AI-Powered Spectral Imaging for Virtual Pathology Staining
Pathological analysis of tissue biopsies remains the gold standard for diagnosing cancer and other diseases. However, this is a time-intensive process that demands extensive training and expertise. Despite its importance, it is often subjective and not entirely error-free. Over the past decade, path...
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MDPI AG
2025-06-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/12/6/655 |
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| author | Adam Soker Maya Almagor Sabine Mai Yuval Garini |
| author_facet | Adam Soker Maya Almagor Sabine Mai Yuval Garini |
| author_sort | Adam Soker |
| collection | DOAJ |
| description | Pathological analysis of tissue biopsies remains the gold standard for diagnosing cancer and other diseases. However, this is a time-intensive process that demands extensive training and expertise. Despite its importance, it is often subjective and not entirely error-free. Over the past decade, pathology has undergone two major transformations. First, the rise in whole slide imaging has enabled work in front of a computer screen and the integration of image processing tools to enhance diagnostics. Second, the rapid evolution of Artificial Intelligence has revolutionized numerous fields and has had a remarkable impact on humanity. The synergy of these two has paved the way for groundbreaking research aiming for advancements in digital pathology. Despite encouraging research outcomes, AI-based tools have yet to be actively incorporated into therapeutic protocols. This is primary due to the need for high reliability in medical therapy, necessitating a new approach that ensures greater robustness. Another approach for improving pathological diagnosis involves advanced optical methods such as spectral imaging, which reveals information from the tissue that is beyond human vision. We have recently developed a unique rapid spectral imaging system capable of scanning pathological slides, delivering a wealth of critical diagnostic information. Here, we present a novel application of spectral imaging (SI) for virtual Hematoxylin and Eosin (H&E) staining using a custom-built, rapid Fourier-based SI system. Unstained human biopsy samples are scanned, and a Pix2Pix-based neural network generates realistic H&E-equivalent images. Additionally, we applied Principal Component Analysis (PCA) to the spectral information to examine the effect of down sampling the data on the virtual staining process. To assess model performance, we trained and tested models using full spectral data, RGB, and PCA-reduced spectral inputs. The results demonstrate that PCA-reduced data preserved essential image features while enhancing statistical image quality, as indicated by FID and KID scores, and reducing computational complexity. These findings highlight the potential of integrating SI and AI to enable efficient, accurate, and stain-free digital pathology. |
| format | Article |
| id | doaj-art-d1e59280e9e64ee7aaa9b51cc4282d67 |
| institution | Matheson Library |
| issn | 2306-5354 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj-art-d1e59280e9e64ee7aaa9b51cc4282d672025-06-25T13:30:03ZengMDPI AGBioengineering2306-53542025-06-0112665510.3390/bioengineering12060655AI-Powered Spectral Imaging for Virtual Pathology StainingAdam Soker0Maya Almagor1Sabine Mai2Yuval Garini3Biomedical Engineering Faculty & Russell Berrie Nanotechnology Institute, Technion—Israel Institute of Technology, Haifa 3200003, IsraelBiomedical Engineering Faculty & Russell Berrie Nanotechnology Institute, Technion—Israel Institute of Technology, Haifa 3200003, IsraelDepartment of Physiology and Pathophysiology, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaBiomedical Engineering Faculty & Russell Berrie Nanotechnology Institute, Technion—Israel Institute of Technology, Haifa 3200003, IsraelPathological analysis of tissue biopsies remains the gold standard for diagnosing cancer and other diseases. However, this is a time-intensive process that demands extensive training and expertise. Despite its importance, it is often subjective and not entirely error-free. Over the past decade, pathology has undergone two major transformations. First, the rise in whole slide imaging has enabled work in front of a computer screen and the integration of image processing tools to enhance diagnostics. Second, the rapid evolution of Artificial Intelligence has revolutionized numerous fields and has had a remarkable impact on humanity. The synergy of these two has paved the way for groundbreaking research aiming for advancements in digital pathology. Despite encouraging research outcomes, AI-based tools have yet to be actively incorporated into therapeutic protocols. This is primary due to the need for high reliability in medical therapy, necessitating a new approach that ensures greater robustness. Another approach for improving pathological diagnosis involves advanced optical methods such as spectral imaging, which reveals information from the tissue that is beyond human vision. We have recently developed a unique rapid spectral imaging system capable of scanning pathological slides, delivering a wealth of critical diagnostic information. Here, we present a novel application of spectral imaging (SI) for virtual Hematoxylin and Eosin (H&E) staining using a custom-built, rapid Fourier-based SI system. Unstained human biopsy samples are scanned, and a Pix2Pix-based neural network generates realistic H&E-equivalent images. Additionally, we applied Principal Component Analysis (PCA) to the spectral information to examine the effect of down sampling the data on the virtual staining process. To assess model performance, we trained and tested models using full spectral data, RGB, and PCA-reduced spectral inputs. The results demonstrate that PCA-reduced data preserved essential image features while enhancing statistical image quality, as indicated by FID and KID scores, and reducing computational complexity. These findings highlight the potential of integrating SI and AI to enable efficient, accurate, and stain-free digital pathology.https://www.mdpi.com/2306-5354/12/6/655spectral imagingvirtual stainingdigital pathologyartificial intelligence in medicine |
| spellingShingle | Adam Soker Maya Almagor Sabine Mai Yuval Garini AI-Powered Spectral Imaging for Virtual Pathology Staining Bioengineering spectral imaging virtual staining digital pathology artificial intelligence in medicine |
| title | AI-Powered Spectral Imaging for Virtual Pathology Staining |
| title_full | AI-Powered Spectral Imaging for Virtual Pathology Staining |
| title_fullStr | AI-Powered Spectral Imaging for Virtual Pathology Staining |
| title_full_unstemmed | AI-Powered Spectral Imaging for Virtual Pathology Staining |
| title_short | AI-Powered Spectral Imaging for Virtual Pathology Staining |
| title_sort | ai powered spectral imaging for virtual pathology staining |
| topic | spectral imaging virtual staining digital pathology artificial intelligence in medicine |
| url | https://www.mdpi.com/2306-5354/12/6/655 |
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