Soybean <i>GmSNF4</i> Confers Salt–Alkali Stress Tolerance in Transgenic Plants
In order to mitigate the reduction in soybean yield caused by soil salinization, a soybean gene, <i>GmSNF4</i>, which promotes plant tolerance to salt–alkali stress, was identified in this study. The STRING database was used to predict the interaction between GmSNF4 and GmPKS4. The <i...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/14/2218 |
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| Summary: | In order to mitigate the reduction in soybean yield caused by soil salinization, a soybean gene, <i>GmSNF4</i>, which promotes plant tolerance to salt–alkali stress, was identified in this study. The STRING database was used to predict the interaction between GmSNF4 and GmPKS4. The <i>GmPKS4</i> gene was experimentally shown to be involved in salt–alkali stress tolerance. Firstly, the yeast two-hybrid technique and bimolecular fluorescence complementation (BiFC) technique were used to confirm the interaction between GmSNF4 and GmPKS4: the AMPK-CBM-CBS1 conserved domain was thereby determined to be the region of the GmSNF4 protein involved in the interaction. Secondly, the <i>GmSNF4</i> gene was induced by salt–alkali stress according to qRT-PCR analysis, and the GmSNF4 protein was localized in the nucleus and cytoplasm. Finally, analysis of <i>GmSNF4</i>’s role in resistance to salt–alkali stress in transgenic soybean plants showed that transgenic lines had better phenotypic, physiological, and stress-related gene expression than non-transgenic soybeans. Thus, <i>GmSNF4</i> may play a significant role in plant salt–alkali stress tolerance. |
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| ISSN: | 2223-7747 |