Approaches to Optimization of Oligonucleotide Synthesis during Manufacturing of Preparations for Gene Diagnostics of Particularly Dangerous Infectious Diseases
Objective of the study is to investigate the impact of activator, oxidize, and unblocking solution on the quantitative yield of oligonucleotides during manufacturing of the test-system for Vibrio cholerae (ctxA+) DNA detection “GenChol”, using polymerase chain reaction.Materials and methods. The sub...
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Main Authors: | A. V. Stepanov, N. V. Mayorov, N. A. Osina, A. K. Nikiforov |
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Format: | Article |
Language: | Russian |
Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2016-09-01
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Series: | Проблемы особо опасных инфекций |
Subjects: | |
Online Access: | https://journal.microbe.ru/jour/article/view/334 |
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