Optimization of inter simple sequence repeats (ISSR) analysis as applied to preliminary study of genetic variation in Abies beshanzuensis M. H. Wu
Factors which affect the ISSR analysis of Abies beshanzuensis, such as annealing temperature and concentrations of template DNA, Taq DNA polymerase, Mg<sup>2+</sup> and dNTP, etc. were examined. The optimal ISSR-PCR reaction system (25 μL) in our experiments was as the following: 1 × Taq...
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Zhejiang University Press
2005-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2005.03.0277 |
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| author | AI Jian-guo QIU Ying-xiong YU Jiu-hua CHEN Xiao-rong DING Bing-yang |
| author_facet | AI Jian-guo QIU Ying-xiong YU Jiu-hua CHEN Xiao-rong DING Bing-yang |
| author_sort | AI Jian-guo |
| collection | DOAJ |
| description | Factors which affect the ISSR analysis of Abies beshanzuensis, such as annealing temperature and concentrations of template DNA, Taq DNA polymerase, Mg<sup>2+</sup> and dNTP, etc. were examined. The optimal ISSR-PCR reaction system (25 μL) in our experiments was as the following: 1 × Taq buffer, 40 ng template DNA, 1.2 U Taq DNA polymerase, 0.4 μmol/L primer, 2.0 mmol/L MgCl<sub>2</sub> 0.2 mmol/L dNTPs and 2% formamide. Of the 100 primers subjected to screening, 13 produced highly reproducible and polymorphic bands. Using these primers, 91 discernible DNA fragments were generated with 39(42.86%) being polymorphic bands, indicating the existence of low genetic variation in the species. The level of genetic variation in population originated from seedlings (PPB=37.36%) was higher than that in population of old trees (PPB=16.48%) and population of graft-originated (PPB=20.88%), which suggested that cross-pollination by human beings was helpful to increasing genetic diversity of A. beshanzuensis. |
| format | Article |
| id | doaj-art-cceb6fa6d3ae450c9f3f32fd9b2914e7 |
| institution | Matheson Library |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2005-05-01 |
| publisher | Zhejiang University Press |
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| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-cceb6fa6d3ae450c9f3f32fd9b2914e72025-08-01T05:38:14ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552005-05-013127728210.3785/1008-9209.2005.03.027710089209Optimization of inter simple sequence repeats (ISSR) analysis as applied to preliminary study of genetic variation in Abies beshanzuensis M. H. WuAI Jian-guoQIU Ying-xiongYU Jiu-huaCHEN Xiao-rongDING Bing-yangFactors which affect the ISSR analysis of Abies beshanzuensis, such as annealing temperature and concentrations of template DNA, Taq DNA polymerase, Mg<sup>2+</sup> and dNTP, etc. were examined. The optimal ISSR-PCR reaction system (25 μL) in our experiments was as the following: 1 × Taq buffer, 40 ng template DNA, 1.2 U Taq DNA polymerase, 0.4 μmol/L primer, 2.0 mmol/L MgCl<sub>2</sub> 0.2 mmol/L dNTPs and 2% formamide. Of the 100 primers subjected to screening, 13 produced highly reproducible and polymorphic bands. Using these primers, 91 discernible DNA fragments were generated with 39(42.86%) being polymorphic bands, indicating the existence of low genetic variation in the species. The level of genetic variation in population originated from seedlings (PPB=37.36%) was higher than that in population of old trees (PPB=16.48%) and population of graft-originated (PPB=20.88%), which suggested that cross-pollination by human beings was helpful to increasing genetic diversity of A. beshanzuensis.https://www.academax.com/doi/10.3785/1008-9209.2005.03.0277ISSRoptimizationgenetic variation<italic>Abies beshanzuensis</italic> |
| spellingShingle | AI Jian-guo QIU Ying-xiong YU Jiu-hua CHEN Xiao-rong DING Bing-yang Optimization of inter simple sequence repeats (ISSR) analysis as applied to preliminary study of genetic variation in Abies beshanzuensis M. H. Wu 浙江大学学报. 农业与生命科学版 ISSR optimization genetic variation <italic>Abies beshanzuensis</italic> |
| title | Optimization of inter simple sequence repeats (ISSR) analysis as applied to preliminary study of genetic variation in Abies beshanzuensis M. H. Wu |
| title_full | Optimization of inter simple sequence repeats (ISSR) analysis as applied to preliminary study of genetic variation in Abies beshanzuensis M. H. Wu |
| title_fullStr | Optimization of inter simple sequence repeats (ISSR) analysis as applied to preliminary study of genetic variation in Abies beshanzuensis M. H. Wu |
| title_full_unstemmed | Optimization of inter simple sequence repeats (ISSR) analysis as applied to preliminary study of genetic variation in Abies beshanzuensis M. H. Wu |
| title_short | Optimization of inter simple sequence repeats (ISSR) analysis as applied to preliminary study of genetic variation in Abies beshanzuensis M. H. Wu |
| title_sort | optimization of inter simple sequence repeats issr analysis as applied to preliminary study of genetic variation in abies beshanzuensis m h wu |
| topic | ISSR optimization genetic variation <italic>Abies beshanzuensis</italic> |
| url | https://www.academax.com/doi/10.3785/1008-9209.2005.03.0277 |
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