Exercise, Training & Health
Rationale: Anti-SARS-CoV-2 antibody responses elicited by infection or vaccination vary among individuals and over time. Their knowledge is of particular importance in, amongst others, elite sport. Can dried blood spots serve as a minimally-invasive, low-cost, decentralized tool to monitor the quant...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | German |
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SportMed Verlag
2022-03-01
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| Series: | Deutsche Zeitschrift für Sportmedizin |
| Online Access: | https://www.germanjournalsportsmedicine.com/archive/archive-2022/issue-2/dried-blood-spots-to-monitor-the-anti-sars-cov-2-antibody-response-after-infection-or-vaccination/ |
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| _version_ | 1839597045875212288 |
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| author | Carthaus A Görgens C Knoop A Geyer H Thevis M |
| author_facet | Carthaus A Görgens C Knoop A Geyer H Thevis M |
| author_sort | Carthaus A |
| collection | DOAJ |
| description | Rationale: Anti-SARS-CoV-2 antibody responses elicited by infection or vaccination vary among individuals and over time. Their knowledge is of particular importance in, amongst others, elite sport. Can dried blood spots serve as a minimally-invasive, low-cost, decentralized tool to monitor the quantitative antibody response and thus represent an alternative to full blood tests?Methods: Cross-validation of dried blood and venous blood samples of 27 individuals post-infection and 96 individuals post-vaccination, longitudinal antibody monitoring of 27 individuals after vaccination using different vaccines and vaccine schedules and detection of seropositive individuals in a cohort of 557 people using self-collected DBS (dried blood spots) and two commercial immunoassays.Findings: Plasma and DBS values were highly correlated allowing for extrapolation of plasma values from DBS using a factor of a least 10 following the presented procedure. Capillary volumetric sampling and self-sampling produced reliable results. After vaccination, participants showed heterogenous antibody responses but a consistent increase after the second dose. DBS allowed for the analysis of a huge sample volume in a timely manner by limited laboratory personnel.Discussion: DBS offer the possibility of infection and vaccination traceability of individuals and cohorts via minimally-invasive self-sampling. This way, they allow to screen and monitor the presence and evolution of anti-SARS-CoV-2 antibodies in a qualitative and quantitative manner. Using two commercial, automated assays enables large-scale and frequent testing, global implementation and comparability of results.Key Words: Coronavirus, Pandemic, Diagnostics, Immunity, DBS |
| format | Article |
| id | doaj-art-cfa0b6852e77435e84dfdaac82efeb9d |
| institution | Matheson Library |
| issn | 0344-5925 2510-5264 |
| language | deu |
| publishDate | 2022-03-01 |
| publisher | SportMed Verlag |
| record_format | Article |
| series | Deutsche Zeitschrift für Sportmedizin |
| spelling | doaj-art-cfa0b6852e77435e84dfdaac82efeb9d2025-08-02T19:54:09ZdeuSportMed VerlagDeutsche Zeitschrift für Sportmedizin0344-59252510-52642022-03-0173210.5960/dzsm.2021.519309912Exercise, Training & HealthCarthaus AGörgens CKnoop AGeyer HThevis MRationale: Anti-SARS-CoV-2 antibody responses elicited by infection or vaccination vary among individuals and over time. Their knowledge is of particular importance in, amongst others, elite sport. Can dried blood spots serve as a minimally-invasive, low-cost, decentralized tool to monitor the quantitative antibody response and thus represent an alternative to full blood tests?Methods: Cross-validation of dried blood and venous blood samples of 27 individuals post-infection and 96 individuals post-vaccination, longitudinal antibody monitoring of 27 individuals after vaccination using different vaccines and vaccine schedules and detection of seropositive individuals in a cohort of 557 people using self-collected DBS (dried blood spots) and two commercial immunoassays.Findings: Plasma and DBS values were highly correlated allowing for extrapolation of plasma values from DBS using a factor of a least 10 following the presented procedure. Capillary volumetric sampling and self-sampling produced reliable results. After vaccination, participants showed heterogenous antibody responses but a consistent increase after the second dose. DBS allowed for the analysis of a huge sample volume in a timely manner by limited laboratory personnel.Discussion: DBS offer the possibility of infection and vaccination traceability of individuals and cohorts via minimally-invasive self-sampling. This way, they allow to screen and monitor the presence and evolution of anti-SARS-CoV-2 antibodies in a qualitative and quantitative manner. Using two commercial, automated assays enables large-scale and frequent testing, global implementation and comparability of results.Key Words: Coronavirus, Pandemic, Diagnostics, Immunity, DBShttps://www.germanjournalsportsmedicine.com/archive/archive-2022/issue-2/dried-blood-spots-to-monitor-the-anti-sars-cov-2-antibody-response-after-infection-or-vaccination/ |
| spellingShingle | Carthaus A Görgens C Knoop A Geyer H Thevis M Exercise, Training & Health Deutsche Zeitschrift für Sportmedizin |
| title | Exercise, Training & Health |
| title_full | Exercise, Training & Health |
| title_fullStr | Exercise, Training & Health |
| title_full_unstemmed | Exercise, Training & Health |
| title_short | Exercise, Training & Health |
| title_sort | exercise training amp health |
| url | https://www.germanjournalsportsmedicine.com/archive/archive-2022/issue-2/dried-blood-spots-to-monitor-the-anti-sars-cov-2-antibody-response-after-infection-or-vaccination/ |
| work_keys_str_mv | AT carthausa exercisetrainingamphealth AT gorgensc exercisetrainingamphealth AT knoopa exercisetrainingamphealth AT geyerh exercisetrainingamphealth AT thevism exercisetrainingamphealth |