LC-MS Analysis of the Polyphenolic Composition and Assessment of the Antioxidant, Anti-Inflammatory and Cardioprotective Activities of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i> Extracts
This study offers a detailed assessment of the polyphenolic composition and antioxidant, anti-inflammatory, and cardioprotective properties of lyophilized extracts derived from the aerial parts of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i>. The polypheno...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/14/2122 |
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| Summary: | This study offers a detailed assessment of the polyphenolic composition and antioxidant, anti-inflammatory, and cardioprotective properties of lyophilized extracts derived from the aerial parts of <i>Agastache mexicana</i> and <i>Agastache scrophulariifolia</i>. The polyphenolic content was determined through the quantification of total polyphenols, flavonoids, and caffeic acid derivatives, complemented by LC-MS profiling. The antioxidant activity was evaluated in vitro using DPPH and FRAP assays, while the in vivo antioxidant and anti-inflammatory effects were investigated in a rat model of turpentine-oil-induced acute inflammation. Cardioprotective potential was assessed in a separate rat model of isoprenaline-induced myocardial infarction. Phytochemical analysis revealed a complex polyphenolic profile for both species, with tilianin and rosmarinic acid identified as predominant compounds. In the DPPH assay, both extracts exhibited marked radical scavenging activity (IC<sub>50</sub>: 65.91 ± 1.21 μg/mL for <i>A. mexicana</i>; 68.64 ± 2.48 μg/mL for <i>A. scrophulariifolia</i>). In the in vivo assays, the administration of the extracts significantly decreased pro-oxidant biomarkers (TOS, OSI, MDA, NO) and enhanced antioxidant markers (TAC, SH groups). Furthermore, the extracts led to a significant reduction in serum levels of GOT, GPT, and CK-MB in rats subjected to myocardial injury, supporting their cardioprotective efficacy. Overall, the results suggest that <i>A. mexicana</i> and <i>A. scrophulariifolia</i> represent promising natural sources of polyphenolic compounds with potential therapeutic value in oxidative-stress-related inflammatory and cardiovascular disorders. |
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| ISSN: | 2223-7747 |