MDA-MB-231 cell apoptosis in vitro induced by dihydromyricetin
To investigate the effect of dihydromyricetin (DMY) induction on MDA-MB-231 cell apoptosis and its mechanism, the changes of cell morphological characteristics were observed under inverted microscope. Apoptosis, intracellular Ca<sup>2+</sup> level and the mitochondrial innermembrane pote...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2012-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.03.010 |
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| Summary: | To investigate the effect of dihydromyricetin (DMY) induction on MDA-MB-231 cell apoptosis and its mechanism, the changes of cell morphological characteristics were observed under inverted microscope. Apoptosis, intracellular Ca<sup>2+</sup> level and the mitochondrial innermembrane potential (ΔΨm) were analyzed by flow cytometry (FCM). Western blotting was employed to detect the expression of caspase-3 and caspase-9. The results showed that the cell size and volume became smaller with increasing concentration of DMY. Increased Sub-G1 peak and intracellular Ca<sup>2+</sup> level, as well as the decreased mitochondrial ΔΨmin MDA-MB-231 cells were obviously observed in a dose-dependent manner. Western blotting analysis showed that DMY dose-dependently increased the expression of caspase-3 and caspase-9 in MDA-MB-231 cells. These results indicate that DMY can induce MDA-MB-231 cell apoptosis by the mitochondrial pathway. |
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| ISSN: | 1008-9209 2097-5155 |