Evaluation of diagnostic potential of immunoreactive epitopes of the Omptin protease family by using a peptide library
The construction of highly sensitive new-generation diagnostic test systems requires the use of modern innovative technologies that are capable of detecting infectious agents at the molecular level by identifying specific antibodies to individual immunoreactive epitopes. The development of such immu...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Ministry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products»
2018-02-01
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| Series: | Биопрепараты: Профилактика, диагностика, лечение |
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| Online Access: | https://www.biopreparations.ru/jour/article/view/101 |
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| _version_ | 1839581841031430144 |
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| author | V. A. Feodorova M. A. Khizhnyakova S. S. Zaitsev A. M. Lyapina L. V. Sayapina E. P. Lyapina O. V. Ulyanova V. L. Motin |
| author_facet | V. A. Feodorova M. A. Khizhnyakova S. S. Zaitsev A. M. Lyapina L. V. Sayapina E. P. Lyapina O. V. Ulyanova V. L. Motin |
| author_sort | V. A. Feodorova |
| collection | DOAJ |
| description | The construction of highly sensitive new-generation diagnostic test systems requires the use of modern innovative technologies that are capable of detecting infectious agents at the molecular level by identifying specific antibodies to individual immunoreactive epitopes. The development of such immunoassays is based on molecular mapping of a target antigen using a library of short overlapping peptides aimed at identification of both epitopes specific for a particular pathogen, and those common to the group of infectious agents. The article describes new approaches that are based on the results of mapping of the Omptin group model protein that could be critical for improving retrospective laboratory diagnosis of infectious diseases caused by bacteria of the Enterobacteriaceae family. The article discusses the prospects for assessing the applicability of the peptide ELISA experimental version, designated as p6/24-Omptin-TIFA, for the detection of antibodies to diagnostically significant Omptin epitopes in the serum of people who have had enterobacterial infections, in order to introduce it into healthcare practices. |
| format | Article |
| id | doaj-art-c3446f853d4a4a57b9a07fa2e9c32406 |
| institution | Matheson Library |
| issn | 2221-996X 2619-1156 |
| language | Russian |
| publishDate | 2018-02-01 |
| publisher | Ministry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» |
| record_format | Article |
| series | Биопрепараты: Профилактика, диагностика, лечение |
| spelling | doaj-art-c3446f853d4a4a57b9a07fa2e9c324062025-08-04T10:16:01ZrusMinistry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products»Биопрепараты: Профилактика, диагностика, лечение2221-996X2619-11562018-02-01173180186101Evaluation of diagnostic potential of immunoreactive epitopes of the Omptin protease family by using a peptide libraryV. A. Feodorova0M. A. Khizhnyakova1S. S. Zaitsev2A. M. Lyapina3L. V. Sayapina4E. P. Lyapina5O. V. Ulyanova6V. L. Motin7Federal Research Center for Virology and Microbiology (FRCVM) - Branch in Saratov; N. I. Vavilov Saratov State Agrarian UniversityFederal Research Center for Virology and Microbiology (FRCVM) - Branch in Saratov; N. I. Vavilov Saratov State Agrarian UniversityFederal Research Center for Virology and Microbiology (FRCVM) - Branch in SaratovFederal Research Center for Virology and Microbiology (FRCVM) - Branch in SaratovScientific Centre for Expert Evaluation of Medicinal ProductsSaratov State Medical University named after N. I. RazumovskyFederal Research Center for Virology and Microbiology (FRCVM) - Branch in SaratovUniversity of Texas Medical BranchThe construction of highly sensitive new-generation diagnostic test systems requires the use of modern innovative technologies that are capable of detecting infectious agents at the molecular level by identifying specific antibodies to individual immunoreactive epitopes. The development of such immunoassays is based on molecular mapping of a target antigen using a library of short overlapping peptides aimed at identification of both epitopes specific for a particular pathogen, and those common to the group of infectious agents. The article describes new approaches that are based on the results of mapping of the Omptin group model protein that could be critical for improving retrospective laboratory diagnosis of infectious diseases caused by bacteria of the Enterobacteriaceae family. The article discusses the prospects for assessing the applicability of the peptide ELISA experimental version, designated as p6/24-Omptin-TIFA, for the detection of antibodies to diagnostically significant Omptin epitopes in the serum of people who have had enterobacterial infections, in order to introduce it into healthcare practices.https://www.biopreparations.ru/jour/article/view/101лабораторная диагностикапептидный эррейтвердофазный иммуноферментный анализomptinenterobacteriaceaeретроспективная диагностикаиммунореактивный эпитопlaboratory diagnosispeptide arrayelisaomptinenterobacteriaceaeretrospective diagnosisimmunoreactive epitope |
| spellingShingle | V. A. Feodorova M. A. Khizhnyakova S. S. Zaitsev A. M. Lyapina L. V. Sayapina E. P. Lyapina O. V. Ulyanova V. L. Motin Evaluation of diagnostic potential of immunoreactive epitopes of the Omptin protease family by using a peptide library Биопрепараты: Профилактика, диагностика, лечение лабораторная диагностика пептидный эррей твердофазный иммуноферментный анализ omptin enterobacteriaceae ретроспективная диагностика иммунореактивный эпитоп laboratory diagnosis peptide array elisa omptin enterobacteriaceae retrospective diagnosis immunoreactive epitope |
| title | Evaluation of diagnostic potential of immunoreactive epitopes of the Omptin protease family by using a peptide library |
| title_full | Evaluation of diagnostic potential of immunoreactive epitopes of the Omptin protease family by using a peptide library |
| title_fullStr | Evaluation of diagnostic potential of immunoreactive epitopes of the Omptin protease family by using a peptide library |
| title_full_unstemmed | Evaluation of diagnostic potential of immunoreactive epitopes of the Omptin protease family by using a peptide library |
| title_short | Evaluation of diagnostic potential of immunoreactive epitopes of the Omptin protease family by using a peptide library |
| title_sort | evaluation of diagnostic potential of immunoreactive epitopes of the omptin protease family by using a peptide library |
| topic | лабораторная диагностика пептидный эррей твердофазный иммуноферментный анализ omptin enterobacteriaceae ретроспективная диагностика иммунореактивный эпитоп laboratory diagnosis peptide array elisa omptin enterobacteriaceae retrospective diagnosis immunoreactive epitope |
| url | https://www.biopreparations.ru/jour/article/view/101 |
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