LC-MS Analysis of the Polyphenolic Composition and Assessment of the Antioxidant, Anti-Inflammatory and Cardioprotective Activities of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i> Extracts
This study offers a detailed assessment of the polyphenolic composition and antioxidant, anti-inflammatory, and cardioprotective properties of lyophilized extracts derived from the aerial parts of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i>. The polypheno...
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MDPI AG
2025-07-01
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| author | Mihaela-Ancuța Nechita Alina Elena Pârvu Ana Uifălean Sonia Iurian Neli-Kinga Olah Timea Henrietta Bab Rodica Vârban Vlad-Ionuț Nechita Anca Toiu Ovidiu Oniga Daniela Benedec Daniela Hanganu Ilioara Oniga |
| author_facet | Mihaela-Ancuța Nechita Alina Elena Pârvu Ana Uifălean Sonia Iurian Neli-Kinga Olah Timea Henrietta Bab Rodica Vârban Vlad-Ionuț Nechita Anca Toiu Ovidiu Oniga Daniela Benedec Daniela Hanganu Ilioara Oniga |
| author_sort | Mihaela-Ancuța Nechita |
| collection | DOAJ |
| description | This study offers a detailed assessment of the polyphenolic composition and antioxidant, anti-inflammatory, and cardioprotective properties of lyophilized extracts derived from the aerial parts of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i>. The polyphenolic content was determined through the quantification of total polyphenols, flavonoids, and caffeic acid derivatives, complemented by LC-MS profiling. The antioxidant activity was evaluated in vitro using DPPH and FRAP assays, while the in vivo antioxidant and anti-inflammatory effects were investigated in a rat model of turpentine-oil-induced acute inflammation. Cardioprotective potential was assessed in a separate rat model of isoprenaline-induced myocardial infarction. Phytochemical analysis revealed a complex polyphenolic profile for both species, with tilianin and rosmarinic acid identified as predominant compounds. In the DPPH assay, both extracts exhibited marked radical scavenging activity (IC<sub>50</sub>: 65.91 ± 1.21 μg/mL for <i>A. mexicana</i>; 68.64 ± 2.48 μg/mL for <i>A. scrophulariifolia</i>). In the in vivo assays, the administration of the extracts significantly decreased pro-oxidant biomarkers (TOS, OSI, MDA, NO) and enhanced antioxidant markers (TAC, SH groups). Furthermore, the extracts led to a significant reduction in serum levels of GOT, GPT, and CK-MB in rats subjected to myocardial injury, supporting their cardioprotective efficacy. Overall, the results suggest that <i>A. mexicana</i> and <i>A. scrophulariifolia</i> represent promising natural sources of polyphenolic compounds with potential therapeutic value in oxidative-stress-related inflammatory and cardiovascular disorders. |
| format | Article |
| id | doaj-art-c6685c25f0f64c7b973c0882f2ef1df9 |
| institution | Matheson Library |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-07-01 |
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| series | Plants |
| spelling | doaj-art-c6685c25f0f64c7b973c0882f2ef1df92025-07-25T13:34:15ZengMDPI AGPlants2223-77472025-07-011414212210.3390/plants14142122LC-MS Analysis of the Polyphenolic Composition and Assessment of the Antioxidant, Anti-Inflammatory and Cardioprotective Activities of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i> ExtractsMihaela-Ancuța Nechita0Alina Elena Pârvu1Ana Uifălean2Sonia Iurian3Neli-Kinga Olah4Timea Henrietta Bab5Rodica Vârban6Vlad-Ionuț Nechita7Anca Toiu8Ovidiu Oniga9Daniela Benedec10Daniela Hanganu11Ilioara Oniga12Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Ion Creangă Street, 12, 400010 Cluj-Napoca, RomaniaDepartment of Morpho-Functional Sciences, Discipline of Pathophysiology, “Iuliu Haţieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDepartment of Morpho-Functional Sciences, Discipline of Pathophysiology, “Iuliu Haţieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 41 V. Babes St., 400012 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, “Vasile Goldiş” Western University of Arad, 310414 Arad, RomaniaPlantExtrakt Ltd., Rădaia, 407059 Cluj-Napoca, RomaniaDepartment of Crop Science, Faculty of Agriculture, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea Mănăștur Street 3–5, 400372 Cluj-Napoca, RomaniaDepartment of Medical Informatics and Biostatistics, Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, Louis Pasteur Street 6, 400349 Cluj-Napoca, RomaniaDepartment of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Ion Creangă Street, 12, 400010 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Victor Babeș Street 41, 400010 Cluj-Napoca, RomaniaDepartment of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Ion Creangă Street, 12, 400010 Cluj-Napoca, RomaniaDepartment of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Ion Creangă Street, 12, 400010 Cluj-Napoca, RomaniaDepartment of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Ion Creangă Street, 12, 400010 Cluj-Napoca, RomaniaThis study offers a detailed assessment of the polyphenolic composition and antioxidant, anti-inflammatory, and cardioprotective properties of lyophilized extracts derived from the aerial parts of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i>. The polyphenolic content was determined through the quantification of total polyphenols, flavonoids, and caffeic acid derivatives, complemented by LC-MS profiling. The antioxidant activity was evaluated in vitro using DPPH and FRAP assays, while the in vivo antioxidant and anti-inflammatory effects were investigated in a rat model of turpentine-oil-induced acute inflammation. Cardioprotective potential was assessed in a separate rat model of isoprenaline-induced myocardial infarction. Phytochemical analysis revealed a complex polyphenolic profile for both species, with tilianin and rosmarinic acid identified as predominant compounds. In the DPPH assay, both extracts exhibited marked radical scavenging activity (IC<sub>50</sub>: 65.91 ± 1.21 μg/mL for <i>A. mexicana</i>; 68.64 ± 2.48 μg/mL for <i>A. scrophulariifolia</i>). In the in vivo assays, the administration of the extracts significantly decreased pro-oxidant biomarkers (TOS, OSI, MDA, NO) and enhanced antioxidant markers (TAC, SH groups). Furthermore, the extracts led to a significant reduction in serum levels of GOT, GPT, and CK-MB in rats subjected to myocardial injury, supporting their cardioprotective efficacy. Overall, the results suggest that <i>A. mexicana</i> and <i>A. scrophulariifolia</i> represent promising natural sources of polyphenolic compounds with potential therapeutic value in oxidative-stress-related inflammatory and cardiovascular disorders.https://www.mdpi.com/2223-7747/14/14/2122<i>Agastache mexicana</i><i>Agastache scrophulariifolia</i>polyphenolstilianinantioxidantanti-inflammatory |
| spellingShingle | Mihaela-Ancuța Nechita Alina Elena Pârvu Ana Uifălean Sonia Iurian Neli-Kinga Olah Timea Henrietta Bab Rodica Vârban Vlad-Ionuț Nechita Anca Toiu Ovidiu Oniga Daniela Benedec Daniela Hanganu Ilioara Oniga LC-MS Analysis of the Polyphenolic Composition and Assessment of the Antioxidant, Anti-Inflammatory and Cardioprotective Activities of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i> Extracts Plants <i>Agastache mexicana</i> <i>Agastache scrophulariifolia</i> polyphenols tilianin antioxidant anti-inflammatory |
| title | LC-MS Analysis of the Polyphenolic Composition and Assessment of the Antioxidant, Anti-Inflammatory and Cardioprotective Activities of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i> Extracts |
| title_full | LC-MS Analysis of the Polyphenolic Composition and Assessment of the Antioxidant, Anti-Inflammatory and Cardioprotective Activities of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i> Extracts |
| title_fullStr | LC-MS Analysis of the Polyphenolic Composition and Assessment of the Antioxidant, Anti-Inflammatory and Cardioprotective Activities of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i> Extracts |
| title_full_unstemmed | LC-MS Analysis of the Polyphenolic Composition and Assessment of the Antioxidant, Anti-Inflammatory and Cardioprotective Activities of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i> Extracts |
| title_short | LC-MS Analysis of the Polyphenolic Composition and Assessment of the Antioxidant, Anti-Inflammatory and Cardioprotective Activities of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i> Extracts |
| title_sort | lc ms analysis of the polyphenolic composition and assessment of the antioxidant anti inflammatory and cardioprotective activities of i agastache mexicana i and i agastache scrophulariifolia i extracts |
| topic | <i>Agastache mexicana</i> <i>Agastache scrophulariifolia</i> polyphenols tilianin antioxidant anti-inflammatory |
| url | https://www.mdpi.com/2223-7747/14/14/2122 |
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