Isolation and Characterization of Laccase from <i>Trichoderma asperellum</i> Tasjk65
Laccase catalyzes one-electron oxidation, producing water as the primary final product, thereby minimizing secondary environmental pollution. Consequently, it holds significant application potential in areas such as the degradation of toxic compounds. In this study, a high-laccase-producing <i>...
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2025-06-01
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| author | Kehe Fu Lili Fan Qi Li Jiaming Ji Zhenying Huang Ting Huang |
| author_facet | Kehe Fu Lili Fan Qi Li Jiaming Ji Zhenying Huang Ting Huang |
| author_sort | Kehe Fu |
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| description | Laccase catalyzes one-electron oxidation, producing water as the primary final product, thereby minimizing secondary environmental pollution. Consequently, it holds significant application potential in areas such as the degradation of toxic compounds. In this study, a high-laccase-producing <i>Trichoderma</i> strain was isolated from soil, and the conditions for laccase production were optimized. Additionally, the laccase-related gene was cloned, and its function was analyzed. The results revealed that the optimal conditions for laccase production in this strain were maltose as the carbon source, peptone as the nitrogen source, an optimal pH of 6.0, and an incubation time of 120 h, resulting in an enzyme activity of 1.32 U/mL. The purified enzyme exhibited a Michaelis constant (<i>K<sub>m</sub></i>) of 0.06666 mmol/L when ABTS was used as the substrate. SDS-PAGE analysis indicated that the enzyme’s molecular weight was approximately 70 kDa. Sequencing of the target protein band led to the identification of the laccase-related gene <i>Tasla01</i>. Knockout of this gene resulted in the loss of laccase activity. We isolated a high-laccase-producing <i>Trichoderma asperellum</i> strain, Tasjk65, and cloned the laccase-related functional gene <i>Tasla01</i>. These findings lay a foundation for the source and application of laccase. |
| format | Article |
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| spelling | doaj-art-cf703d19d98b47f097b60283543f22782025-06-25T13:30:37ZengMDPI AGBiology2079-77372025-06-0114669110.3390/biology14060691Isolation and Characterization of Laccase from <i>Trichoderma asperellum</i> Tasjk65Kehe Fu0Lili Fan1Qi Li2Jiaming Ji3Zhenying Huang4Ting Huang5College of Life Science, Nanchang Normal University, Nanchang 330032, ChinaCollege of Life Science, Nanchang Normal University, Nanchang 330032, ChinaCollege of Life Science, Nanchang Normal University, Nanchang 330032, ChinaCollege of Life Science, Nanchang Normal University, Nanchang 330032, ChinaCollege of Life Science, Nanchang Normal University, Nanchang 330032, ChinaCollege of Life Science, Nanchang Normal University, Nanchang 330032, ChinaLaccase catalyzes one-electron oxidation, producing water as the primary final product, thereby minimizing secondary environmental pollution. Consequently, it holds significant application potential in areas such as the degradation of toxic compounds. In this study, a high-laccase-producing <i>Trichoderma</i> strain was isolated from soil, and the conditions for laccase production were optimized. Additionally, the laccase-related gene was cloned, and its function was analyzed. The results revealed that the optimal conditions for laccase production in this strain were maltose as the carbon source, peptone as the nitrogen source, an optimal pH of 6.0, and an incubation time of 120 h, resulting in an enzyme activity of 1.32 U/mL. The purified enzyme exhibited a Michaelis constant (<i>K<sub>m</sub></i>) of 0.06666 mmol/L when ABTS was used as the substrate. SDS-PAGE analysis indicated that the enzyme’s molecular weight was approximately 70 kDa. Sequencing of the target protein band led to the identification of the laccase-related gene <i>Tasla01</i>. Knockout of this gene resulted in the loss of laccase activity. We isolated a high-laccase-producing <i>Trichoderma asperellum</i> strain, Tasjk65, and cloned the laccase-related functional gene <i>Tasla01</i>. These findings lay a foundation for the source and application of laccase.https://www.mdpi.com/2079-7737/14/6/691<i>Trichoderma asperellum</i>laccaseenzyme kineticsgene knockout |
| spellingShingle | Kehe Fu Lili Fan Qi Li Jiaming Ji Zhenying Huang Ting Huang Isolation and Characterization of Laccase from <i>Trichoderma asperellum</i> Tasjk65 Biology <i>Trichoderma asperellum</i> laccase enzyme kinetics gene knockout |
| title | Isolation and Characterization of Laccase from <i>Trichoderma asperellum</i> Tasjk65 |
| title_full | Isolation and Characterization of Laccase from <i>Trichoderma asperellum</i> Tasjk65 |
| title_fullStr | Isolation and Characterization of Laccase from <i>Trichoderma asperellum</i> Tasjk65 |
| title_full_unstemmed | Isolation and Characterization of Laccase from <i>Trichoderma asperellum</i> Tasjk65 |
| title_short | Isolation and Characterization of Laccase from <i>Trichoderma asperellum</i> Tasjk65 |
| title_sort | isolation and characterization of laccase from i trichoderma asperellum i tasjk65 |
| topic | <i>Trichoderma asperellum</i> laccase enzyme kinetics gene knockout |
| url | https://www.mdpi.com/2079-7737/14/6/691 |
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