Preparation, Physicochemical Properties and Intestinal Flora Regulatory Activity of Aqueous Extracts from Differently Pretreated Naematelia aurantialba
Aqueous extracts (named NAP, NAPS and NAPH, respectively) from Naematelia aurantialba fruiting bodies subjected to three different pretreatments, namely, conventional grinding, steam explosion (SE) and high-pressure homogenization (HPH) were obtained by hot water extraction, and their yields, polysa...
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China Food Publishing Company
2025-06-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-11-019.pdf |
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| author | LIU Xiaoyu, LIU Liping, LIU Yanfang, FENG Jie, YANG Linlei, CAO Yao, ZHANG Jingsong, KANG Ji |
| author_facet | LIU Xiaoyu, LIU Liping, LIU Yanfang, FENG Jie, YANG Linlei, CAO Yao, ZHANG Jingsong, KANG Ji |
| author_sort | LIU Xiaoyu, LIU Liping, LIU Yanfang, FENG Jie, YANG Linlei, CAO Yao, ZHANG Jingsong, KANG Ji |
| collection | DOAJ |
| description | Aqueous extracts (named NAP, NAPS and NAPH, respectively) from Naematelia aurantialba fruiting bodies subjected to three different pretreatments, namely, conventional grinding, steam explosion (SE) and high-pressure homogenization (HPH) were obtained by hot water extraction, and their yields, polysaccharide, uronic acid and protein contents, molecular mass distributions and monosaccharide compositions were compared. A simulated in vitro fermentation model was employed to investigate the effects of these extracts on the intestinal flora using high-throughput 16S rRNA gene sequencing. The results showed that the yields and polysaccharide contents of NAPS (64.15% and 75.87%, respectively) and NAPH (61.64% and 72.56%, respectively) were evidently higher than those of NAP. The molecular mass (mw) of NAP was 1.731 × 106 g/mol, which was higher than that of NAPH, and NAPS exhibited new characteristic peaks. NAP, NAPS and NAPH were all composed of mannose, xylose, glucose and glucuronic acid, and the monosaccharide composition changed slightly after SE and HPH. All extracts changed the composition of the intestinal flora. Both NAPS and NAP had an advantage in increasing the abundance of Macroomonas, NAPH increased the abundance of Bifidobacterium and Lactobacillus (P < 0.05), and NAP increased the abundance of Butyricicoccus (P < 0.05). The yields of short-chain fatty acids (SCFAs) significantly increased with the addition of each extract. Notably, butyric acid content was higher in the F-NAP group, while acetic acid and propionic acid contents were higher in the F-NAPH and F-NAPS groups. This study provides a reference for the efficient preparation of aqueous extracts from N. aurantialba and its application for regulating the intestinal flora. |
| format | Article |
| id | doaj-art-c4c734f2ef5d44019eb74b841d28dbee |
| institution | Matheson Library |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-c4c734f2ef5d44019eb74b841d28dbee2025-07-21T02:25:05ZengChina Food Publishing CompanyShipin Kexue1002-66302025-06-01461118018910.7506/spkx1002-6630-20241118-134Preparation, Physicochemical Properties and Intestinal Flora Regulatory Activity of Aqueous Extracts from Differently Pretreated Naematelia aurantialbaLIU Xiaoyu, LIU Liping, LIU Yanfang, FENG Jie, YANG Linlei, CAO Yao, ZHANG Jingsong, KANG Ji0(1. College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China;2. Key Laboratory of Edible Fungal Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, National Engineering Research Center of Edible Fungi, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;3. Yunnan Junshijie Biotechnology Co. Ltd., Kunming 651708, China)Aqueous extracts (named NAP, NAPS and NAPH, respectively) from Naematelia aurantialba fruiting bodies subjected to three different pretreatments, namely, conventional grinding, steam explosion (SE) and high-pressure homogenization (HPH) were obtained by hot water extraction, and their yields, polysaccharide, uronic acid and protein contents, molecular mass distributions and monosaccharide compositions were compared. A simulated in vitro fermentation model was employed to investigate the effects of these extracts on the intestinal flora using high-throughput 16S rRNA gene sequencing. The results showed that the yields and polysaccharide contents of NAPS (64.15% and 75.87%, respectively) and NAPH (61.64% and 72.56%, respectively) were evidently higher than those of NAP. The molecular mass (mw) of NAP was 1.731 × 106 g/mol, which was higher than that of NAPH, and NAPS exhibited new characteristic peaks. NAP, NAPS and NAPH were all composed of mannose, xylose, glucose and glucuronic acid, and the monosaccharide composition changed slightly after SE and HPH. All extracts changed the composition of the intestinal flora. Both NAPS and NAP had an advantage in increasing the abundance of Macroomonas, NAPH increased the abundance of Bifidobacterium and Lactobacillus (P < 0.05), and NAP increased the abundance of Butyricicoccus (P < 0.05). The yields of short-chain fatty acids (SCFAs) significantly increased with the addition of each extract. Notably, butyric acid content was higher in the F-NAP group, while acetic acid and propionic acid contents were higher in the F-NAPH and F-NAPS groups. This study provides a reference for the efficient preparation of aqueous extracts from N. aurantialba and its application for regulating the intestinal flora.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-11-019.pdfaqueous extract; polysaccharides; molecular mass distribution; steam explosion; high pressure homogenization; intestinal flora |
| spellingShingle | LIU Xiaoyu, LIU Liping, LIU Yanfang, FENG Jie, YANG Linlei, CAO Yao, ZHANG Jingsong, KANG Ji Preparation, Physicochemical Properties and Intestinal Flora Regulatory Activity of Aqueous Extracts from Differently Pretreated Naematelia aurantialba Shipin Kexue aqueous extract; polysaccharides; molecular mass distribution; steam explosion; high pressure homogenization; intestinal flora |
| title | Preparation, Physicochemical Properties and Intestinal Flora Regulatory Activity of Aqueous Extracts from Differently Pretreated Naematelia aurantialba |
| title_full | Preparation, Physicochemical Properties and Intestinal Flora Regulatory Activity of Aqueous Extracts from Differently Pretreated Naematelia aurantialba |
| title_fullStr | Preparation, Physicochemical Properties and Intestinal Flora Regulatory Activity of Aqueous Extracts from Differently Pretreated Naematelia aurantialba |
| title_full_unstemmed | Preparation, Physicochemical Properties and Intestinal Flora Regulatory Activity of Aqueous Extracts from Differently Pretreated Naematelia aurantialba |
| title_short | Preparation, Physicochemical Properties and Intestinal Flora Regulatory Activity of Aqueous Extracts from Differently Pretreated Naematelia aurantialba |
| title_sort | preparation physicochemical properties and intestinal flora regulatory activity of aqueous extracts from differently pretreated naematelia aurantialba |
| topic | aqueous extract; polysaccharides; molecular mass distribution; steam explosion; high pressure homogenization; intestinal flora |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-11-019.pdf |
| work_keys_str_mv | AT liuxiaoyuliulipingliuyanfangfengjieyanglinleicaoyaozhangjingsongkangji preparationphysicochemicalpropertiesandintestinalfloraregulatoryactivityofaqueousextractsfromdifferentlypretreatednaemateliaaurantialba |