Effect of storage medium of extracted human teeth on the result of in vitro studies – a scoping review
Purpose: This scoping review aimed to investigate how different storage media of extracted human teeth before and after preparation of specimens influence the outcomes of various dental materials intended for clinical application. Materials and methods: Literature search in four databases and by...
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| Language: | English |
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Medical Journals Sweden
2025-07-01
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| Series: | Biomaterial Investigations in Dentistry |
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| Online Access: | https://medicaljournalssweden.se/biid/article/view/44183 |
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| author | Muhammad Sadiq Billoo Muhammad Ahsan Kaleem Khan Khan Taimur Khalid Syed Murtaza Raza Kazmi |
| author_facet | Muhammad Sadiq Billoo Muhammad Ahsan Kaleem Khan Khan Taimur Khalid Syed Murtaza Raza Kazmi |
| author_sort | Muhammad Sadiq Billoo |
| collection | DOAJ |
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Purpose: This scoping review aimed to investigate how different storage media of extracted human teeth before and after preparation of specimens influence the outcomes of various dental materials intended for clinical application.
Materials and methods: Literature search in four databases and by manual searching was carried out on a predefined PIO as follows: Population – extracted human teeth; Intervention – different storage media; Outcome(s) – material properties.
Results: After screening 110 studies, 11 were included in the scoping review. Eight studies examined storage media effects before specimen preparation and three after specimen preparation. Four studies focused on enamel bonding, six on dentine, and one both on enamel and dentine. Concerning storage of extracted teeth prior to specimen preparation, cryopreservation, Chloramine T and thymol had no negative effect on bond strength to enamel. One study found Chloramine T to increase microleakage at enamel margins whereas another study found no negative effect. Storage in formalin, ethanol and thymol also had no negative effect on microleakage at enamel margins. Cryopreservation decreased bond strength to coronal dentine, but not to mid-coronal or deep dentine. Formalin, Chloramine T, ethanol and thymol had no negative effect on bond strength to dentine nor on microleakage at dentine margins. However, one study found long-term storage in Chloramine T to increase microleakage at dentine margins. Concerning storage of extracted teeth after specimen preparation, thymol significantly lowered bond strength to enamel, whereas formalin had no negative effect. Sodium hypochlorite had no negative effect on bond strength to dentine, and finally, formalin, Chloramine T, ethanol, and thymol had no negative effect on microleakage at dentine margins. Risk of bias assessment showed one high quality study, while the remaining 10 had questionable quality.
Conclusions: The effects of storage media on in-vitro dental material testing are variable and substrate-dependent, with dentine showing greater susceptibility than enamel. While distilled water and cryopreservation showed relative stability, other media like thymol and Chloramine T produced inconsistent results. Standardized protocols and further research are needed to ensure reliable and comparable outcomes.
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| format | Article |
| id | doaj-art-d30c7dfcc9f7413aa4be86e3749fbcfc |
| institution | Matheson Library |
| issn | 2641-5275 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Medical Journals Sweden |
| record_format | Article |
| series | Biomaterial Investigations in Dentistry |
| spelling | doaj-art-d30c7dfcc9f7413aa4be86e3749fbcfc2025-07-22T07:33:41ZengMedical Journals SwedenBiomaterial Investigations in Dentistry2641-52752025-07-0112110.2340/biid.v12.44183Effect of storage medium of extracted human teeth on the result of in vitro studies – a scoping reviewMuhammad Sadiq Billoo0Muhammad Ahsan Kaleem Khan Khan1Taimur Khalid2Syed Murtaza Raza Kazmi3Department of Dentistry and Oral Health Sciences, The Aga Khan University, Karachi, PakistanDepartment of Dentistry and Oral Health Sciences, The Aga Khan University, Karachi, PakistanDepartment of Dentistry and Oral Health Sciences, The Aga Khan University, Karachi, PakistanDepartment of Dentistry and Oral Health Sciences, The Aga Khan University, Karachi, Pakistan Purpose: This scoping review aimed to investigate how different storage media of extracted human teeth before and after preparation of specimens influence the outcomes of various dental materials intended for clinical application. Materials and methods: Literature search in four databases and by manual searching was carried out on a predefined PIO as follows: Population – extracted human teeth; Intervention – different storage media; Outcome(s) – material properties. Results: After screening 110 studies, 11 were included in the scoping review. Eight studies examined storage media effects before specimen preparation and three after specimen preparation. Four studies focused on enamel bonding, six on dentine, and one both on enamel and dentine. Concerning storage of extracted teeth prior to specimen preparation, cryopreservation, Chloramine T and thymol had no negative effect on bond strength to enamel. One study found Chloramine T to increase microleakage at enamel margins whereas another study found no negative effect. Storage in formalin, ethanol and thymol also had no negative effect on microleakage at enamel margins. Cryopreservation decreased bond strength to coronal dentine, but not to mid-coronal or deep dentine. Formalin, Chloramine T, ethanol and thymol had no negative effect on bond strength to dentine nor on microleakage at dentine margins. However, one study found long-term storage in Chloramine T to increase microleakage at dentine margins. Concerning storage of extracted teeth after specimen preparation, thymol significantly lowered bond strength to enamel, whereas formalin had no negative effect. Sodium hypochlorite had no negative effect on bond strength to dentine, and finally, formalin, Chloramine T, ethanol, and thymol had no negative effect on microleakage at dentine margins. Risk of bias assessment showed one high quality study, while the remaining 10 had questionable quality. Conclusions: The effects of storage media on in-vitro dental material testing are variable and substrate-dependent, with dentine showing greater susceptibility than enamel. While distilled water and cryopreservation showed relative stability, other media like thymol and Chloramine T produced inconsistent results. Standardized protocols and further research are needed to ensure reliable and comparable outcomes. https://medicaljournalssweden.se/biid/article/view/44183Storage mediacompositeresinsmicroleakagebond strengthmicromechanical properties |
| spellingShingle | Muhammad Sadiq Billoo Muhammad Ahsan Kaleem Khan Khan Taimur Khalid Syed Murtaza Raza Kazmi Effect of storage medium of extracted human teeth on the result of in vitro studies – a scoping review Biomaterial Investigations in Dentistry Storage media composite resins microleakage bond strength micromechanical properties |
| title | Effect of storage medium of extracted human teeth on the result of in vitro studies – a scoping review |
| title_full | Effect of storage medium of extracted human teeth on the result of in vitro studies – a scoping review |
| title_fullStr | Effect of storage medium of extracted human teeth on the result of in vitro studies – a scoping review |
| title_full_unstemmed | Effect of storage medium of extracted human teeth on the result of in vitro studies – a scoping review |
| title_short | Effect of storage medium of extracted human teeth on the result of in vitro studies – a scoping review |
| title_sort | effect of storage medium of extracted human teeth on the result of in vitro studies a scoping review |
| topic | Storage media composite resins microleakage bond strength micromechanical properties |
| url | https://medicaljournalssweden.se/biid/article/view/44183 |
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