Exercise, Training & Health
Rationale: Anti-SARS-CoV-2 antibody responses elicited by infection or vaccination vary among individuals and over time. Their knowledge is of particular importance in, amongst others, elite sport. Can dried blood spots serve as a minimally-invasive, low-cost, decentralized tool to monitor the quant...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | German |
| Published: |
SportMed Verlag
2022-03-01
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| Series: | Deutsche Zeitschrift für Sportmedizin |
| Online Access: | https://www.germanjournalsportsmedicine.com/archive/archive-2022/issue-2/dried-blood-spots-to-monitor-the-anti-sars-cov-2-antibody-response-after-infection-or-vaccination/ |
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| Summary: | Rationale: Anti-SARS-CoV-2 antibody responses elicited by infection or vaccination vary among individuals and over time. Their knowledge is of particular importance in, amongst others, elite sport. Can dried blood spots serve as a minimally-invasive, low-cost, decentralized tool to monitor the quantitative antibody response and thus represent an alternative to full blood tests?Methods: Cross-validation of dried blood and venous blood samples of 27 individuals post-infection and 96 individuals post-vaccination, longitudinal antibody monitoring of 27 individuals after vaccination using different vaccines and vaccine schedules and detection of seropositive individuals in a cohort of 557 people using self-collected DBS (dried blood spots) and two commercial immunoassays.Findings: Plasma and DBS values were highly correlated allowing for extrapolation of plasma values from DBS using a factor of a least 10 following the presented procedure. Capillary volumetric sampling and self-sampling produced reliable results. After vaccination, participants showed heterogenous antibody responses but a consistent increase after the second dose. DBS allowed for the analysis of a huge sample volume in a timely manner by limited laboratory personnel.Discussion: DBS offer the possibility of infection and vaccination traceability of individuals and cohorts via minimally-invasive self-sampling. This way, they allow to screen and monitor the presence and evolution of anti-SARS-CoV-2 antibodies in a qualitative and quantitative manner. Using two commercial, automated assays enables large-scale and frequent testing, global implementation and comparability of results.Key Words: Coronavirus, Pandemic, Diagnostics, Immunity, DBS |
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| ISSN: | 0344-5925 2510-5264 |