Fraction collection of bioactive compounds from ion chromatography: No longer mission impossible

This paper demonstrates the fraction collection of the novel sugar trehalulose, using a modified ion chromatograph. The Ion Chromatography (IC) method, previously published for the analysis of trehalulose, was augmented with a suppressor and purpose-made switching valve unit. A sample of stingless b...

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Bibliographic Details
Main Authors: Hans S.A. Yates, James F. Carter, Mary T. Fletcher, Viviene S. Santiago, Ondrea Thompson, Natasha L. Hungerford
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:MethodsX
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Online Access:http://www.sciencedirect.com/science/article/pii/S2215016125000895
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Summary:This paper demonstrates the fraction collection of the novel sugar trehalulose, using a modified ion chromatograph. The Ion Chromatography (IC) method, previously published for the analysis of trehalulose, was augmented with a suppressor and purpose-made switching valve unit. A sample of stingless bee honey was then run, following the three main steps: • Separation of trehalulose fraction • Lyophilization • Confirmation of trehaluloseThe method should be applicable to not only sugar analysis but to any bioactive compound separable by IC. The authors were not able to find a similar method within currently published literature.
ISSN:2215-0161