Enzyme Supplementation to Diets Containing Wheat Distillers’ Dried Grains with Solubles (DDGS) When Fed to Laying Hens

A study was conducted using 144 Hy-Line Brown laying hens (22 weeks old) to assess the impact of exogenous enzymes on energy utilisation and ileal nutrient digestibility in diets containing 300 g/kg wheat distillers’ dried grains with solubles (DDGS). A basal diet was prepared and divided into eight...

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Main Authors: Isobel M. Whiting, Stephen P. Rose, Ahmed M. Amerah, Vasil R. Pirgozliev
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Poultry
Subjects:
Online Access:https://www.mdpi.com/2674-1164/4/2/22
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author Isobel M. Whiting
Stephen P. Rose
Ahmed M. Amerah
Vasil R. Pirgozliev
author_facet Isobel M. Whiting
Stephen P. Rose
Ahmed M. Amerah
Vasil R. Pirgozliev
author_sort Isobel M. Whiting
collection DOAJ
description A study was conducted using 144 Hy-Line Brown laying hens (22 weeks old) to assess the impact of exogenous enzymes on energy utilisation and ileal nutrient digestibility in diets containing 300 g/kg wheat distillers’ dried grains with solubles (DDGS). A basal diet was prepared and divided into eight treatments: a control (C) and diets supplemented with 2000 units/kg xylanase (XYL), 500 units/kg phytase (PHY), and 4000 units/kg protease (PRO), individually and in combination. The diets were fed for nine days to six coops, each housing three birds. Feed intake, weight gain, and feed conversion ratio were recorded. The feed and excreta were analysed for gross energy, and the apparent metabolisable energy (AME) was calculated. On the final day, the birds were euthanised, and ileal digesta were collected, freeze-dried, and analysed for the digestibility coefficients of dry matter (DM), nitrogen, fat, and neutral detergent fibres (NDFs). XYL supplementation improved (<i>p</i> = 0.035) dietary AME but did not affect (<i>p</i> > 0.05) DM, nitrogen, fat, or NDF digestibility. No significant effects (<i>p</i> > 0.05) were observed for PHY or PRO, and no interactions (<i>p</i> > 0.05) were found between enzyme combinations. Substrates in experimental diets involving various enzyme combinations should be given careful consideration.
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spelling doaj-art-cb0448d8932c48eeadad59adbbe6503d2025-06-25T14:20:46ZengMDPI AGPoultry2674-11642025-05-01422210.3390/poultry4020022Enzyme Supplementation to Diets Containing Wheat Distillers’ Dried Grains with Solubles (DDGS) When Fed to Laying HensIsobel M. Whiting0Stephen P. Rose1Ahmed M. Amerah2Vasil R. Pirgozliev3The National Institute of Poultry Husbandry, Harper Adams University, Shropshire TF10 8NB, UKThe National Institute of Poultry Husbandry, Harper Adams University, Shropshire TF10 8NB, UKDanisco Animal Nutrition, Marlborough, Wiltshire SN8 1XN, UKThe National Institute of Poultry Husbandry, Harper Adams University, Shropshire TF10 8NB, UKA study was conducted using 144 Hy-Line Brown laying hens (22 weeks old) to assess the impact of exogenous enzymes on energy utilisation and ileal nutrient digestibility in diets containing 300 g/kg wheat distillers’ dried grains with solubles (DDGS). A basal diet was prepared and divided into eight treatments: a control (C) and diets supplemented with 2000 units/kg xylanase (XYL), 500 units/kg phytase (PHY), and 4000 units/kg protease (PRO), individually and in combination. The diets were fed for nine days to six coops, each housing three birds. Feed intake, weight gain, and feed conversion ratio were recorded. The feed and excreta were analysed for gross energy, and the apparent metabolisable energy (AME) was calculated. On the final day, the birds were euthanised, and ileal digesta were collected, freeze-dried, and analysed for the digestibility coefficients of dry matter (DM), nitrogen, fat, and neutral detergent fibres (NDFs). XYL supplementation improved (<i>p</i> = 0.035) dietary AME but did not affect (<i>p</i> > 0.05) DM, nitrogen, fat, or NDF digestibility. No significant effects (<i>p</i> > 0.05) were observed for PHY or PRO, and no interactions (<i>p</i> > 0.05) were found between enzyme combinations. Substrates in experimental diets involving various enzyme combinations should be given careful consideration.https://www.mdpi.com/2674-1164/4/2/22distillers’ dried grains with solubles (DDGS)laying hensxylanasephytaseproteaseAME
spellingShingle Isobel M. Whiting
Stephen P. Rose
Ahmed M. Amerah
Vasil R. Pirgozliev
Enzyme Supplementation to Diets Containing Wheat Distillers’ Dried Grains with Solubles (DDGS) When Fed to Laying Hens
Poultry
distillers’ dried grains with solubles (DDGS)
laying hens
xylanase
phytase
protease
AME
title Enzyme Supplementation to Diets Containing Wheat Distillers’ Dried Grains with Solubles (DDGS) When Fed to Laying Hens
title_full Enzyme Supplementation to Diets Containing Wheat Distillers’ Dried Grains with Solubles (DDGS) When Fed to Laying Hens
title_fullStr Enzyme Supplementation to Diets Containing Wheat Distillers’ Dried Grains with Solubles (DDGS) When Fed to Laying Hens
title_full_unstemmed Enzyme Supplementation to Diets Containing Wheat Distillers’ Dried Grains with Solubles (DDGS) When Fed to Laying Hens
title_short Enzyme Supplementation to Diets Containing Wheat Distillers’ Dried Grains with Solubles (DDGS) When Fed to Laying Hens
title_sort enzyme supplementation to diets containing wheat distillers dried grains with solubles ddgs when fed to laying hens
topic distillers’ dried grains with solubles (DDGS)
laying hens
xylanase
phytase
protease
AME
url https://www.mdpi.com/2674-1164/4/2/22
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AT ahmedmamerah enzymesupplementationtodietscontainingwheatdistillersdriedgrainswithsolublesddgswhenfedtolayinghens
AT vasilrpirgozliev enzymesupplementationtodietscontainingwheatdistillersdriedgrainswithsolublesddgswhenfedtolayinghens