Cloning, expression, and polymorphism of the ECI1 gene in various pig breeds
Abstract: The enzyme Δ3,Δ2-dienoyl-CoA isomerase (ECI1) plays a crucial role in the mitochondrial β-oxidation of fatty acids with a double-bond in odd and even positions. The ECI1 gene might be a qualified candidate for studies pertaining to lipid deposition and meat quality in swine. In the present...
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KeAi Communications Co., Ltd.
2017-08-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311916616246 |
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| author | Yun-feng LU Ji-bao CHEN Bo ZHANG Qing-gang LI Zhi-xiu WANG Hao ZHANG Ke-liang WU |
| author_facet | Yun-feng LU Ji-bao CHEN Bo ZHANG Qing-gang LI Zhi-xiu WANG Hao ZHANG Ke-liang WU |
| author_sort | Yun-feng LU |
| collection | DOAJ |
| description | Abstract: The enzyme Δ3,Δ2-dienoyl-CoA isomerase (ECI1) plays a crucial role in the mitochondrial β-oxidation of fatty acids with a double-bond in odd and even positions. The ECI1 gene might be a qualified candidate for studies pertaining to lipid deposition and meat quality in swine. In the present study, ECI1 cDNA of the Tibetan pig was obtained by in silico cloning and verified by PCR analysis. Single-nucleotide polymorphisms (SNPs) of ECI1 were screened by PCR-sequencing and genotypes of those SNPs were tested by PCR-restriction fragment length polymorphism (PCR-RFLP) in Diannan small-ear pigs (DSP, n=40), Tibetan pigs (TP, n=60) and Yorkshire pigs (YP, n=30). The expression levels of ECI1 were analyzed by real-time quantitative PCR and Western blotting in tissues of the liver, backfat, and longissimus dorsi (LD) muscle of DSP (n=8), TP (n=8) and YP (n=8). Single factor linear correlation analysis was applied separately for each breed to evaluate correlations between ECI1 gene expression in the LD muscle and intramuscular fat (IMF) content. We obtained an ECI1 gene length of 1 401 bp from the cDNA that contained a full coding region of 909 bp. Three novel SNPs (g.42425337G>A; g.42424666A>G; and g.42422755A>G) were detected, and only g.42424666A>G exhibited three genotypes among the three breeds. The ECI1 expression levels in the LD muscle of DSP and TP were significantly higher than that of YP (P<0.05). Moreover, TP had the highest ECI1 expression in backfat (P<0.01), and a positive correlation was observed between gene expression and IMF content. The results suggest that differences in ECI1 gene expression might be related to lipid deposition and meat quality in pig. |
| format | Article |
| id | doaj-art-c808c0533bf24f1389ca053d2ac1a1b5 |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2017-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-c808c0533bf24f1389ca053d2ac1a1b52025-08-02T19:11:29ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192017-08-0116817891799Cloning, expression, and polymorphism of the ECI1 gene in various pig breedsYun-feng LU0Ji-bao CHEN1Bo ZHANG2Qing-gang LI3Zhi-xiu WANG4Hao ZHANG5Ke-liang WU6College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.China; School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P.R.ChinaSchool of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P.R.ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.China; Institute of Animal Sciences and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, P.R.ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.China; Correspondence WU Ke-liang, Tel/Fax: +86-10-62734767Abstract: The enzyme Δ3,Δ2-dienoyl-CoA isomerase (ECI1) plays a crucial role in the mitochondrial β-oxidation of fatty acids with a double-bond in odd and even positions. The ECI1 gene might be a qualified candidate for studies pertaining to lipid deposition and meat quality in swine. In the present study, ECI1 cDNA of the Tibetan pig was obtained by in silico cloning and verified by PCR analysis. Single-nucleotide polymorphisms (SNPs) of ECI1 were screened by PCR-sequencing and genotypes of those SNPs were tested by PCR-restriction fragment length polymorphism (PCR-RFLP) in Diannan small-ear pigs (DSP, n=40), Tibetan pigs (TP, n=60) and Yorkshire pigs (YP, n=30). The expression levels of ECI1 were analyzed by real-time quantitative PCR and Western blotting in tissues of the liver, backfat, and longissimus dorsi (LD) muscle of DSP (n=8), TP (n=8) and YP (n=8). Single factor linear correlation analysis was applied separately for each breed to evaluate correlations between ECI1 gene expression in the LD muscle and intramuscular fat (IMF) content. We obtained an ECI1 gene length of 1 401 bp from the cDNA that contained a full coding region of 909 bp. Three novel SNPs (g.42425337G>A; g.42424666A>G; and g.42422755A>G) were detected, and only g.42424666A>G exhibited three genotypes among the three breeds. The ECI1 expression levels in the LD muscle of DSP and TP were significantly higher than that of YP (P<0.05). Moreover, TP had the highest ECI1 expression in backfat (P<0.01), and a positive correlation was observed between gene expression and IMF content. The results suggest that differences in ECI1 gene expression might be related to lipid deposition and meat quality in pig.http://www.sciencedirect.com/science/article/pii/S2095311916616246ECI1 genelipid depositionmRNA expressionpigsingle-nucleotide polymorphismWestern blotting |
| spellingShingle | Yun-feng LU Ji-bao CHEN Bo ZHANG Qing-gang LI Zhi-xiu WANG Hao ZHANG Ke-liang WU Cloning, expression, and polymorphism of the ECI1 gene in various pig breeds Journal of Integrative Agriculture ECI1 gene lipid deposition mRNA expression pig single-nucleotide polymorphism Western blotting |
| title | Cloning, expression, and polymorphism of the ECI1 gene in various pig breeds |
| title_full | Cloning, expression, and polymorphism of the ECI1 gene in various pig breeds |
| title_fullStr | Cloning, expression, and polymorphism of the ECI1 gene in various pig breeds |
| title_full_unstemmed | Cloning, expression, and polymorphism of the ECI1 gene in various pig breeds |
| title_short | Cloning, expression, and polymorphism of the ECI1 gene in various pig breeds |
| title_sort | cloning expression and polymorphism of the eci1 gene in various pig breeds |
| topic | ECI1 gene lipid deposition mRNA expression pig single-nucleotide polymorphism Western blotting |
| url | http://www.sciencedirect.com/science/article/pii/S2095311916616246 |
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