Ascite-solid model of prostate cancer and its morphological characteristics
Targeted therapy and immunotherapy are considered promising novel therapies capable of increasing the effcacy of prostate cancer (PCa) treatment. The purpose of the study was to obtain and characterize TRAMP-C2 subcutaneous and ascite-solid models of prostate cancer in C57Bl/6j mice to study specifc...
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| Format: | Article |
| Language: | Russian |
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Russian Academy of Sciences, Tomsk National Research Medical Center
2023-09-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/2680 |
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| author | E. A. Plotnikova N. B. Morozova A. V. Ryabova G. V. Trunova V. A. Khokhlova V. B. Loschenov A. A. Pankratov A. D. Kaprin |
| author_facet | E. A. Plotnikova N. B. Morozova A. V. Ryabova G. V. Trunova V. A. Khokhlova V. B. Loschenov A. A. Pankratov A. D. Kaprin |
| author_sort | E. A. Plotnikova |
| collection | DOAJ |
| description | Targeted therapy and immunotherapy are considered promising novel therapies capable of increasing the effcacy of prostate cancer (PCa) treatment. The purpose of the study was to obtain and characterize TRAMP-C2 subcutaneous and ascite-solid models of prostate cancer in C57Bl/6j mice to study specifc anti-tumor activity of the candidate molecules of targeted drugs and adjust immunotherapy strategies in an evidence-based manner. Material and Methods. We used cultured TRAMP-C2 cells in subcutaneous and ascites mouse prostate cancer models. Histological and immunohistochemical methods were used to study the tumor tissues. Results. The high in vivo growth ability of TRAMP-C2 cells was demonstrated in subcutaneous and intraperitoneal inoculation of C57Bl/6j mice. These tumors are characterized by a high reproducibility and level of PSMA expression. Histological study showed that subcutaneous and carcinomatous TRAMP-C2 tumor nodules had solid structure morphologically corresponding to low differentiated neoplasm, cytomorphological analysis of smears showed that peritoneal fuid containd a large number of rounded tumor cells, macrophages and erythrocytes. Conclusion. The obtained subcutaneous and ascite-solid models of TRAMP-C2 can be useful for the development of new ways to effectively treat cancer, including targeted and immunotherapy, as well as for the experimental study of biotherapeutic effects using PSMA as a target, and photoinduced effects. |
| format | Article |
| id | doaj-art-c7d26590b38f4eaa968b7ca623d59e40 |
| institution | Matheson Library |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2023-09-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-c7d26590b38f4eaa968b7ca623d59e402025-08-04T10:33:43ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682023-09-01224657310.21294/1814-4861-2023-22-4-65-731143Ascite-solid model of prostate cancer and its morphological characteristicsE. A. Plotnikova0N. B. Morozova1A. V. Ryabova2G. V. Trunova3V. A. Khokhlova4V. B. Loschenov5A. A. Pankratov6A. D. Kaprin7P.A. Hertsen Moscow Oncology Research Institute – branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russia; Russian Technological University – MIREA, M.V. Lomonosov Institute of Fine Chemical TechnologyP.A. Hertsen Moscow Oncology Research Institute – branch of the National Medical Research Radiological Centre of the Ministry of Health of the RussiaA.M. Prokhorov General Physics Institute of the Russian Academy of SciencesP.A. Hertsen Moscow Oncology Research Institute – branch of the National Medical Research Radiological Centre of the Ministry of Health of the RussiaP.A. Hertsen Moscow Oncology Research Institute – branch of the National Medical Research Radiological Centre of the Ministry of Health of the RussiaA.M. Prokhorov General Physics Institute of the Russian Academy of SciencesP.A. Hertsen Moscow Oncology Research Institute – branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russia; Russian Technological University – MIREA, M.V. Lomonosov Institute of Fine Chemical TechnologyP.A. Hertsen Moscow Oncology Research Institute – branch of the National Medical Research Radiological Centre of the Ministry of Health of the RussiaTargeted therapy and immunotherapy are considered promising novel therapies capable of increasing the effcacy of prostate cancer (PCa) treatment. The purpose of the study was to obtain and characterize TRAMP-C2 subcutaneous and ascite-solid models of prostate cancer in C57Bl/6j mice to study specifc anti-tumor activity of the candidate molecules of targeted drugs and adjust immunotherapy strategies in an evidence-based manner. Material and Methods. We used cultured TRAMP-C2 cells in subcutaneous and ascites mouse prostate cancer models. Histological and immunohistochemical methods were used to study the tumor tissues. Results. The high in vivo growth ability of TRAMP-C2 cells was demonstrated in subcutaneous and intraperitoneal inoculation of C57Bl/6j mice. These tumors are characterized by a high reproducibility and level of PSMA expression. Histological study showed that subcutaneous and carcinomatous TRAMP-C2 tumor nodules had solid structure morphologically corresponding to low differentiated neoplasm, cytomorphological analysis of smears showed that peritoneal fuid containd a large number of rounded tumor cells, macrophages and erythrocytes. Conclusion. The obtained subcutaneous and ascite-solid models of TRAMP-C2 can be useful for the development of new ways to effectively treat cancer, including targeted and immunotherapy, as well as for the experimental study of biotherapeutic effects using PSMA as a target, and photoinduced effects.https://www.siboncoj.ru/jour/article/view/2680prostate cancertramp-c2 cell lineprostate specific membrane antigenascite-solid prostate cancer model |
| spellingShingle | E. A. Plotnikova N. B. Morozova A. V. Ryabova G. V. Trunova V. A. Khokhlova V. B. Loschenov A. A. Pankratov A. D. Kaprin Ascite-solid model of prostate cancer and its morphological characteristics Сибирский онкологический журнал prostate cancer tramp-c2 cell line prostate specific membrane antigen ascite-solid prostate cancer model |
| title | Ascite-solid model of prostate cancer and its morphological characteristics |
| title_full | Ascite-solid model of prostate cancer and its morphological characteristics |
| title_fullStr | Ascite-solid model of prostate cancer and its morphological characteristics |
| title_full_unstemmed | Ascite-solid model of prostate cancer and its morphological characteristics |
| title_short | Ascite-solid model of prostate cancer and its morphological characteristics |
| title_sort | ascite solid model of prostate cancer and its morphological characteristics |
| topic | prostate cancer tramp-c2 cell line prostate specific membrane antigen ascite-solid prostate cancer model |
| url | https://www.siboncoj.ru/jour/article/view/2680 |
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