luisa A. DiPietro6Center for Wound Healing and Tissue Regeneration, College of Dentistry University of Illinois Chicago Chicago Illinois USADepartment of Pharmaceutical Sciences University of Illinois Chicago College of Pharmacy Chicago Illinois USACenter for Wound Healing and Tissue Regeneration, College of Dentistry University of Illinois Chicago Chicago Illinois USACenter for Wound Healing and Tissue Regeneration, College of Dentistry University of Illinois Chicago Chicago Illinois USADepartment of Pharmaceutical Sciences University of Illinois Chicago College of Pharmacy Chicago Illinois USACenter for Wound Healing and Tissue Regeneration, College of Dentistry University of Illinois Chicago Chicago Illinois USACenter for Wound Healing and Tissue Regeneration, College of Dentistry University of Illinois Chicago Chicago Illinois USAAbstract The Leprdb/db mouse is a common and well‐studied model of type II diabetes mellitus that is often employed in biomedical research. Despite being one of the most commonly used models for the investigation of diabetic wound healing, there are a few specific guidelines for its husbandry, and wound complications such as infection and expansion are common. This study presents a modified animal husbandry approach for the Leprdb/db mouse to reduce the incidence of complications during wound healing experiments. Compared to standard rodent housing protocols, the use of this modified protocol leads to decreased rates of complications among experimental animals across several experiments. The protocol includes increased cage size, decreased housing density, and more frequent cage replacements. The use of improved husbandry for the Leprdb/db mouse decreases the total number of animals required, minimizes harm during experimentation, and improves the consistency and reproducibility of wound healing studies.https://doi.org/10.1002/ame2.70010animal husbandryanimal models of diabetesdiabetic micemouse husbandrywound healing |
| spellingShingle | May Barakat Brian P. David Junhe Shi Angela Xu Terry W. Moore Lin Chen Luisa A. DiPietro Improved husbandry measures to enhance reproducibility of wound healing studies in the Leprdb/db mouse Animal Models and Experimental Medicine animal husbandry animal models of diabetes diabetic mice mouse husbandry wound healing |
| title | Improved husbandry measures to enhance reproducibility of wound healing studies in the Leprdb/db mouse |
| title_full | Improved husbandry measures to enhance reproducibility of wound healing studies in the Leprdb/db mouse |
| title_fullStr | Improved husbandry measures to enhance reproducibility of wound healing studies in the Leprdb/db mouse |
| title_full_unstemmed | Improved husbandry measures to enhance reproducibility of wound healing studies in the Leprdb/db mouse |
| title_short | Improved husbandry measures to enhance reproducibility of wound healing studies in the Leprdb/db mouse |
| title_sort | improved husbandry measures to enhance reproducibility of wound healing studies in the leprdb db mouse |
| topic | animal husbandry animal models of diabetes diabetic mice mouse husbandry wound healing |
| url | https://doi.org/10.1002/ame2.70010 |
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