Novel drug-inducible CRISPRa/i systems for rapid and reversible manipulation of gene transcription

Novel drug-inducible CRISPRa/i systems for rapid and reversible manipulation of gene transcription

Abstract CRISPR activation and interference (CRISPRa/i) are highly effective tools to regulate transcription by fusing dead Cas9 (dCas9) with transcriptional regulatory factors guided by small guide RNA (sgRNA) in mammalian cells and mice. Still, a controllable gene regulation system is desired to i...

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Bibliographic Details
Main Authors: Ming Sui, Meiling Zhou, Mengge Cui, Huan Liu, Xiaolin Zhang, Na Hu, Yang Li, Beibei Wang, Guojun Yang, Pengling Gui, Lingqiang Zhu, Feng Wan, Bin Zhang
Format: Article
Language:English
Published: Springer 2025-06-01
Series:Cellular and Molecular Life Sciences
Subjects:
iCRISPRa/i
4OHT
Transcriptional regulation
Phenotypic change
Online Access:https://doi.org/10.1007/s00018-025-05786-7
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