Immunoglobulin Y antibodies against colonization-related genes block the growth and infection of Helicobacter pylori
IntroductionImmunoglobulin Y (IgY) has emerged as a promising antibody therapy for the eradication of Helicobacter pylori (H. pylori) independent of antibiotics. However, the roles and differences of IgY antibodies targeting various genes against H. pylori remain unclear.MethodsThe recombinant antig...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1582250/full |
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| author | Shiyuan Deng Xiaoling Luo Yunxiao Du Rania G. Elbaiomy Weihan He Rong Guo Ahmed H. El-Sappah Xiaohong Jian Yongmei Xie Mohammed Bakeer Mohammed Bakeer Zaixin Li Zhi Zhang |
| author_facet | Shiyuan Deng Xiaoling Luo Yunxiao Du Rania G. Elbaiomy Weihan He Rong Guo Ahmed H. El-Sappah Xiaohong Jian Yongmei Xie Mohammed Bakeer Mohammed Bakeer Zaixin Li Zhi Zhang |
| author_sort | Shiyuan Deng |
| collection | DOAJ |
| description | IntroductionImmunoglobulin Y (IgY) has emerged as a promising antibody therapy for the eradication of Helicobacter pylori (H. pylori) independent of antibiotics. However, the roles and differences of IgY antibodies targeting various genes against H. pylori remain unclear.MethodsThe recombinant antigens of five colonization-related genes — FlaA, BabA2, NapA, HpaA, and UreB — are prepared using a prokaryotic expression system and then subject to immunize laying hens for IgY production. Subsequently, their biological activities are evaluated, including blocking bacterial growth, attenuating infection in GES-1 cells, and eradicating H. pylori in gastritis mouse models.ResultsThese IgY antibodies can recognize the full-length antigens of H. pylori and exhibit a direct inhibitory effect on the growth and infection of H. pylori with dose-dependent characteristics. Among these, anti-FlaA IgY shows greater antibacterial activity in inhibiting H. pylori growth and preventing adhesion to GES-1 cells. Oral administration of these IgY antibodies for two weeks (20.0 mg·kg−1·day−1) achieves a 25% to 37.5% eradication rate of H. pylori infection in mice. Interestingly, combination treatment with these IgY antibodies, based on their different roles, enhances antibacterial benefits and significantly promotes the recovery of gastrointestinal function.ConclusionOur results indicate that IgY antibodies against colonization-related genes can directly block the growth and infection of H. pylori, and combination treatment with these antibodies offers more advantages in combating H. pylori. |
| format | Article |
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| institution | Matheson Library |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-d2430a629bbe4546bfa5aab5cb72f9ba2025-07-03T14:38:01ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-06-011610.3389/fimmu.2025.15822501582250Immunoglobulin Y antibodies against colonization-related genes block the growth and infection of Helicobacter pyloriShiyuan Deng0Xiaoling Luo1Yunxiao Du2Rania G. Elbaiomy3Weihan He4Rong Guo5Ahmed H. El-Sappah6Xiaohong Jian7Yongmei Xie8Mohammed Bakeer9Mohammed Bakeer10Zaixin Li11Zhi Zhang12Department of Biological Engineering, Sichuan University of Science & Engineering, Zigong, ChinaDepartment of Gastroenterology, Fushun People’s Hospital, Zigong, ChinaDepartment of Biological Engineering, Sichuan University of Science & Engineering, Zigong, ChinaDepartment of Biological Engineering, Sichuan University of Science & Engineering, Zigong, ChinaDepartment of Biological Engineering, Sichuan University of Science & Engineering, Zigong, ChinaDepartment of Gastroenterology, Fushun People’s Hospital, Zigong, ChinaDepartment of Genetics, Faculty of Agriculture, Zagazig University, Zagazig, EgyptDepartment of Biological Engineering, Sichuan University of Science & Engineering, Zigong, ChinaState Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, ChinaDivision of Hematology and Medical Oncology, Mayo Clinic, Jacksonville, FL, United StatesDivision of Internal Medicine-Clinical Hematology, Al-Azhar University, Cairo, EgyptDepartment of Biological Engineering, Sichuan University of Science & Engineering, Zigong, ChinaDepartment of Biological Engineering, Sichuan University of Science & Engineering, Zigong, ChinaIntroductionImmunoglobulin Y (IgY) has emerged as a promising antibody therapy for the eradication of Helicobacter pylori (H. pylori) independent of antibiotics. However, the roles and differences of IgY antibodies targeting various genes against H. pylori remain unclear.MethodsThe recombinant antigens of five colonization-related genes — FlaA, BabA2, NapA, HpaA, and UreB — are prepared using a prokaryotic expression system and then subject to immunize laying hens for IgY production. Subsequently, their biological activities are evaluated, including blocking bacterial growth, attenuating infection in GES-1 cells, and eradicating H. pylori in gastritis mouse models.ResultsThese IgY antibodies can recognize the full-length antigens of H. pylori and exhibit a direct inhibitory effect on the growth and infection of H. pylori with dose-dependent characteristics. Among these, anti-FlaA IgY shows greater antibacterial activity in inhibiting H. pylori growth and preventing adhesion to GES-1 cells. Oral administration of these IgY antibodies for two weeks (20.0 mg·kg−1·day−1) achieves a 25% to 37.5% eradication rate of H. pylori infection in mice. Interestingly, combination treatment with these IgY antibodies, based on their different roles, enhances antibacterial benefits and significantly promotes the recovery of gastrointestinal function.ConclusionOur results indicate that IgY antibodies against colonization-related genes can directly block the growth and infection of H. pylori, and combination treatment with these antibodies offers more advantages in combating H. pylori.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1582250/fullimmunoglobulin YHelicobacter pylorigrowthinfectioneradication rate |
| spellingShingle | Shiyuan Deng Xiaoling Luo Yunxiao Du Rania G. Elbaiomy Weihan He Rong Guo Ahmed H. El-Sappah Xiaohong Jian Yongmei Xie Mohammed Bakeer Mohammed Bakeer Zaixin Li Zhi Zhang Immunoglobulin Y antibodies against colonization-related genes block the growth and infection of Helicobacter pylori Frontiers in Immunology immunoglobulin Y Helicobacter pylori growth infection eradication rate |
| title | Immunoglobulin Y antibodies against colonization-related genes block the growth and infection of Helicobacter pylori |
| title_full | Immunoglobulin Y antibodies against colonization-related genes block the growth and infection of Helicobacter pylori |
| title_fullStr | Immunoglobulin Y antibodies against colonization-related genes block the growth and infection of Helicobacter pylori |
| title_full_unstemmed | Immunoglobulin Y antibodies against colonization-related genes block the growth and infection of Helicobacter pylori |
| title_short | Immunoglobulin Y antibodies against colonization-related genes block the growth and infection of Helicobacter pylori |
| title_sort | immunoglobulin y antibodies against colonization related genes block the growth and infection of helicobacter pylori |
| topic | immunoglobulin Y Helicobacter pylori growth infection eradication rate |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1582250/full |
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