Comparative analysis and application of novel spectrophotometric approaches and bioassay for fast macrolide quantification in table
Novel spectrophotometric methods and bioassays have been developed and validated for clarithromycin quantitation in tablets. Spectrophotometric techniques were based on charge transfer complexation through naphthoquinone derivatives. Reactions were carried out in alkaline medium using 1,2-naphthoqui...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Academy of Sciences of Moldova, Institute of Chemistry
2025-07-01
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| Series: | Chemistry Journal of Moldova: General, Industrial and Ecological Chemistry |
| Subjects: | |
| Online Access: |
http://cjm.ichem.md/comparative-analysis-and-application-of-novel-spectrophotometric-approaches
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| Summary: | Novel spectrophotometric methods and bioassays have been developed and validated for clarithromycin quantitation in tablets. Spectrophotometric techniques were based on charge transfer complexation through naphthoquinone derivatives. Reactions were carried out in alkaline medium using 1,2-naphthoquinone-4-sulphonate and phylloquinone, which showed an absorption maximum at 452 and 455 nm, respectively. While bioassay was conducted dependent on the inhibitory effect upon the strain of Bacillus subtilis ATCC 9372, by applying cylinder-plate. Linear calibration curves with correlation coefficients of 0.9980-0.9998 were obtained. Molar absorptivity and Sandell's sensitivity were less than 10.73 L/mole/cm and 0.0099 μg/cm, respectively, with a detection limit down to 0.27 μg/mL and quantification limits of 0.68-0.78 μg/mL. The validation of the developed methods was performed for selectivity, precision, accuracy and robustness. Recoveries were found between 97.5-101.9% with % RSD being bellow to 3.5%. A comparative analysis was established and the methods were successfully applied for clarithromycin quantification in dosage forms. |
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| ISSN: | 1857-1727 2345-1688 |