GLAD-PCR assay of DNA methylation sites in regulatory regions of some tumor-suppressor genes in breast cancer
Hypermethylation of the RcgY sites is shown for many cancer diseases. such aberrant methylation, suppressing the gene activity, occurs at early stages of carcinogenesis. Recently, using glad-pcR assay, we have detected aberrantly methylated RcgY sites, which can be considered to be epigenetic marker...
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Russian Academy of Sciences, Tomsk National Research Medical Center
2022-01-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/1984 |
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| author | N. A. Smetannikova M. A. Abdurashitov A. G. Akishev P. I. Pozdnyakov E. V. Dubinin A. B. Karpov I. V. Vihlyanov M. K. Nikitin S. M. Soldatova N. A. Netesova |
| author_facet | N. A. Smetannikova M. A. Abdurashitov A. G. Akishev P. I. Pozdnyakov E. V. Dubinin A. B. Karpov I. V. Vihlyanov M. K. Nikitin S. M. Soldatova N. A. Netesova |
| author_sort | N. A. Smetannikova |
| collection | DOAJ |
| description | Hypermethylation of the RcgY sites is shown for many cancer diseases. such aberrant methylation, suppressing the gene activity, occurs at early stages of carcinogenesis. Recently, using glad-pcR assay, we have detected aberrantly methylated RcgY sites, which can be considered to be epigenetic markers of colorectal, lung, and gastric cancers. in breast cancer, methylation of the regulatory regions of ALX4, BMP2, CCND2, CDH13, CDX1, FOXA1, GALR1, GATA5, GREM1, HIC1, HMX2, HS3ST2, HOXC10, ICAM5, LAMA1, RARB, RASSF1A, RUNX3, RXRG, RYR2, SFRP2, SOX17, TERT, and ZNF613 tumor-suppressor genes is reported. in the present work, we determined aberrantly methylated RcgY sites in the regulatory regions of these genes in dNa preparations from breast cancer tissues. the study of dNa samples from 30 tumor and 22 normal mammary tissue samples demonstrates a high diagnostic potential of selected R(5mc)gY sites in regulatory regions of CCND2, BMP2, GALR1, SOX17, HMX2, and HS3ST2 genes with total index of sensitivity and specificity for R(5mc)gY detection in tumor dNa 90.0 % and 100.0 %, respectively. |
| format | Article |
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| institution | Matheson Library |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2022-01-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-d35e5e76ac3b4d3db32e8fffbd2f8b172025-08-04T10:33:41ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682022-01-01206415410.21294/1814-4861-2021-20-6-41-54923GLAD-PCR assay of DNA methylation sites in regulatory regions of some tumor-suppressor genes in breast cancerN. A. Smetannikova0M. A. Abdurashitov1A. G. Akishev2P. I. Pozdnyakov3E. V. Dubinin4A. B. Karpov5I. V. Vihlyanov6M. K. Nikitin7S. M. Soldatova8N. A. Netesova9State Research center of Virology and Biotechnology «Vector»Sibenzyme LtdSibenzyme LtdState Research center of Virology and Biotechnology «Vector»Epigene LtdSeversk Biophysical Research Centre; Siberian State Medical UniversityAltai Regional Oncology CenterAltai Regional Oncology CenterSiberian Federal Research And Clinical Centrestate Research center of Virology and Biotechnology «Vector»Hypermethylation of the RcgY sites is shown for many cancer diseases. such aberrant methylation, suppressing the gene activity, occurs at early stages of carcinogenesis. Recently, using glad-pcR assay, we have detected aberrantly methylated RcgY sites, which can be considered to be epigenetic markers of colorectal, lung, and gastric cancers. in breast cancer, methylation of the regulatory regions of ALX4, BMP2, CCND2, CDH13, CDX1, FOXA1, GALR1, GATA5, GREM1, HIC1, HMX2, HS3ST2, HOXC10, ICAM5, LAMA1, RARB, RASSF1A, RUNX3, RXRG, RYR2, SFRP2, SOX17, TERT, and ZNF613 tumor-suppressor genes is reported. in the present work, we determined aberrantly methylated RcgY sites in the regulatory regions of these genes in dNa preparations from breast cancer tissues. the study of dNa samples from 30 tumor and 22 normal mammary tissue samples demonstrates a high diagnostic potential of selected R(5mc)gY sites in regulatory regions of CCND2, BMP2, GALR1, SOX17, HMX2, and HS3ST2 genes with total index of sensitivity and specificity for R(5mc)gY detection in tumor dNa 90.0 % and 100.0 %, respectively.https://www.siboncoj.ru/jour/article/view/1984breast cancertumor-suppressor genesdna methylationglad-pcr assay |
| spellingShingle | N. A. Smetannikova M. A. Abdurashitov A. G. Akishev P. I. Pozdnyakov E. V. Dubinin A. B. Karpov I. V. Vihlyanov M. K. Nikitin S. M. Soldatova N. A. Netesova GLAD-PCR assay of DNA methylation sites in regulatory regions of some tumor-suppressor genes in breast cancer Сибирский онкологический журнал breast cancer tumor-suppressor genes dna methylation glad-pcr assay |
| title | GLAD-PCR assay of DNA methylation sites in regulatory regions of some tumor-suppressor genes in breast cancer |
| title_full | GLAD-PCR assay of DNA methylation sites in regulatory regions of some tumor-suppressor genes in breast cancer |
| title_fullStr | GLAD-PCR assay of DNA methylation sites in regulatory regions of some tumor-suppressor genes in breast cancer |
| title_full_unstemmed | GLAD-PCR assay of DNA methylation sites in regulatory regions of some tumor-suppressor genes in breast cancer |
| title_short | GLAD-PCR assay of DNA methylation sites in regulatory regions of some tumor-suppressor genes in breast cancer |
| title_sort | glad pcr assay of dna methylation sites in regulatory regions of some tumor suppressor genes in breast cancer |
| topic | breast cancer tumor-suppressor genes dna methylation glad-pcr assay |
| url | https://www.siboncoj.ru/jour/article/view/1984 |
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