Optimization of inter simple sequence repeats (ISSR) analysis as applied to preliminary study of genetic variation in Abies beshanzuensis M. H. Wu
Factors which affect the ISSR analysis of Abies beshanzuensis, such as annealing temperature and concentrations of template DNA, Taq DNA polymerase, Mg<sup>2+</sup> and dNTP, etc. were examined. The optimal ISSR-PCR reaction system (25 μL) in our experiments was as the following: 1 × Taq...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2005-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2005.03.0277 |
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