ALTERNATIVE METHOD FOR DETERMINING METAL UPTAKE FROM WATER BY ESCHERICHIA COLI K12 CD3
Bacteria are usually applied in biotechnology in areas such as element adsorptions due to extracellular/intracellular accumulation. Electron microscopes and spectrophotometry have shown proximate/accurate methods for observing and detecting these accumulated elements. In contrast, X-ray fluorescence...
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University of Anbar
2025-06-01
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| Series: | مجلة الأنبار للعلوم الزراعية |
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| Online Access: | https://ajas.uoanbar.edu.iq/article_187524_48061a32ab39aa36662f02c2b1d15956.pdf |
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| author | N. M. T. Jebril A. K. Murad R. A. Majeed A. H. Al-Sabary I. A. Abed B. H. Hashim |
| author_facet | N. M. T. Jebril A. K. Murad R. A. Majeed A. H. Al-Sabary I. A. Abed B. H. Hashim |
| author_sort | N. M. T. Jebril |
| collection | DOAJ |
| description | Bacteria are usually applied in biotechnology in areas such as element adsorptions due to extracellular/intracellular accumulation. Electron microscopes and spectrophotometry have shown proximate/accurate methods for observing and detecting these accumulated elements. In contrast, X-ray fluorescence (XRF) validates the examination of elements, and direct investigation for studying these collected elements. This research assessed the prospects for applying XRF in examining samples of bacterial-accumulated cadmium, Escherichia coli K12 Cd3, from artificial wastewater (AWW) by comparing the commonly used techniques of transmission electron microscopy (TEM-EDX) and inductively-coupled plasma mass spectrometry (ICP-MS). The results document a new means for using the XRF device in determining accurate quantities of cadmium accumulated in bacterial cells. By comparison, usual TEM-EDX analyzes show that the concentration was not qualitatively determined. At the same time, the ICP-M device was not as efficient in measuring cadmium concentrations. Finally, the findings show that an XRF device directly measures the element within bacterial cells with greater accuracy than those analyzed by TEM-EDX and ICP-MS devices. This demonstrates that XRF can be applied in various areas of biotechnology to investigate the absorption of accumulated elements and to explain the procedures underlying the biotechnology process |
| format | Article |
| id | doaj-art-d2d6c8b77e5e48ea85e44ad1ff426f48 |
| institution | Matheson Library |
| issn | 1992-7479 2617-6211 |
| language | Arabic |
| publishDate | 2025-06-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة الأنبار للعلوم الزراعية |
| spelling | doaj-art-d2d6c8b77e5e48ea85e44ad1ff426f482025-07-08T21:33:11ZaraUniversity of Anbarمجلة الأنبار للعلوم الزراعية1992-74792617-62112025-06-0123152853910.32649/ajas.2025.187524187524ALTERNATIVE METHOD FOR DETERMINING METAL UPTAKE FROM WATER BY ESCHERICHIA COLI K12 CD3N. M. T. Jebril0A. K. Murad1R. A. Majeed2A. H. Al-Sabary3I. A. Abed4B. H. Hashim5Dept. of Biology, College of Sciences for Women, University of Babylon, IraqDept. of Sciences, College of Basic Education, University of Babylon, IraqCollege of Dentistry, University of Al-Ameed, Iraq – Karbala, IraqDept. of Biology, College of Sciences, Al-Mustaqbal University, 51001, Babylon, IraqSoil and Water resources, College of Agriculture, University of Anbar, IraqDept. of Pharmacology and Toxicology, College of Pharmacy, University of Babylon, IraqBacteria are usually applied in biotechnology in areas such as element adsorptions due to extracellular/intracellular accumulation. Electron microscopes and spectrophotometry have shown proximate/accurate methods for observing and detecting these accumulated elements. In contrast, X-ray fluorescence (XRF) validates the examination of elements, and direct investigation for studying these collected elements. This research assessed the prospects for applying XRF in examining samples of bacterial-accumulated cadmium, Escherichia coli K12 Cd3, from artificial wastewater (AWW) by comparing the commonly used techniques of transmission electron microscopy (TEM-EDX) and inductively-coupled plasma mass spectrometry (ICP-MS). The results document a new means for using the XRF device in determining accurate quantities of cadmium accumulated in bacterial cells. By comparison, usual TEM-EDX analyzes show that the concentration was not qualitatively determined. At the same time, the ICP-M device was not as efficient in measuring cadmium concentrations. Finally, the findings show that an XRF device directly measures the element within bacterial cells with greater accuracy than those analyzed by TEM-EDX and ICP-MS devices. This demonstrates that XRF can be applied in various areas of biotechnology to investigate the absorption of accumulated elements and to explain the procedures underlying the biotechnology processhttps://ajas.uoanbar.edu.iq/article_187524_48061a32ab39aa36662f02c2b1d15956.pdfaccumulation of elementsbacteriabioremediationcadmiumicp-mstemxrf |
| spellingShingle | N. M. T. Jebril A. K. Murad R. A. Majeed A. H. Al-Sabary I. A. Abed B. H. Hashim ALTERNATIVE METHOD FOR DETERMINING METAL UPTAKE FROM WATER BY ESCHERICHIA COLI K12 CD3 مجلة الأنبار للعلوم الزراعية accumulation of elements bacteria bioremediation cadmium icp-ms tem xrf |
| title | ALTERNATIVE METHOD FOR DETERMINING METAL UPTAKE FROM WATER BY ESCHERICHIA COLI K12 CD3 |
| title_full | ALTERNATIVE METHOD FOR DETERMINING METAL UPTAKE FROM WATER BY ESCHERICHIA COLI K12 CD3 |
| title_fullStr | ALTERNATIVE METHOD FOR DETERMINING METAL UPTAKE FROM WATER BY ESCHERICHIA COLI K12 CD3 |
| title_full_unstemmed | ALTERNATIVE METHOD FOR DETERMINING METAL UPTAKE FROM WATER BY ESCHERICHIA COLI K12 CD3 |
| title_short | ALTERNATIVE METHOD FOR DETERMINING METAL UPTAKE FROM WATER BY ESCHERICHIA COLI K12 CD3 |
| title_sort | alternative method for determining metal uptake from water by escherichia coli k12 cd3 |
| topic | accumulation of elements bacteria bioremediation cadmium icp-ms tem xrf |
| url | https://ajas.uoanbar.edu.iq/article_187524_48061a32ab39aa36662f02c2b1d15956.pdf |
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