Effect of high-protein and low-carbohydrate diet on lipid and intestinal short-chain fatty acid secretion in C57BL/6 mice
Objective To investigate and formulate the healthy high-protein and low-carbohydrate diet pattern suitable for the normal weight group. Methods Twenty-four SPF grade male C57BL/6 mice aged 6 to 8 weeks （weighing 15 to 20 g） were randomly divided into the control group （Ct group）， 5% high-protein low...
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| Language: | Chinese |
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Editorial Office of Journal of New Medicine
2025-06-01
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| Series: | Xin yixue |
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| Online Access: | https://www.xinyixue.cn/fileup/0253-9802/PDF/1751417437178-1521377351.pdf |
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| author | HE Yuxi, LIU Ruonan, GONG Kai, YIMANALI Hulalai, LI Li, MAIMAITI Maiira, ZHAO Xiaoguo |
| author_facet | HE Yuxi, LIU Ruonan, GONG Kai, YIMANALI Hulalai, LI Li, MAIMAITI Maiira, ZHAO Xiaoguo |
| author_sort | HE Yuxi, LIU Ruonan, GONG Kai, YIMANALI Hulalai, LI Li, MAIMAITI Maiira, ZHAO Xiaoguo |
| collection | DOAJ |
| description | Objective To investigate and formulate the healthy high-protein and low-carbohydrate diet pattern suitable for the normal weight group. Methods Twenty-four SPF grade male C57BL/6 mice aged 6 to 8 weeks （weighing 15 to 20 g） were randomly divided into the control group （Ct group）， 5% high-protein low-carbohydrate diet group （5%LCD group）， and 30% high-protein low-carbohydrate diet group （30%LCD group）， with 8 mice in each group. The mice were fed with a basic diet， 5% LCD， and 30% LCD for 4 weeks， respectively. The food intake， water intake， and body weight were observed， and the blood lipid indexes and the content of cecal short-chain fatty acids were measured. Results Compared with the Ct group， food intake in the 5%LCD and 30%LCD groups was increased （both <i>P <</i> 0.01）. Compared with the Ct group， the serum total cholesterol （TC） and low-density lipoprotein cholesterol （LDL-C） contents in the 5%LCD group were increased （both <i>P <</i> 0.05）， and the serum LDL-C and high-density lipoprotein cholesterol （HDL-C） levels in the 30%LCD group were increased （both <i>P <</i> 0.05）. Compared with the Ct group， the secretions of propionic acid， isobutyric acid， and isovaleric acid in the cecal contents of mice in the 5%LCD and 30%LCD groups were decreased （all <i>P <</i> 0.05）. Conclusion Based on a comprehensive assessment of body weight， lipid metabolism， and short-chain fatty acid secretion， 30% LCD is more suitable as an effective dietary recommendation due to its higher HDL-C levels. |
| format | Article |
| id | doaj-art-cf676d88ebde4848a2f8f2e706a7cb41 |
| institution | Matheson Library |
| issn | 0253-9802 |
| language | zho |
| publishDate | 2025-06-01 |
| publisher | Editorial Office of Journal of New Medicine |
| record_format | Article |
| series | Xin yixue |
| spelling | doaj-art-cf676d88ebde4848a2f8f2e706a7cb412025-07-02T08:06:47ZzhoEditorial Office of Journal of New MedicineXin yixue0253-98022025-06-0156656857410.12464/j.issn.0253-9802.2024-0448Effect of high-protein and low-carbohydrate diet on lipid and intestinal short-chain fatty acid secretion in C57BL/6 miceHE Yuxi, LIU Ruonan, GONG Kai, YIMANALI Hulalai, LI Li, MAIMAITI Maiira, ZHAO Xiaoguo01. School of Public Health， Xinjiang Medical University， Urumqi 830011， China;2. Department of Clinical Nutrition， the First Affiliated Hospital of Xinjiang Medical University， Urumqi 830054， ChinaObjective To investigate and formulate the healthy high-protein and low-carbohydrate diet pattern suitable for the normal weight group. Methods Twenty-four SPF grade male C57BL/6 mice aged 6 to 8 weeks （weighing 15 to 20 g） were randomly divided into the control group （Ct group）， 5% high-protein low-carbohydrate diet group （5%LCD group）， and 30% high-protein low-carbohydrate diet group （30%LCD group）， with 8 mice in each group. The mice were fed with a basic diet， 5% LCD， and 30% LCD for 4 weeks， respectively. The food intake， water intake， and body weight were observed， and the blood lipid indexes and the content of cecal short-chain fatty acids were measured. Results Compared with the Ct group， food intake in the 5%LCD and 30%LCD groups was increased （both <i>P <</i> 0.01）. Compared with the Ct group， the serum total cholesterol （TC） and low-density lipoprotein cholesterol （LDL-C） contents in the 5%LCD group were increased （both <i>P <</i> 0.05）， and the serum LDL-C and high-density lipoprotein cholesterol （HDL-C） levels in the 30%LCD group were increased （both <i>P <</i> 0.05）. Compared with the Ct group， the secretions of propionic acid， isobutyric acid， and isovaleric acid in the cecal contents of mice in the 5%LCD and 30%LCD groups were decreased （all <i>P <</i> 0.05）. Conclusion Based on a comprehensive assessment of body weight， lipid metabolism， and short-chain fatty acid secretion， 30% LCD is more suitable as an effective dietary recommendation due to its higher HDL-C levels.https://www.xinyixue.cn/fileup/0253-9802/PDF/1751417437178-1521377351.pdfhigh-protein low-carbohydrate|lipid|short-chain fatty acid |
| spellingShingle | HE Yuxi, LIU Ruonan, GONG Kai, YIMANALI Hulalai, LI Li, MAIMAITI Maiira, ZHAO Xiaoguo Effect of high-protein and low-carbohydrate diet on lipid and intestinal short-chain fatty acid secretion in C57BL/6 mice Xin yixue high-protein low-carbohydrate|lipid|short-chain fatty acid |
| title | Effect of high-protein and low-carbohydrate diet on lipid and intestinal short-chain fatty acid secretion in C57BL/6 mice |
| title_full | Effect of high-protein and low-carbohydrate diet on lipid and intestinal short-chain fatty acid secretion in C57BL/6 mice |
| title_fullStr | Effect of high-protein and low-carbohydrate diet on lipid and intestinal short-chain fatty acid secretion in C57BL/6 mice |
| title_full_unstemmed | Effect of high-protein and low-carbohydrate diet on lipid and intestinal short-chain fatty acid secretion in C57BL/6 mice |
| title_short | Effect of high-protein and low-carbohydrate diet on lipid and intestinal short-chain fatty acid secretion in C57BL/6 mice |
| title_sort | effect of high protein and low carbohydrate diet on lipid and intestinal short chain fatty acid secretion in c57bl 6 mice |
| topic | high-protein low-carbohydrate|lipid|short-chain fatty acid |
| url | https://www.xinyixue.cn/fileup/0253-9802/PDF/1751417437178-1521377351.pdf |
| work_keys_str_mv | AT heyuxiliuruonangongkaiyimanalihulalaililimaimaitimaiirazhaoxiaoguo effectofhighproteinandlowcarbohydratedietonlipidandintestinalshortchainfattyacidsecretioninc57bl6mice |