Fe-deficiency-induced chlorosis 1 is essential for chloroplast iron transport and homeostasis under continuous light conditions in Arabidopsis
Summary: Chloroplasts require iron for photosynthesis; however, the transport mechanisms remain poorly understood. This study identifies Arabidopsis Fe-deficiency-induced chlorosis 1 (FIC1), localized in the chloroplast inner membrane, as essential for iron redistribution from developed to newly dev...
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| Language: | English |
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Elsevier
2025-07-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124725007132 |
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| author | Jumei Zhang Lei You Hai Liu Sheng Luo Ruiying Li Shaowu Xue Xuelei Lai Honghong Hu |
| author_facet | Jumei Zhang Lei You Hai Liu Sheng Luo Ruiying Li Shaowu Xue Xuelei Lai Honghong Hu |
| author_sort | Jumei Zhang |
| collection | DOAJ |
| description | Summary: Chloroplasts require iron for photosynthesis; however, the transport mechanisms remain poorly understood. This study identifies Arabidopsis Fe-deficiency-induced chlorosis 1 (FIC1), localized in the chloroplast inner membrane, as essential for iron redistribution from developed to newly developing leaves under continuous light (CL) conditions. CL induces FIC1 expression in newly developing leaves. fic1 displays chlorosis in newly developing leaves under iron deficiency or CL, with reduced levels of iron and photosynthetic complexes, along with an overaccumulation of reactive oxygen species (ROS). Iron supplementation alleviates these phenotypes. FIC1 functionally complements iron transport defects in fet3fet4 yeast and enhances iron uptake in Xenopus oocytes. Expressing PIC1 (PERMEASE IN CHLOROPLAST 1) under FIC1 promoter rescues both the deficits in iron/chlorophyll content and ROS accumulation in fic1. Furthermore, FIC1 modulates iron homeostasis-related gene expression under CL. These findings establish FIC1 as a chloroplast iron importer that is essential for prioritizing iron allocation to young tissues during prolonged light exposure. |
| format | Article |
| id | doaj-art-cbc34e90fc9a4084b1e01843f7495d2c |
| institution | Matheson Library |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-cbc34e90fc9a4084b1e01843f7495d2c2025-07-13T04:54:13ZengElsevierCell Reports2211-12472025-07-01447115942Fe-deficiency-induced chlorosis 1 is essential for chloroplast iron transport and homeostasis under continuous light conditions in ArabidopsisJumei Zhang0Lei You1Hai Liu2Sheng Luo3Ruiying Li4Shaowu Xue5Xuelei Lai6Honghong Hu7National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; Laboratory of Medicinal Plant, Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medicine, Hubei University of Medicine, Shiyan 442000, ChinaCollege of Life Science and Technology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Life Science and Technology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Life Science and Technology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China; Corresponding authorSummary: Chloroplasts require iron for photosynthesis; however, the transport mechanisms remain poorly understood. This study identifies Arabidopsis Fe-deficiency-induced chlorosis 1 (FIC1), localized in the chloroplast inner membrane, as essential for iron redistribution from developed to newly developing leaves under continuous light (CL) conditions. CL induces FIC1 expression in newly developing leaves. fic1 displays chlorosis in newly developing leaves under iron deficiency or CL, with reduced levels of iron and photosynthetic complexes, along with an overaccumulation of reactive oxygen species (ROS). Iron supplementation alleviates these phenotypes. FIC1 functionally complements iron transport defects in fet3fet4 yeast and enhances iron uptake in Xenopus oocytes. Expressing PIC1 (PERMEASE IN CHLOROPLAST 1) under FIC1 promoter rescues both the deficits in iron/chlorophyll content and ROS accumulation in fic1. Furthermore, FIC1 modulates iron homeostasis-related gene expression under CL. These findings establish FIC1 as a chloroplast iron importer that is essential for prioritizing iron allocation to young tissues during prolonged light exposure.http://www.sciencedirect.com/science/article/pii/S2211124725007132CP: Plants |
| spellingShingle | Jumei Zhang Lei You Hai Liu Sheng Luo Ruiying Li Shaowu Xue Xuelei Lai Honghong Hu Fe-deficiency-induced chlorosis 1 is essential for chloroplast iron transport and homeostasis under continuous light conditions in Arabidopsis Cell Reports CP: Plants |
| title | Fe-deficiency-induced chlorosis 1 is essential for chloroplast iron transport and homeostasis under continuous light conditions in Arabidopsis |
| title_full | Fe-deficiency-induced chlorosis 1 is essential for chloroplast iron transport and homeostasis under continuous light conditions in Arabidopsis |
| title_fullStr | Fe-deficiency-induced chlorosis 1 is essential for chloroplast iron transport and homeostasis under continuous light conditions in Arabidopsis |
| title_full_unstemmed | Fe-deficiency-induced chlorosis 1 is essential for chloroplast iron transport and homeostasis under continuous light conditions in Arabidopsis |
| title_short | Fe-deficiency-induced chlorosis 1 is essential for chloroplast iron transport and homeostasis under continuous light conditions in Arabidopsis |
| title_sort | fe deficiency induced chlorosis 1 is essential for chloroplast iron transport and homeostasis under continuous light conditions in arabidopsis |
| topic | CP: Plants |
| url | http://www.sciencedirect.com/science/article/pii/S2211124725007132 |
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