<i>Commiphora leptophloeos</i> Bark Decoction: Phytochemical Composition, Antioxidant Capacity, and Non-Genotoxic Safety Profile

<i>Commiphora leptophloeos</i> Bark Decoction: Phytochemical Composition, Antioxidant Capacity, and Non-Genotoxic Safety Profile

<b>Background:</b> <i>Commiphora leptophloeos</i> has long been used in Latin American folk medicine for the treatment of respiratory and gastrointestinal disorders. Therefore, toxicological and phytochemical investigations are required to assess the safety and support the ev...

Full description

Saved in:
Bibliographic Details
Main Authors: José Rafael da Silva Araujo, Rafael de Felício, Camila Marinho da Silva, Palloma Lima de Oliveira, Silvany de Sousa Araújo, Laís Roberta Deroldo Sommaggio, Adriana Fabiana Corrêa da Silva, Paulo Henrique Valença Nunes, Bruno Oliveira de Veras, Erwelly Barros de Oliveira, Jaciana dos Santos Aguiar, Maria Aparecida Marin-Morales, Daniela Barretto Barbosa Trivella, Ana Maria Benko-Iseppon, Márcia Vanusa da Silva, Ana Christina Brasileiro-Vidal
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Pharmaceuticals
Subjects:
Salmonella test
imburana-de-cambão
MTT
micronuclei test
molecular network
UPLC-MS/MS phytochemical
Online Access:https://www.mdpi.com/1424-8247/18/6/863
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:<b>Background:</b> <i>Commiphora leptophloeos</i> has long been used in Latin American folk medicine for the treatment of respiratory and gastrointestinal disorders. Therefore, toxicological and phytochemical investigations are required to assess the safety and support the evidence-based use of its bark in medicinal applications. This study aimed to evaluate the aqueous bark extract of <i>C. leptophloeos</i>, focusing on its chemical composition and its antioxidant, cytotoxic, and genotoxic properties. <b>Methods</b>: The aqueous extract was obtained by decoction of dried bark samples. Phytochemical characterization was conducted using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS), and data were processed using the NP<sup>3</sup> MS Workflow 1.1.4 software, allowing for the annotation of key secondary metabolites. Antioxidant activity was assessed through multiple in vitro assays, including DPPH, ABTS, phosphomolybdenum, and reducing power tests. Cytotoxicity was evaluated using the MTT assay, while genotoxicity was investigated through the Ames test and micronucleus assay. <b>Results</b>: Phytochemical analysis revealed several flavonoids, with procyanidin B2 annotated as a major compound. The extract exhibited strong antioxidant activity, with EC<sub>50</sub> values of 5.43 μg/mL (DPPH), 12.40 μg/mL (ABTS), 35.20 μg/mL (phosphomolybdenum), and 31.27 μg/mL (reducing power). The MTT assay showed no cytotoxic effects at concentrations up to 6400 μg/mL. Furthermore, both the Ames and micronucleus assays showed the absence of genotoxic effects at concentrations up to 1600 μg/plate and 400 μg/mL, respectively. <b>Conclusions</b>: The aqueous bark extract of <i>C. leptophloeos</i> demonstrates strong antioxidant potential and a favorable safety profile, with no detectable cytotoxicity or genotoxicity at concentrations effective in antioxidant assays. Further studies are recommended to confirm and validate its traditional medicinal properties using appropriate in vivo models, followed by pre-clinical evaluations.
ISSN:1424-8247

Similar Items